Thymosin Beta-4
Taken to heal injuries faster
TB4; often sold as “TB-500”
Match every finding to its exact substance, formulation, route and study population. A related molecule or approved medicine does not validate a catalog vial.
Simplified drawing of a chain of 43 amino acids
At a glance
Why people take it. And what backs it up.
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Heal injuries faster
Animal studies only
Often sold as ‘TB-500’ and injected, usually with BPC-157, to speed healing of muscle, tendon and ligament injuries.
Sources (4)
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ForumReddit r/Peptides: “Day 2 - BPC 157 & TB4 injury recovery” (April 2024) (opens in a new tab)
“Day 2 - BPC 157 & TB4 injury recovery”
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ClinicBoost Healing (clinic): Thymosin Beta 4 patient sheet (opens in a new tab)
“Thymosin Beta 4 is most often prescribed for acute injury, surgical repair and for senior athletes.”
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StudyXu et al., Regulatory Peptides, 2013, rat knee-ligament study (PubMed abstract) (opens in a new tab)
“Local administration of Tβ4 promotes the healing process of MCL, both histologically and mechanically, in a rat model.”
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“Despite promising findings in animal studies, the claimed benefits of emerging peptide supplements for musculoskeletal recovery and performance remain unsubstantiated by current human trials.”
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Recover from hard training
People report it, not studied
Athletes and lifters use it hoping for less soreness and stiffness between hard sessions, though it is banned in tested sport.
Sources (3)
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ForumReddit r/Peptides: comment in “tb500/tb4 dosing help plse” (May 2024) (opens in a new tab)
“I’ve just started taking a very, very small amount of TB500 daily. Like 100 micrograms. I’ve noticed immediate improvements to muscular recovery.”
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ClinicPRAMAH / The Haven (Tampa clinic): TB-500 (thymosin beta-4) page (opens in a new tab)
“Since then, an increasing number of athletes and biohackers have been using thymosin all for the very same reasons of repair, reduction of inflammation and to speed up recovery.”
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RegulatorWorld Anti-Doping Agency, Prohibited List 2026 (section S2) (opens in a new tab)
“Thymosin-ß4 and its derivatives e.g. TB-500”
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Recover after surgery
Animal studies only
People also take it around operations, often with BPC-157, hoping to heal faster.
Sources (4)
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“30 days post op distal bicep tear- BPC/ tb-4 results”
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“Bro I took TB-500 (could have been TB4) after my surgery and it was awesome.”
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ClinicBoost Healing (clinic): Thymosin Beta 4 patient sheet (opens in a new tab)
“Thymosin Beta 4 is most often prescribed for acute injury, surgical repair and for senior athletes.”
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StudyXu et al., Regulatory Peptides, 2013, rat knee-ligament study (PubMed abstract) (opens in a new tab)
“Local administration of Tβ4 promotes the healing process of MCL, both histologically and mechanically, in a rat model.”
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Heal skin wounds
Small trials, mixed results
Clinics also offer it for slow-healing wounds; trials tested it only as a skin treatment, on leg and pressure ulcers.
Sources (4)
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ClinicPRAMAH / The Haven (Tampa clinic): TB-500 (thymosin beta-4) page (opens in a new tab)
“For a stressed and injured person, TB 500 can offer an extremely desirable wound healing effects.”
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ClinicFormBlends (telehealth peptide provider): Thymosin Beta-4 for wound healing (opens in a new tab)
“At FormBlends, our physicians design wound healing protocols using evidence-based peptide therapy.”
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“Efficacy findings from this Phase 2 study suggest that a Tbeta4 dose of 0.03% may have the potential to accelerate wound healing”
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“in two phase 2 clinical trials of stasis and pressure ulcers, it was found to accelerate healing by almost a month in those patients that did heal.”
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Evidence briefing
Sources checkedWhat the sources support.
Thymosin beta-4 is the full 43-amino-acid protein. Human evidence concerns investigational RGN-259 eye drops; it does not establish benefit or safety for injectable TB4 vials, and no FDA-approved TB4 product was identified.
Full-length TB4 differs from the seven-amino-acid TB-500 fragment; eye-drop results do not transfer to injection.
The research. What’s been studied.
