Blends · Peptide Field Book

80 mg per vial

KLOW Blend

GHK-Cu50 mg
BPC-15710 mg
Thymosin Beta-410 mg
KPV10 mg

Collected rationale, not demonstrated combination efficacy. Ingredient findings do not validate this mixture.

The Glow blend plus KPV. It combines the two repair peptides, GHK-Cu’s skin and collagen effects, and KPV’s anti-inflammatory action.

Community protocols. Reported online. Not clinically validated.

Community reports Collection date: 30 Sep 2026

Most users mix the 80 mg vial with 3 mL of water and inject about 12 units a day (about 2 mg GHK-Cu and 400 mcg each of BPC-157, TB4 and KPV).

More about this

Reported doses run from about 10 to 15 units, daily or 5 days a week, for 4–12 weeks. One clinic uses 5 days a week for 8 weeks.

Not testedFrequently encountered in reviewed sources

Daily dose keyed to 2 mg GHK-Cu (3 mL mix)

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: 10–12 units of a vial mixed with 3 mL. That is about 1.7–2 mg GHK-Cu plus 330–400 mcg each of BPC-157, TB4 and KPV. 12 units (2 mg GHK-Cu) is the figure quoted most.
How often: Once a day, or 5 days on and 2 days off

Route: Injection under the skin (belly or buttock)
How long: Commonly 4–8 weeks; one user ran 2 vials (about 50 days)
Cycle: Then about 3 weeks off. Some run it continuously and only break when life gets in the way.
Mixing: 80 mg vial (50/10/10/10) + 3 mL bacteriostatic water

Calculated 50 mg GHK-Cu + 10 mg BPC-157 + 10 mg TB4 + 10 mg KPV = 80 mg in 3 mL = 26.67 mg/mL of blend. One syringe unit is 0.01 mL, so it holds 167 mcg GHK-Cu + 33.3 mcg BPC-157 + 33.3 mcg TB4 + 33.3 mcg KPV. 10 units (0.10 mL) = 1.67 mg GHK-Cu + 333 mcg BPC-157 + 333 mcg TB4 + 333 mcg KPV; 12 units (0.12 mL) = 2 mg GHK-Cu + 400 mcg BPC-157 + 400 mcg TB4 + 400 mcg KPV; 15 units (0.15 mL) = 2.5 mg GHK-Cu + 500 mcg BPC-157 + 500 mcg TB4 + 500 mcg KPV.
At 12 units a day one vial lasts about 25 days. At 10 units 5 days a week it lasts about a month.
KPV, BPC-157 and TB4 are fixed at one-fifth of the GHK-Cu, so users cannot adjust them separately. Some users dislike this and buy the parts separately.

Sources (4)

ForumReddit r/Peptidesource, /u/Doctordup2 (Jun 2025) (opens in a new tab)

“50/10/10/10 3mL bac 2mg/400mcg/400mcg/400mcg”
ForumReddit r/Peptidesource, /u/s_lamoureux (Jul 2025) (opens in a new tab)

“If you have a 50mg/10mg/10mg/10mg vial of KLOW and mix with 3ml of bac water, you will get: 2mg/400mcg/400mcg/400mcg dose with 12 units.”
ForumReddit r/Peptides, /u/Spartan-of-Now (Jan 2026) (opens in a new tab)

“3ml of BAC water, 12 units daily!”
ForumReddit r/Peptidesource, /u/Bobbyaxelrod112334 (Jun 2025) (opens in a new tab)

“The 5 on 2 off a lot of people do is to use 3ml bac then 10ius 5/2 will last a month. I did 12ius daily till gone”

Not testedCommon

Higher daily dose (about 2.5 mg GHK-Cu)

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: About 2.5 mg GHK-Cu plus 500 mcg each of BPC-157, TB4 and KPV (15 units of a 3 mL mix)
How often: Once a day, or on weekdays only

Route: Injection under the skin
How long: 8 weeks straight is common
Cycle: One user: 40 days on, 20 days off
Mixing: 80 mg vial + 3 mL bacteriostatic water

