Wolverine stack (BPC-157 + TB4/TB-500, separate vials)
The standard version is BPC-157 250–500 mcg a day plus TB4 (sold as TB-500) 2–2.5 mg twice a week for 4–6 weeks, then 2–2.5 mg once a week.
More about this
Some users take small daily TB4 doses instead. A nasal-spray version is also sold.
Loading, then maintenance
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
- Clinic: Beverly Hills Rejuvenation Center (clinic): BPC-157 and TB-500 ‘Wolverine’ dosage guide
- Practitioner: IMed University (training for medical providers): BPC-157 and TB-500 protocol guide
- Clinic: Denver Regenerative Medicine (clinic): Wolverine peptide stack guide
- Vendor: PSPeptides (vendor): Wolverine stack dosage guide
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- BPC-157: 250–500 mcg a day (often 250 mcg twice a day). TB4: 2–2.5 mg twice a week (4–5 mg a week) while loading, then 2–2.5 mg once a week.
- How often
- BPC-157 once or twice a day. TB4 twice a week, then once a week.
- Route
- Injection under the skin; BPC-157 is often injected near the injury
- How long
- Loading 4–6 weeks, then maintenance for about 4–8 more weeks
- Cycle
- One clinic cycles BPC-157 4–8 weeks on, 4 weeks off
- Ramp
- Front-loaded TB4 (loading phase), then a lower maintenance rate
- Mixing
- Separate vials. Example from a vendor guide: 5 mg BPC-157 + 2.5 mL water (250 mcg = 12.5 units); 5 mg TB4 + 2 mL water (2.5 mg = 100 units).
- Calculated (vendor example): 5 mg in 2.5 mL = 2 mg/mL, so 250 mcg is 0.125 mL (12.5 units). 5 mg in 2 mL = 2.5 mg/mL, so 2.5 mg is 1 mL (a full 100-unit syringe).
- A 1:1 pre-mixed vial cannot match this pattern, because TB4 is dosed in milligrams a few times a week and BPC-157 in micrograms daily.
Sources (4)
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“BPC-157 is dosed at roughly 250–500 mcg per day by subcutaneous injection, while TB-500 uses a two-phase schedule: a loading dose of about 2–2.5 mg twice weekly”
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PractitionerIMed University (training for medical providers): BPC-157 and TB-500 protocol guide (opens in a new tab)
“The loading phase generally lasts 4 to 6 weeks, with 2 to 2.5 mg administered subcutaneously twice weekly, for a total of 4 to 5 mg per week.”
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ClinicDenver Regenerative Medicine (clinic): Wolverine peptide stack guide (opens in a new tab)
“BPC-157: Daily or twice-daily injections, often cycled 4–8 weeks on, 4 weeks off”
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VendorPSPeptides (vendor): Wolverine stack dosage guide (opens in a new tab)
“For BPC-157, research protocols commonly reference doses in the range of 250-500mcg per administration, once or twice daily.”
Forum variants on the TB4 schedule
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
- Forum: Reddit r/Peptides, /u/Tim3k- (Jun 2023)
- Forum: Reddit r/Peptides, /u/bennyballsback (Jun 2025)
- Forum: Reddit r/Peptides, /u/Apprehensive-Leek392 (Jun 2025)
- Forum: Reddit r/Peptides, /u/dung-beetle-ZA (Mar 2025)
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- BPC-157 250–500 mcg once or twice a day, with TB4 anywhere from 1 mg twice a week to 5 mg every 5 days, or small TB4 doses every day
- How often
- BPC-157 daily; TB4 every few days, weekly, or daily
- Route
- Injection under the skin (belly)
- How long
- About 8 weeks
- Cycle
- Then 1–2 months off (one user’s advice)
- Users disagree on whether BPC-157 needs cycling; one cites a practitioner saying it does not, while TB4 should be cycled.
Sources (4)
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ForumReddit r/Peptides, /u/Tim3k- (Jun 2023) (opens in a new tab)
“I pin 5mg TB500 every 5 days. I pin .5mg BPC157 twice a day.”
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ForumReddit r/Peptides, /u/bennyballsback (Jun 2025) (opens in a new tab)
“BPC157 @ 500mcg daily for 8 weeks TB500 @ 2mg twice per week for 8 weeks You should stop after those two months for at least a month or two.”
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ForumReddit r/Peptides, /u/Apprehensive-Leek392 (Jun 2025) (opens in a new tab)
“I did TB 1mg 2x a week for first two weeks to lead and then 1mg 1x per week. For 8 weeks. Bpc 2x daily”
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ForumReddit r/Peptides, /u/dung-beetle-ZA (Mar 2025) (opens in a new tab)
“I’m busy micro dosing both tb500 once a day and bpc twice a day am/pm”
Show all 3 protocolsShow fewer protocols
Nasal spray (pre-mixed BPC-157 + TB-500)
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
- Vendor: Peptidist / Gorilla Healing (vendor): Wolverine Nasal Spray product page
- Vendor: Peptidist / Gorilla Healing (vendor): customer review on the product page
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- 50 mcg per spray (standard) or 100 mcg per spray (‘Ultra’), as sold. Sprays per day are not stated on the product page.
- How often
- Daily
- Route
- Nasal spray
- How long
- One reviewer used it for about 4 weeks, then planned a week off
- Mixing
- Sold ready-made: 10 mg in 10 mL (100 sprays)
- Calculated 10 mg in 10 mL = 1 mg/mL, so a 50 mcg spray is about 0.05 mL. The label does not say how the 50 mcg splits between BPC-157 and TB-500.
Sources (2)
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VendorPeptidist / Gorilla Healing (vendor): Wolverine Nasal Spray product page (opens in a new tab)
“NORMAL IS 50MCG PER SPRAY ULTRA HAS 100MCG PER SPRAY”
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VendorPeptidist / Gorilla Healing (vendor): customer review on the product page (opens in a new tab)
“I’ve been using it for about 4 weeks now I’m going to take a week off”
Where the numbers come from
The name refers to the Marvel character’s fast healing. The pairing spread through bodybuilding and biohacking forums and vendor marketing, and later through regenerative clinics. The doses are forum and clinic conventions (BPC-157 in micrograms daily, TB4 in milligrams with a loading phase), not results from any human trial of the pair.
Cautions
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Regulator
The FDA says it has little or no human safety information for BPC-157, and no human exposure data for the TB4 fragment sold as TB-500. It cites immune-reaction and impurity concerns for both.
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User reports
Clinic-reported side effects of TB4 in this stack: mild tiredness in the first weeks, a brief head rush right after injecting, and injection-site discomfort.
Source: Perfect B (clinic, Doral FL): Wolverine peptide stack page (opens in a new tab)
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User reports
Journalists found users reporting whole-body itching, intense anxiety, and loss of pleasure (anhedonia) with BPC-157.
Source: STAT News: ‘BPC-157: the peptide with big claims and scant evidence’ (Feb 2026) (opens in a new tab)
Research notes
Vendors sell ‘TB-500’ as either full thymosin beta-4 or the short 17–23 fragment, and labels do not always say which; the FDA’s concerns name the fragment. In July 2026 an FDA advisory panel voted to recommend letting pharmacies compound BPC-157 and TB-500; the FDA had not decided at the time of writing.