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Animal & lab studies
Wound-healing research
Animal studies report faster skin healing and changes in cell migration; clinical benefit for injected TB4 has not been established.
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Early human studies
Eye-drop research
Investigational thymosin beta-4 eye drops were tested for dry eye and corneal disease, with mixed trial results.
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Unverified
Full-length protein
Thymosin beta-4 is distinct from the seven-amino-acid TB-500 fragment sometimes sold under a similar name.
What do these labels mean?
- Human evidence: approved-drug data or larger human studies, within the populations and uses studied.
- Early human studies: small, preliminary or limited human studies.
- Animal & lab studies: animal or laboratory findings that do not establish effects in people.
- Unverified: a proposed use or claim without verified supporting clinical evidence.
How each claim was reviewed (5)
Reported effects and evidence.
A citation may support, qualify or correct a claim. Read the study scope; a laboratory result is not an established benefit in people. Each review covers a claim as it was first collected; the tiles above show the collection’s current wording.
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Preclinical wound experiments
Review of “Faster wound healing”
Full-length thymosin beta-4 accelerated repair measures in a rat skin-wound experiment.
Limitations and applicability. Verified original supports rat dermal repair; source eye-injury claim separately.
Original collected claim and review history
Original title: Faster wound healing
Sped healing of skin and eye injuries in animals.
Original label: animal. Preserved for traceability; the reviewed wording above qualifies this record.
Record tb4:benefit:1 · Annotated 2026-10-01 · Review method
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Cell/animal mechanism
Review of “Moves repair cells into injuries”
Full-length thymosin beta-4 interacts with actin and affected cell migration in preclinical experiments.
Limitations and applicability. Cell migration and actin binding are mechanistic findings, not independently established human tissue-repair benefits.
Original collected claim and review history
Original title: Moves repair cells into injuries
Helps cells migrate into damaged tissue by controlling actin, part of the cell skeleton.
Original label: animal. Preserved for traceability; the reviewed wording above qualifies this record.
Record tb4:benefit:2 · Annotated 2026-10-01 · Review method
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Preclinical mouse corneal-injury experiment
Review of “Less inflammation and scarring”
Full-length thymosin beta-4 reduced inflammatory measures in a mouse corneal-injury model; source the scarring claim separately.
Limitations and applicability. Verified source supports decreased inflammation after alkali injury; scar reduction needs a separate endpoint/source.
Original collected claim and review history
Original title: Less inflammation and scarring
Reduced inflammation and scar formation in animal studies.
Original label: animal. Preserved for traceability; the reviewed wording above qualifies this record.
Record tb4:benefit:3 · Annotated 2026-10-01 · Review method
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Preclinical mouse cardiac injury
Review of “Heart repair”
In mice after coronary ligation, full-length thymosin beta-4 improved cardiac survival/function measures.
Limitations and applicability. Mouse coronary-ligation findings do not establish human post-infarction repair.
Original collected claim and review history
Original title: Heart repair
Protected heart tissue after heart attacks in animal studies.
Original label: animal. Preserved for traceability; the reviewed wording above qualifies this record.
Record tb4:benefit:4 · Annotated 2026-10-01 · Review method
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Human topical RCT; mixed endpoints
Review of “Eye surface repair”
Full-length topical thymosin beta-4 has mixed human eye-study findings, including a trial with no significant primary-endpoint benefit.
Limitations and applicability. 2015 trial missed both primary endpoints despite favorable secondary endpoints; do not imply systemic or fragment efficacy.
Original collected claim and review history
Original title: Eye surface repair
Tested as eye drops for dry eye and corneal damage, with mixed results.
Original label: early. Preserved for traceability; the reviewed wording above qualifies this record.
Record tb4:benefit:5 · Annotated 2026-10-01 · Review method
The research in detail
Evidence category
Human trials, not approved
Animal studies and human trials of RGN-259 eye drops provide different levels of evidence for different routes. Eye-drop trials had mixed results; no injectable TB4 product has been approved.
Evidence is assessed claim by claim above. Regulatory approval, study design, findings and relevance are separate questions; there is no single evidence score.
Risks and unknowns. What to know first.
Collected cautions follow. These are not an exhaustive or independently validated safety assessment; claim references do not automatically support every caution.