Calculated 50 mg GHK-Cu + 10 mg BPC-157 + 10 mg TB4 + 10 mg KPV = 80 mg in 3 mL = 26.67 mg/mL of blend. One syringe unit is 0.01 mL, so it holds 167 mcg GHK-Cu + 33.3 mcg BPC-157 + 33.3 mcg TB4 + 33.3 mcg KPV. 15 units (0.15 mL) = 2.5 mg GHK-Cu + 500 mcg BPC-157 + 500 mcg TB4 + 500 mcg KPV.
Other users replied that 2.5 mg is above the usual 1–2 mg of GHK-Cu.

Sources (4)

ForumReddit r/Peptides, /u/Few-Addendum8636 (Dec 2025) (opens in a new tab)

“I started a daily 2.5/.5/.5/.5 dose this week.”
ForumReddit r/Peptidesource, /u/True_Plate7264 (Jul 2025) (opens in a new tab)

“Daily i would pin 15 units . That is a daily dose of 2500mcg cu, 500mcg tb, 500mcg 157, 500mcg kpv.”
ForumReddit r/Peptides, /u/grlymax (Dec 2025) (opens in a new tab)

“I run it at 7 days a week for 8 weeks.”
ForumReddit r/Peptides, /u/T_he_panda (Dec 2025) (opens in a new tab)

“8 do this dosage 40 days on 20 days off”

Show all 5 protocolsShow fewer protocols

Not testedSometimes seen

Loading phase, then maintenance

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: Weeks 1–4: 10 units of a 2 mL mix (4 mg of blend: 2.5 mg GHK-Cu + 500 mcg each of the others). Weeks 5–12: 5 units (half that). One Reddit user loaded at 8 mg of blend for 2 weeks, then 4 mg.
How often: 5 days a week while loading, then 3 days a week

Route: Injection under the skin
Timing: Morning (guide site)
How long: 8–12 weeks
Cycle: Then 4–8 weeks off
Ramp: Front-loaded, then stepped down
Mixing: 80 mg vial + 2 mL bacteriostatic water (guide site). The Reddit user did not give a mix.

Calculated (2 mL mix): 50 mg GHK-Cu + 10 mg BPC-157 + 10 mg TB4 + 10 mg KPV = 80 mg in 2 mL = 40.00 mg/mL of blend. One syringe unit is 0.01 mL, so it holds 250 mcg GHK-Cu + 50 mcg BPC-157 + 50 mcg TB4 + 50 mcg KPV. 5 units (0.05 mL) = 1.25 mg GHK-Cu + 250 mcg BPC-157 + 250 mcg TB4 + 250 mcg KPV; 10 units (0.10 mL) = 2.5 mg GHK-Cu + 500 mcg BPC-157 + 500 mcg TB4 + 500 mcg KPV.
Calculated for the Reddit user’s figures: 8 mg of blend = 5 mg GHK-Cu + 1 mg each of BPC-157, TB4 and KPV. 4 mg of blend = 2.5 mg GHK-Cu + 500 mcg each.
A guide site describes a variant that keeps 10 units of a 3 mL mix but steps the frequency down: daily for weeks 1–4, 5 times a week for weeks 5–8, then 2–3 times a week.

Sources (4)

VendorThe Peptide Catalog: KLOW dosing guide (affiliate site that lists vendor discount codes) (opens in a new tab)

“Loading dose 10 units on insulin syringe (4 mg total blend), 5x/week — weeks 1-4”
VendorThe Peptide Catalog: KLOW dosing guide (affiliate site that lists vendor discount codes) (opens in a new tab)

“Maintenance dose 5 units (2 mg total blend), 3x/week — weeks 5-12”
ForumReddit r/Peptidesource, /u/Active_Growth_112 (Jun 2025) (opens in a new tab)

“my protocal is a 2 week loading phase of 8mg 5 on 2 off. Then drop to 4mg for 10 weeks with 5 on 2 off.”
ReviewDosage Peptide: KLOW 80 mg dosage guide (information site) (opens in a new tab)

“The documented research schedule runs daily through weeks 1–4, five times a week through weeks 5–8, then two to three times a week for maintenance.”

Not testedSometimes seen

Same 2 mg target, different water volume

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: About 2 mg GHK-Cu a day, drawn as 10 units of a 2.5 mL mix; one user uses 20 units of a 6 mL mix (about 1.7 mg GHK-Cu)
How often: Once a day

Route: Injection under the skin
How long: 30 days per vial; 2–3 vials
Cycle: Then 1–2 months off
Mixing: 80 mg vial + 2.5 mL, or + 6 mL (the 6 mL mix needs a larger vial than the one supplied)

Calculated (2.5 mL mix): 50 mg GHK-Cu + 10 mg BPC-157 + 10 mg TB4 + 10 mg KPV = 80 mg in 2.5 mL = 32.00 mg/mL of blend. One syringe unit is 0.01 mL, so it holds 200 mcg GHK-Cu + 40 mcg BPC-157 + 40 mcg TB4 + 40 mcg KPV. 10 units (0.10 mL) = 2 mg GHK-Cu + 400 mcg BPC-157 + 400 mcg TB4 + 400 mcg KPV.
Calculated (6 mL mix): 50 mg GHK-Cu + 10 mg BPC-157 + 10 mg TB4 + 10 mg KPV = 80 mg in 6 mL = 13.33 mg/mL of blend. One syringe unit is 0.01 mL, so it holds 83.3 mcg GHK-Cu + 16.7 mcg BPC-157 + 16.7 mcg TB4 + 16.7 mcg KPV. 20 units (0.20 mL) = 1.67 mg GHK-Cu + 333 mcg BPC-157 + 333 mcg TB4 + 333 mcg KPV.
Users say more water means less sting. Less water means fewer units to draw.

Sources (2)

ForumReddit r/Peptides, /u/larkspur82 (Jan 2026) (opens in a new tab)

“When you want a 2mg dose, you can either add 2.5mL for easy dose units or you can add 3mL for less sting.”
ForumReddit r/Peptides, /u/Separate_Surround513 (May 2026) (opens in a new tab)

“6ml BW. 20IU per day for 30 days. 2 or 3 vials (2-3 months) then take a month or two off.”

Not testedSometimes seen

Clinic course (pharmacy-compounded KLOW)

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Clinic: EllieMD (telehealth clinic): KLOW Injection product page

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: Not published. The prescriber sets the dose. Supplied as two ready-mixed 5 mL vials meant to last about 2 months.
How often: 5 times a week

Route: Injection under the skin
How long: About 8 weeks
Cycle: Then a 2-week break before repeating
Mixing: Arrives pre-mixed from the pharmacy (no home mixing)

The clinic does not publish its concentration, so the amount per injection cannot be worked out.

Sources (1)

ClinicEllieMD (telehealth clinic): KLOW Injection product page (opens in a new tab)

“A commonly used protocol involves administration five times per week for approximately eight weeks, followed by a two-week break before repeating the cycle, if appropriate.”

Where the numbers come from

KLOW is the GLOW blend with KPV added; the ‘K’ stands for KPV. A Reddit user who says they wrote the original ‘Anela Protocol’ for GHK-Cu calls KLOW an adaptation of it. By that account the BPC-157-to-GHK-Cu ratio was chosen to stop GHK-Cu stinging, and TB4 and KPV were later added at the same amount as BPC-157. That is an unverified self-report. Doses are keyed to about 2 mg GHK-Cu a day, not to any study.