- Injected safety in people is unstudied.
- Theoretical tumor-growth concern because it promotes blood vessels and cell migration.
What people report
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Regulator
Thymosin beta-4 and its derivatives, including TB-500, are banned in sport at all times (World Anti-Doping Agency category S2).
Source: World Anti-Doping Agency Prohibited List (opens in a new tab)
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Regulator
FDA’s compounding safety list names only the 7-amino-acid fragment (‘Thymosin beta-4, fragment (LKKTETQ), also known as TB-500’), not full-length thymosin beta-4, so FDA has published no compounding review of the full protein.
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Regulator
In July 2026 an FDA advisory committee voted 8–6 to recommend allowing ‘TB-500’ for compounding (wound healing). FDA’s briefing defined TB-500 as the 17–23 fragment, so the vote does not cover full-length thymosin beta-4; FDA has not decided.
Source: AJMC, FDA Panel Backs 6 Peptides for Compounding (July 2026) (opens in a new tab)
Show all 8 cautionsShow fewer cautions
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User reports
One user’s diastolic blood pressure dropped enough to cause symptoms after raising full-length TB4 to 2 mg a day.
Source: Reddit r/Peptides thread: blood pressure drop on full-length TB4 (opens in a new tab)
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User reports
Users report heavy tiredness or lethargy on TB4, sometimes together with BPC-157.
Source: Reddit r/Peptides thread ‘Extreme lethargy in BPC157 and TB4’ (opens in a new tab)
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User reports
Palpitations and an irregular-feeling heartbeat after raising TB4 to 750 mcg a day alongside more BPC-157.
Source: Reddit r/Peptides thread on palpitations with BPC-157 and TB4 (opens in a new tab)
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Case report
One FDA adverse-event report describes widespread skin and gum darkening after a week of a ‘research only’ BPC-157 + TB-500 blend taken twice a day, which returned when the product was restarted.
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Study
Lab and tissue studies link high thymosin beta-4 activity to tumour growth and spread. Whether injected thymosin beta-4 affects cancer risk in people is unknown.
Published dosing records. What the trials used.
Historical label and trial records for the named product, route, population and indication. These records are not a personal schedule or a validated regimen for a catalog vial. Source attribution does not establish applicability.
Phase 3 eye drops
Phase 3 trial (SEER-1) of 0.1% eye drops in people with neurotrophic keratopathy, a nerve-related disease of the eye surface (International Journal of Molecular Sciences, 2022)
Source: Sosne et al., International Journal of Molecular Sciences, 2022 (SEER-1) (opens in a new tab)
- Weeks 1–4
- 0.1%
- How
- Eye drops
- How often
- Five times a day
- How long
- 4 weeks in the trial
- These were eye drops, not injections. No injected dose has been established.
- The drops went into the affected eye or eyes.
- The trial missed its main goal: 6 of 10 treated people healed fully by 4 weeks, versus 1 of 8 on placebo, which was not statistically significant.
- In a safety study, healthy volunteers got 42, 140, 420 or 1260 mg into a vein, once and then daily for 14 days, with no dose-limiting side effects (Ruff et al., 2010).
Phase 2 dry eye
Phase 2 trial of 0.1% eye drops in people with moderate to severe dry eye (Clinical Ophthalmology, 2015)
Source: Sosne and Ousler, Clinical Ophthalmology, 2015 (opens in a new tab)
- Days 1–28
- 0.1%
- How
- Eye drops
- How often
- Twice a day, 1–2 drops in each eye
- How long
- 28 days in the trial
- These were eye drops, not injections. No injected dose has been established.
- The drops did not beat placebo on the two main measures, though some other measures improved.
- A smaller trial in severe dry eye used the same 0.1% drops six times a day for 28 days (Sosne et al., Cornea, 2015).
These doses were tested in clinical trials under medical supervision. They aren’t approved for use, and a research vial is not the product that was tested.
Community protocols. Reported online. Not clinically validated.
Community reports Collection date: 30 Sep 2026Forum users and vendor guides mostly inject about 2–2.5 mg twice a week (about 5 mg a week) for 4–6 weeks and then about half that, while clinics and compounding pharmacies give 450–750 mcg a day for 20–30 days.