Cautions

User reports
Users report the same injection sting as with GLOW, which they put down to the copper in GHK-Cu. GLOW users also report welts, bruising and tiredness (see the GLOW entry).
Source: Reddit r/Peptides thread ‘KLOW injection’ (Jul 2025) (opens in a new tab)
Regulator
The FDA says it has found no human exposure data for KPV by any route and does not know whether it would cause harm. It lists immune-reaction and impurity concerns for injected GHK-Cu and says it has little or no human safety data for BPC-157 and TB-500.
Source: FDA: Bulk drug substances that may present significant safety risks (page updated April 22, 2026) (opens in a new tab)
Regulator
In July 2026 an FDA advisory panel voted 8–6 to recommend allowing pharmacies to compound BPC-157, KPV and TB-500, over the objections of FDA career scientists. The scientists cited missing evidence, safety concerns, and uncertainty about what the peptides chemically are. The vote is advice only, and nothing was approved.
Source: NBC News: FDA panel recommends easing some peptide restrictions (July 2026) (opens in a new tab)

Show all 4 cautionsShow fewer cautions

Study
No study, human or animal, has tested the four peptides together at any ratio.
Source: Regen Wellness (Dallas clinic): ‘KLOW Peptide Blend: what the research actually shows’ (opens in a new tab)

Research notes

Most KLOW is 50/10/10/10 like this vendor’s, but users also report 40/10/10/10 vials, so unit counts do not transfer between sellers. One information site calls the same 80 mg blend the ‘Deadpool Stack’, a name other vendors use for different blends. One user says they and others take up to 5 mg GHK-Cu a day without problems; others call anything above 2 mg overkill. The loading protocol’s numbers come from an affiliate guide site, not a named user. The clinic product (EllieMD) is pharmacy-compounded and pre-mixed at an unpublished strength. Some users think a fixed 5:1:1:1 ratio doses KPV poorly and prefer separate vials. Regulatory status changed in 2026 (see cautions), so the site’s status text for BPC-157 and KPV may need a review.

70 mg per vial

Glow Blend

Collected rationale, not demonstrated combination efficacy. Ingredient findings do not validate this mixture.

Marketed for skin quality and recovery. GHK-Cu is the only ingredient with meaningful human skin data, and that data is for topical use.

Community protocols. Reported online. Not clinically validated.

Community reports Collection date: 30 Sep 2026

Most users dose this blend by its GHK-Cu content. The usual pattern is to mix the 70 mg vial with 3 mL of water and inject 10–12 units once a day (about 1.7–2 mg GHK-Cu with 330–400 mcg each of BPC-157 and TB4).

More about this

Reported doses run from about 6 to 15 units, daily or 5 days a week, in runs of 4–8 weeks.

Not testedFrequently encountered in reviewed sources

Daily skin and repair dose (3 mL mix)

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: 9–15 units of a vial mixed with 3 mL. That is about 1.5–2.5 mg GHK-Cu plus 300–500 mcg each of BPC-157 and TB4. 10–12 units is the figure quoted most.
How often: Once a day, or 5 days on and 2 days off

Route: Injection under the skin (belly or upper buttock)
Timing: Morning or bedtime. People move it depending on whether it makes them sleepy or alert.
How long: Usually 4–8 weeks
Cycle: Often 2–4 weeks off after a run. Some users never break.
Ramp: Some start around 6 units and work up to 10–12 units.
Mixing: 70 mg vial (50/10/10) + 3 mL bacteriostatic water

Calculated 50 mg GHK-Cu + 10 mg BPC-157 + 10 mg TB4 = 70 mg in 3 mL = 23.33 mg/mL of blend. One syringe unit is 0.01 mL, so it holds 167 mcg GHK-Cu + 33.3 mcg BPC-157 + 33.3 mcg TB4. 9 units (0.09 mL) = 1.5 mg GHK-Cu + 300 mcg BPC-157 + 300 mcg TB4; 10 units (0.10 mL) = 1.67 mg GHK-Cu + 333 mcg BPC-157 + 333 mcg TB4; 12 units (0.12 mL) = 2 mg GHK-Cu + 400 mcg BPC-157 + 400 mcg TB4; 15 units (0.15 mL) = 2.5 mg GHK-Cu + 500 mcg BPC-157 + 500 mcg TB4.
People pick the dose by the GHK-Cu amount (commonly 1.5–2 mg a day). BPC-157 and TB4 simply come along at the fixed 5:1:1 ratio.
Tricks reported for the sting: extra bacteriostatic water drawn into the syringe, a longer needle, fatty injection sites, and splitting the dose into small nearby injections.

Sources (4)

ForumGLP1 Forum thread ‘Glow Peptide’ (user mixing Anela’s 50/10/10 ratio into 3 mL) (opens in a new tab)

“A dose containing 0.300mg BPC-157, 0.300 TB-500, and 1.5mg GHK-Cu daily before bedtime works well for me. You obtain that with a DRAW of 9 (nine) units”
ForumReddit r/Peptides, /u/Bobbyaxelrod112334 (Jun 2025) (opens in a new tab)

“Use 3mls of bac and then 12 units daily will get u 2mg 400mcg 400mcg”
ForumReddit r/Peptides, /u/ParcelPosted (Dec 2024) (opens in a new tab)

“I have a 50/10/10 blend and use 3 ML of Bac - 12 or 15 units a day.”
ForumReddit r/Peptides, deleted user (Feb 2025) (opens in a new tab)

“I use 50/10/10 and pin 12u mon-Fri.”

Not testedCommon

Higher daily dose (about 2.5 mg GHK-Cu)

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Forum: Reddit r/Peptides, /u/johnsondough (May 2025)
Forum: Reddit r/Peptides, /u/johnsondough (May 2025)
Forum: Reddit r/Peptides, /u/creep1352 (Mar 2025)
Forum: Reddit r/Peptides, /u/Pedro_uk (Jun 2025)

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: About 2.5 mg GHK-Cu plus 500 mcg each of BPC-157 and TB4. That is 10 units of a 2 mL mix, or 15 units of a 3 mL mix.
How often: Once a day, or 5 days on and 2 days off

Route: Injection under the skin
Timing: Morning or bedtime
How long: About 6 weeks; one vial lasts about 20 days at this dose
Cycle: One user planned 6 weeks on, 3 weeks off
Mixing: 70 mg vial + 2 mL or 3 mL bacteriostatic water

Calculated (2 mL mix): 50 mg GHK-Cu + 10 mg BPC-157 + 10 mg TB4 = 70 mg in 2 mL = 35.00 mg/mL of blend. One syringe unit is 0.01 mL, so it holds 250 mcg GHK-Cu + 50 mcg BPC-157 + 50 mcg TB4. 10 units (0.10 mL) = 2.5 mg GHK-Cu + 500 mcg BPC-157 + 500 mcg TB4.
Calculated (3 mL mix): 50 mg GHK-Cu + 10 mg BPC-157 + 10 mg TB4 = 70 mg in 3 mL = 23.33 mg/mL of blend. One syringe unit is 0.01 mL, so it holds 167 mcg GHK-Cu + 33.3 mcg BPC-157 + 33.3 mcg TB4. 15 units (0.15 mL) = 2.5 mg GHK-Cu + 500 mcg BPC-157 + 500 mcg TB4.
Users on this dose were the ones most often asking about afternoon tiredness. Others replied that 2.5 mg of GHK-Cu is above the usual 1–2 mg.

Sources (4)

ForumReddit r/Peptides, /u/johnsondough (May 2025) (opens in a new tab)

“started taking a GLOW (50/10/10) blend protocol at a dose of 2,500mcg of GHK-Cu and 500mcg of BPC-157 and TB-500 per day (5 on/2 off)”
ForumReddit r/Peptides, /u/johnsondough (May 2025) (opens in a new tab)

“2mL of BAC water and pulling up 10 units (0.1cc)”
ForumReddit r/Peptides, /u/creep1352 (Mar 2025) (opens in a new tab)

“I’ve started taking GLOW 50/10/10 reconstituted with 3ml bac, 15 units (2.5mg/500/500mcg) as soon as I wake up”
ForumReddit r/Peptides, /u/Pedro_uk (Jun 2025) (opens in a new tab)

“I’m currently taking one dose per day, consisting of 500 mcg BPC, 500 mcg TB, and 2.5 mg GHK-CU. So each vial does me 20 days or so”

Show all 4 protocolsShow fewer protocols

Not testedSometimes seen

Smaller shots twice a day

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: About 1 mg GHK-Cu per shot (about 6 units of a 3 mL mix), twice a day
How often: Twice a day