More about this
Forum testers say most ‘TB-500’ sold is actually full-length thymosin beta-4, so many reported ‘TB-500’ doses really apply to this peptide.
Twice-weekly loading, then maintenance
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
- Forum: Reddit r/Peptides, thread ‘Difference between TB4 and TB500 & Dosing Recommendation’ (2023)
- Clinic: PRAMAH / The Haven (Tampa clinic), TB-500 page
- Forum: Reddit r/Peptides, thread ‘BPC-157?oral vs injectable. Which is better?’ (2025)
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- About 2–2.5 mg per shot (roughly 5 mg a week); some go to 5 mg per shot or up to 20 mg a week
- How often
- Twice a week (some three times) during loading; then once a week or every other week
- Route
- Injection under the skin (some inject into a muscle)
- How long
- Loading for 1–6 weeks (4–6 weeks is most often quoted), then maintenance
- Cycle
- Maintenance at about half the weekly loading dose
- Ramp
- Loading phase first, then taper to maintenance
- Mixing
- Research vials of 5 or 10 mg, mixed with 1–3 mL bacteriostatic water
- Many users following this schedule do not know whether their ‘TB-500’ is the full protein or the fragment.
- Some users say it is a mistake to dose full-length thymosin beta-4 only twice a week, because they believe its half-life is only a few hours.
Sources (3)
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“The most common one I’ve come across is 2.5mg twice per week for a total of 5mg per week”
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ClinicPRAMAH / The Haven (Tampa clinic), TB-500 page (opens in a new tab)
“Doses can range from 5 mg to 20 mg per week by SQ or IM injections divided into 2-3 injections per week.”
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ForumReddit r/Peptides, thread ‘BPC-157?oral vs injectable. Which is better?’ (2025) (opens in a new tab)
“Monday: TB-500 2.5mg / BPC-157 250mcg”
Daily compounded course (clinic)
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
- Vendor: Tailor Made Compounding peptide catalog (compounding pharmacy, 2019)
- Clinic: Boost Healing (clinic), Thymosin Beta 4 patient sheet
- Practitioner: American Academy of Anti-Aging Medicine (A4M), Thymosin beta 4 professional monograph
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- 450–750 mcg a day (range quoted by one professional society: 300 mcg upward)
- How often
- Once a day
- Route
- Injection under the skin, usually in the belly
- How long
- 20–30 days (for example 750 mcg for 20 days or 450 mcg for 30 days)
- Cycle
- One monograph: no more than 3 months without a break (3 months on and 6 weeks off, or 6 weeks on and 6 weeks off)
- Mixing
- Compounded as a ready-mixed 3 mg per mL solution in a 5 mL vial
- The clinic sheet quoted here copies the 2019 Tailor Made Compounding catalog almost word for word, showing how one pharmacy’s protocol spread.
- The American Academy of Anti-Aging Medicine (A4M) monograph’s range runs from ‘300 mcg – 1 gram daily’. The ‘gram’ is probably a typing error for milligram, but Reddit users repeat it.
Sources (3)
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VendorTailor Made Compounding peptide catalog (compounding pharmacy, 2019) (opens in a new tab)
“Content & Potency: 3000mcg/ml subcutaneous injection provided in a 5ml vial. Suggested dosage: Inject 0.25ml subcutaneously daily for 20 days.”
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ClinicBoost Healing (clinic), Thymosin Beta 4 patient sheet (opens in a new tab)
“Inject 0.15 ml (0.45 mg) subcutaneously daily for 30 days or 0.25 ml (0.75mg) subcutaneously daily for 20 days.”
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PractitionerAmerican Academy of Anti-Aging Medicine (A4M), Thymosin beta 4 professional monograph (opens in a new tab)
“300 mcg – 1 gram daily, SubQ”
Show all 5 protocolsShow fewer protocols
Daily or twice-daily small doses (forums)
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
- Forum: Reddit r/Peptides, thread ‘Difference between TB4 and TB500 & Dosing Recommendation’ (2023)
- Forum: Reddit r/Peptides, thread ‘BPC-157 and TB4 dosage for female.’ (2024)
- Forum: MESO-Rx forum, ‘A comprehensive tb500 frag thread’ (2025)
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- 250–750 mcg per shot; 300 mcg–1.5 mg a day
- How often
- Once or twice a day
- Route
- Injection under the skin
- Ramp
- One forum user started at 125 mcg twice a day and worked up to 750 mcg twice a day
- Users who believe full-length thymosin beta-4 is short-lived dose it like BPC-157, often in the same syringe.