Route: Injection under the skin
How long: About 6 weeks
Cycle: 2–4 weeks off
Mixing: 3 mL bacteriostatic water per vial

The idea is that BPC-157 is short-lived, so splitting keeps levels steadier. Others say once a day is good enough.
Calculated (the 45.1/12.6/6.9 mg vial this advice was given for): 45.1 mg GHK-Cu + 12.6 mg TB4 + 6.9 mg BPC-157 = 64.6 mg in 3 mL = 21.53 mg/mL of blend. One syringe unit is 0.01 mL, so it holds 150 mcg GHK-Cu + 42 mcg TB4 + 23 mcg BPC-157. 6 units (0.06 mL) = 902 mcg GHK-Cu + 252 mcg TB4 + 138 mcg BPC-157.
Calculated (the same 3 mL and 6 units with this vendor’s 50/10/10 vial): 50 mg GHK-Cu + 10 mg BPC-157 + 10 mg TB4 = 70 mg in 3 mL = 23.33 mg/mL of blend. One syringe unit is 0.01 mL, so it holds 167 mcg GHK-Cu + 33.3 mcg BPC-157 + 33.3 mcg TB4. 6 units (0.06 mL) = 1 mg GHK-Cu + 200 mcg BPC-157 + 200 mcg TB4.

Sources (2)

ForumReddit r/Peptides, /u/badger0136 (Dec 2024) (opens in a new tab)

“Typical is 1mg ghk per dose, twice a day. Which would then be .25 TB and about .15 BPC. 3 ml of water would keep dose to 6 units.”
ForumReddit r/Peptides, /u/Content_Rooster_6318 (Jun 2025) (opens in a new tab)

“He wanted to accelerate repair so he did twice a day for a few weeks and was still within the dose ranges.”

Not testedSometimes seen

Clinic course (pharmacy-compounded GLOW)

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: Not published. The prescriber sets the dose.
How often: Not stated on the clinic pages

Route: Injection under the skin
How long: 4–8 weeks. One clinic recommends 8 weeks; another says 4–6 weeks.
Cycle: One clinic: a 4-week break before another cycle
Mixing: Supplied by the clinic

These clinic pages sell the same three peptides as a program but do not publish the amount per injection.

Sources (2)

ClinicBetter Med Spa, Chicago: ‘Repair & Glow’ peptide therapy page (opens in a new tab)

“A standard Repair & Glow cycle runs 4 to 8 weeks. We recommend 8 weeks for most patients, followed by a 4-week break before starting another cycle if desired.”
ClinicFlorida Surgery & Weight Loss Center: GLOW Blend peptide therapy page (opens in a new tab)

“Florida Surgery & Weight Loss Center offers a GLOW Blend Peptide Program for $439. The typical treatment length is 4–6 weeks based on provider recommendations and patient needs.”

Where the numbers come from

Community-built. Dosing is keyed to GHK-Cu (about 1.5–2 mg a day), and BPC-157 and TB4 ride along at a 5:1:1 ratio. Users credit the 50/10/10 recipe to ‘Anela’, an online peptide protocol writer. She says her first protocol was a ‘painless’ injected GHK-Cu mix with BPC-157, and that clinics later used her protocols without credit. That is her own account. Vendors and clinics then sold the mix as ‘GLOW’. Earlier vials were 35/10/5 (50 mg).

Cautions

User reports
Stinging or burning at the injection site is the most common complaint. Users blame the GHK-Cu and describe a wasp-sting feeling lasting hours to days.
Source: Reddit r/Peptides thread ‘Glow that doesn’t sting?’ (opens in a new tab)
User reports
Red welts or lumps at the injection site. One user said GHK-Cu welts lasted 3–6 months.
Source: Reddit r/Peptides thread ‘GLOW (GHK-Cu/TB500/BCP157) site reaction’ (opens in a new tab)
User reports
Quarter-sized bruises after injections, especially in the first days.
Source: Reddit r/Peptides thread ‘Bruising with GLOW’ (opens in a new tab)