Sources (3)
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“I would inject your TB4 at the same dose (250mcg) and frequency as your BPC157.”
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ForumReddit r/Peptides, thread ‘BPC-157 and TB4 dosage for female.’ (2024) (opens in a new tab)
“300-500mcg once daily of each peptide is a typical dosing regimen.”
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ForumMESO-Rx forum, ‘A comprehensive tb500 frag thread’ (2025) (opens in a new tab)
“I started with 125mcg twice a day and currently on “high” dosing, which is 750mcg twice a day.”
In blends with BPC-157 (Wolverine), GHK-Cu (GLOW) or KPV (KLOW)
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
- Forum: Reddit r/Peptides, thread ‘For those who take KPV’ (2026)
- Forum: Reddit r/Peptides, thread ‘BPC-157 and TB4 dosage for female.’ (2024)
- Practitioner: Jay Campbell, peptide author and coach (jaycampbell.com), GLOW protocol
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- Typically about 300–540 mcg of TB4 a day inside the blend (for example 2,000 mcg GHK-Cu + 320 mcg BPC-157 + 320 mcg TB4 per shot)
- How often
- Once or twice a day
- Route
- Injection under the skin
- Mixing
- Common blend vials: BPC-157/TB-500 5 mg + 5 mg or 10 mg + 10 mg; GLOW is GHK-Cu, TB-500 and BPC-157 in a 5:1:1 ratio (50 mg + 10 mg + 10 mg)
- Blend vials rarely say whether the ‘TB-500’ inside is the full protein or the fragment.
Sources (3)
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ForumReddit r/Peptides, thread ‘For those who take KPV’ (2026) (opens in a new tab)
“540mcg each of BPC/TB/KPV”
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ForumReddit r/Peptides, thread ‘BPC-157 and TB4 dosage for female.’ (2024) (opens in a new tab)
“18.75 doses of 2000mcg/320/320mcg”
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PractitionerJay Campbell, peptide author and coach (jaycampbell.com), GLOW protocol (opens in a new tab)
“You have the option to subcutaneously inject each of the three peptides individually, so long as you adhere to the 5:1:1 / GHK-Cu:TB-500:BPC-157 ratio.”
Every other day
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
- Forum: Reddit r/Peptides, thread ‘tb500/tb4 dosing help plse’ (2024)
- Forum: Reddit r/Peptides, thread ‘Full frag TB500 (TB4) version tanking my blood pressure’ (2023)
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- 2–2.5 mg
- How often
- Every other day
- Route
- Injection under the skin
- How long
- For example 4 weeks
Sources (2)
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ForumReddit r/Peptides, thread ‘tb500/tb4 dosing help plse’ (2024) (opens in a new tab)
“I’m currently testing 2.5mg every other day. I’ve done 1mg everyday and I’ve 500mcg 2x daily.”
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“initially did 2mg EOD for 4 weeks, and now tried upping my dose to 2mg ED after a 1-week break.”
Where the numbers come from
The name ‘TB-500’ comes from a veterinary product sold for racehorses and greyhounds around 2011, which doping labs identified as the 17–23 fragment. Human users adopted the name, but forum testers say most research-vial ‘TB-500’ is full-length thymosin beta-4. The twice-weekly 2–2.5 mg schedule is repeated across forums and vendor guides without a documented source. The daily 450–750 mcg schedule comes from compounding pharmacy catalogs (Tailor Made Compounding, 2019) and was copied onto clinic handouts; the American Academy of Anti-Aging Medicine monograph gives 300 mcg upward. None of these doses has been tested in people.
Home-made sprays and serums
Home-made nasal spray. Community recipes. Not tested.
Original source recordsHide
TB-500 is sold as a ready-made nasal spray (alone or with BPC-157), and at least one user made their own with saline. Users doubt it absorbs. Community only.
Best evidence: Community only (Reddit r/Peptides, 2023–2026); one vendor formula described by a buyer.
Vendor TB-500 nasal spray formula (Science.bio, as described by a buyer)
Original source-specific report. Collection checked 30 Sep 2026; presentation annotated 2026-10-01. Source date and reporter identity are not separately verified. Exact form and route are limited to the source’s description.
Ingredients and methodHide the recipe
Ingredients
| Ingredient | Amount | Role |
|---|---|---|
| TB-500 | not stated | Active |
| Deionised water (type 2) | Liquid | |
| Acetate | Buffer | |
| Benzalkonium chloride (BKC) | Preservative |
- Preservative
- Benzalkonium chloride.
- The buyer added their own BPC-157 (dissolved in bacteriostatic water) to the half-used TB-500 bottle.
Not stated: how it’s used and the amount per dose.
Sources (1)
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ForumReddit r/Peptides, u/Janime1983 (post, 2023-11-03) (opens in a new tab)
“The TB nasal bottle has deionized water(type 2), acetate, & BKC for stability purposes.”
1 mL saline per 5 mg (home-made)
Original source-specific report. Collection checked 30 Sep 2026; presentation annotated 2026-10-01. Source date and reporter identity are not separately verified. Exact form and route are limited to the source’s description.
Batch: 1 mL per 5 mg
Ingredients and methodHide the recipe
Ingredients
| Ingredient | Amount | Role |
|---|---|---|
| TB-500 | 5 mg | Active |
| Saline | 1 mL | Liquid |
- Preservative
- None.
- Storage
- Fridge (no alcohol).
- Keeps for
- Use quickly.
- The same user gave this ratio for DSIP and said they had made a TB-500 spray this way.
Not stated: how it’s used and the amount per dose.
Sources (1)
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ForumReddit r/Peptides, u/Puzzleheaded_Sea6731 (comment, 2025-03-06) (opens in a new tab)
“And I did make a TB500 nasal spray this way and it worked.”
How the method was put together, step by step with its sources
Editorial synthesis and reported strength comparison
This synthesized method combines source records. It has not been validated for preparation, sterility, compatibility, absorption or storage. Select a reported strength to inspect the arithmetic; no strength is selected for you.
No amounts were found in the reviewed recipe records for this one. What was found is under “Original source records”.
The general method for home-made nasal sprays, with its sources
Good to know
- Bacteriostatic water contains 0.9–1.1% benzyl alcohol as a preservative. Its label covers mixing injections, not nasal use; EU labels warn of allergic reactions.
- Some use store-bought saline nasal spray for its preservatives: often benzalkonium chloride, which can irritate the nose with long use, and sometimes benzyl alcohol.
- Never tap water: it is not sterile, and CDC researchers warn against it for nasal rinsing.
What people report
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User reports
Users doubt TB-500 is absorbed through the nose (‘The TB probably won’t really absorb much at all’).
Source: Reddit r/Peptides, u/Majalisk (2025-10-16) (opens in a new tab)
On the skin: home-made serums. Community recipes. Not tested.
Original source recordsHide
Thymosin beta-4 has been tested as a prescription-style skin gel (RGN-137) in phase 2 trials for leg ulcers, pressure ulcers and a blistering skin disease, at 0.01–0.1%. Community topical use is rare: people ask about putting TB-500 on the skin after derma-rolling, and others doubt it gets through the skin.
Best evidence: Phase 2 clinical trials of a thymosin beta-4 gel (RegeneRx, RGN-137) at 0.01%, 0.02%, 0.03% and 0.1%, once a day for up to 56–84 days. A 2010 report suggested 0.03% might speed healing of venous ulcers; a review says healing was about a month faster in patients who healed. The gel is not on the market.
Clinical-trial gel (RGN-137): 0.01%, 0.03% or 0.1% thymosin beta-4, once a day
Original source-specific report. Collection checked 30 Sep 2026; presentation annotated 2026-10-01. Source date and reporter identity are not separately verified. Exact form and route are limited to the source’s description.
- Study: ClinicalTrials.gov NCT00832091: Thymosin Beta 4 in Patients With Venous Stasis Ulcers
- Study: ClinicalTrials.gov NCT00311766: Thymosin Beta 4 in Epidermolysis Bullosa
- Study: ClinicalTrials.gov NCT00382174: Thymosin Beta 4 in Patients With Pressure Ulcers
- Study: Guarnera G et al., Ann N Y Acad Sci 2010
- Review: Treadwell T et al., Ann N Y Acad Sci 2012 (review)
Batch: not stated
Ingredients and methodHide the recipe
Ingredients
| Ingredient | % | Role |
|---|---|---|
| Thymosin beta-4 | 0.01%, 0.03% or 0.1% w/w (0.02% in the pressure-ulcer trial) | Active |
| Gel base (not described) | Base |
Method
Made by the trial sponsor; not available to buy.
- How it’s used
- Applied to the wound once a day for up to 84 days (venous and pressure ulcers) or 56 days (epidermolysis bullosa).
- The epidermolysis bullosa trial was terminated.
- These are wound treatments tested under medical supervision, not cosmetic recipes.
Sources (5)
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“0.01% weight/weight (w/w), 0.03% w/w, and 0.1% w/w thymosin beta 4 gel applied once daily for up to 84 days”
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StudyClinicalTrials.gov NCT00311766: Thymosin Beta 4 in Epidermolysis Bullosa (opens in a new tab)
“Topical Administration of 0.01%, 0.03%, and 0.1% Thymosin Beta 4 gel once a day (qd) up to 56 days”
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“3 doses of thymosin beta 4: 0.01% w/w, 0.02% w/w, and 0.1% w/w, administered topically once daily for up to 84 days”
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StudyGuarnera G et al., Ann N Y Acad Sci 2010 (opens in a new tab)
“a Tbeta4 dose of 0.03% may have the potential to accelerate wound healing”
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ReviewTreadwell T et al., Ann N Y Acad Sci 2012 (review) (opens in a new tab)
“in two phase 2 clinical trials of stasis and pressure ulcers, it was found to accelerate healing by almost a month in those patients that did heal.”
TB-500 after derma-rolling (community question, no recipe)
Original source-specific report. Collection checked 30 Sep 2026; presentation annotated 2026-10-01. Source date and reporter identity are not separately verified. Exact form and route are limited to the source’s description.
- Forum: Reddit r/Peptides, u/_Davesnothereman post (Feb 2024)
- Forum: Reddit r/Peptides, u/Majalisk (Feb 2024)
- Forum: Reddit r/Peptides, u/Difficult-Status-318 post (Jun 2025)
- Forum: Reddit r/Peptides, u/waaaaaardds (Sep 2021)
- Forum: Reddit r/Peptides, u/yyuqqi (May 2022)
Batch: not stated
Ingredients and methodHide the recipe
Ingredients
| Ingredient | Amount | Role |
|---|---|---|
| TB-500 (TB4), reconstituted | not stated | Active |
Method
No method was given; the poster asked what strength to use.
- How it’s used
- Proposed after derma-rolling.
- Another user listed TB-500 among freeze-dried peptides they wanted to combine in one serum with Snap-8, hyaluronic acid, BPC-157, NAD+ and glutathione, without amounts.
- A 2021 thread discussed a mouse study of TB-500 applied to the skin for hair growth; replies were sceptical that it works in people. One user put a BPC-157 and TB-500 cream on a hamstring strain.
Sources (5)
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ForumReddit r/Peptides, u/_Davesnothereman post (Feb 2024) (opens in a new tab)
“Going to make a topical tb-500 that I can put on after dermarolling…. What would be the dosage for that? .25mg concentration?”
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ForumReddit r/Peptides, u/Majalisk (Feb 2024) (opens in a new tab)
“No, I’m talking about its molecular weight being too high for topical absorption”
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ForumReddit r/Peptides, u/Difficult-Status-318 post (Jun 2025) (opens in a new tab)
“Snap 8 Hyaluronic Acid TB 500 BPC 157 NAD+ Glutathione”
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ForumReddit r/Peptides, u/waaaaaardds (Sep 2021) (opens in a new tab)
“If it worked, it would already be heavily used in the hair loss community.”
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ForumReddit r/Peptides, u/yyuqqi (May 2022) (opens in a new tab)
“I used a bpc 157 and tb 500 cream onto my hamstring strain and it healed in like 4 days.”
How the method was put together, step by step with its sources
Editorial synthesis and reported strength comparison
This synthesized method combines source records. It has not been validated for preparation, sterility, compatibility, absorption or storage. Select a reported strength to inspect the arithmetic; no strength is selected for you.
No amounts were found in the reviewed recipe records for this one. What was found is under “Original source records”.
The general method for home-made serums, with its sources
Good to know
- Use distilled water, not tap: tap water carries minerals, chlorine and microbes that can spoil the serum.
What people report
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User reports
Users doubt TB-500 is absorbed through the skin because the molecule is large.
Source: Reddit r/Peptides, u/Majalisk (Feb 2024) (opens in a new tab)
Research notes
Naming is the main problem. FDA and doping labs define ‘TB-500’ as the acetylated 17–23 fragment, but forum users sharing lab results (from the testing lab Janoshik, on MESO-Rx and Reddit) say most ‘TB-500’ vials contain full-length thymosin beta-4. Reported ‘TB-500’ doses therefore mostly apply here, and sources rarely say which molecule they mean. Sources directly contradict each other on schedule (2–2.5 mg twice a week versus 250–750 mcg daily) and on which form lasts longer in the body. The American Academy of Anti-Aging Medicine range ‘300 mcg – 1 gram daily’ looks like a typo but is repeated verbatim by users. The pharmacy behind the daily compounded schedule (Tailor Made Compounding) pleaded guilty in 2020 to distributing other unapproved peptides.
On the skin
The trial gel is thymosin beta-4 itself; many vendors sell ‘TB-500’, which may mean the full peptide or the 17–23 fragment. No home-made topical recipe with amounts was found.
What it is. In plain English.
The full-length thymosin beta-4 protein, studied in animals and in investigational eye-drop formulations; no injectable use is FDA-approved.
Thymosin beta-4 is a 43-amino-acid protein found inside many cells. Investigational RGN-259 eye drops contain thymosin beta-4; those studies do not establish injectable use.
Laboratory and animal studies suggest thymosin beta-4 can bind actin and may affect cell movement, wound repair and inflammation. These findings do not establish effects from injectable products; human studies have mainly examined investigational eye drops.
Reviewed 2026-10-01.
Structure. A close relative’s structure.
Related structure
Human thymosin beta 4 (indigo, amino acids 5–40) wrapped around actin (gray), in a crystal of an engineered protein in which thymosin beta 4 is joined to yeast actin by a short linker. A second copy of that protein is not drawn.
How it differs. This is the real thymosin beta 4 sequence, but as part of an engineered fusion: a 14-amino-acid linker joins it to the end of yeast actin, so the two form one chain. Amino acids 1–4 and 41–43 have no coordinates.
4PL7, Xue et al., 2014. Rendered with Mol* (Sehnal et al., 2021) from RCSB PDB data (Berman et al., 2000).
Specs. The facts at a glance.
- Length
- 43 amino acids
- Sold as
- TB4 10 mg
- In blends
- KLOW Blend80 mg per vial
- Glow Blend70 mg per vial
- BPC-157 / TB45 mg / 5 mg per vial
- BPC-157 / TB4 (double)10 mg / 10 mg per vial
- Deadpool Blend10 / 10 / 10 mg per vial
- Regulatory status
- Not approved. Investigational as eye drops. Banned by .
- Also known as
- TB4; often sold as “TB-500”
Sources. What this page rests on.
Every source behind this page, with what it says and when it was checked, is in the Source library.
Thymosin Beta-4 in the Source library
Original overview and research notes · annotated 2026-10-01
Historical collection text. Read alongside the claim corrections above; identity or evidence qualifications may supersede this wording.
Wound healing and recovery
A natural protein fragment that helps cells move into and repair wounds.
One of the most abundant peptides inside cells, especially platelets and white blood cells, and released at sites of injury.
It holds onto actin, a building block of the cell skeleton, controlling how cells reshape themselves. That helps cells migrate into wounds, supports and reduces inflammation and scarring.
Strong animal data for skin, eye, heart and muscle repair. Human trials of TB4 eye drops (RGN-259) for dry eye and corneal damage had mixed results. No injectable use has been tested to approval.
Original status record: Not approved. Investigational as eye drops. Banned by WADA.