Show all 8 cautionsShow fewer cautions

User reports
Heavy tiredness or sleepiness a few hours after a dose. Users usually blame the TB4 and move the dose to bedtime.
Source: Reddit r/Peptides thread ‘Fatigue from GLOW’ (opens in a new tab)
User reports
The opposite also happens: some users feel wired and cannot sleep when they dose at night.
Source: Reddit r/Peptides thread ‘Glow Protocol’ (Feb 2025) (opens in a new tab)
User reports
The author of the Anela Protocols says she is receiving adverse skin reports from people in their 20s on GHK-Cu. She will not recommend injected GHK-Cu for anyone under 25 and would not go above 2 mg.
Source: Krysia’s Substack: ‘Ask Anela’ Q&A with the author of the Anela Protocols (Sep 2026) (opens in a new tab)
Regulator
The FDA says injected GHK-Cu may cause immune reactions because of aggregation and impurities. It has little or no human safety information for BPC-157 and no human exposure data for the TB4 fragment sold as TB-500.
Source: FDA: Bulk drug substances that may present significant safety risks (page updated April 22, 2026) (opens in a new tab)
Study
No published study has tested the three peptides together. The human evidence for GHK-Cu is for creams on the skin, not injections. With injected copper there is no set upper limit; one clinic says people with Wilson disease or liver problems should avoid it.
Source: Beverly Hills Rejuvenation Center: ‘The Glow Protocol: what the evidence actually shows’ (opens in a new tab)

Research notes

Vial recipes differ by seller (35/10/5, 50/10/10, 50/12/8, 45.1/12.6/6.9 and 27/5/10 mg all appear in these threads), so the same number of units delivers different amounts. This vendor’s 50/10/10 matches the most common current recipe. Users disagree on daily use versus 5 days on and 2 days off, and on whether to split doses. One user reported taking a higher-BPC/TB4 homemade mix daily for 18 months without cycling. Regulatory status moved in 2026. The FDA’s safety-risk page (April 2026) now files BPC-157, injectable GHK-Cu and the TB4 fragment under ‘nominated but withdrawn’ instead of category 2, while still describing their risks. In July 2026 an FDA advisory panel voted to recommend letting pharmacies compound BPC-157 and TB-500 (GHK-Cu was not on the agenda). Nothing is FDA-approved, and the FDA’s final decision is pending. The site’s status text for these peptides may need a review.

10 mg per vial

BPC-157 / TB4

BPC-1575 mg
Thymosin Beta-45 mg

Collected rationale, not demonstrated combination efficacy. Ingredient findings do not validate this mixture.

The most common repair stack, often nicknamed “Wolverine.” The idea is that BPC-157’s blood-vessel effects and TB4’s cell-migration effects complement each other. Only animal data exist for each.

Community protocols. Reported online. Not clinically validated.

Community reports Collection date: 30 Sep 2026

Users dose the 10 mg vial by total blend, most often mixing it with 2 mL of water and injecting 10 units (250 mcg of each peptide) once or twice a day.

More about this

Some go up to 500 mcg of each a day. Common schedules are daily or weekdays only, for several weeks to a few months (some take 1 month off after 2 months on).

Not testedFrequently encountered in reviewed sources

250–500 mcg of each a day, split into one or two shots

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: 10 units of a 2 mL mix = 500 mcg of blend (250 mcg of each), once or twice a day. Some take 500 mcg of each a day, split in two.
How often: Once or twice a day; some skip weekends

Route: Injection under the skin (belly, or near the injury)
How long: Several weeks to a few months
Cycle: Weekends off, or about 2 months on and 1 month off
Mixing: 10 mg vial (5 mg + 5 mg) + 2 mL bacteriostatic water

Calculated 5 mg BPC-157 + 5 mg TB4 = 10 mg in 2 mL = 5.00 mg/mL of blend. One syringe unit is 0.01 mL, so it holds 25 mcg BPC-157 + 25 mcg TB4. 10 units (0.10 mL) = 250 mcg BPC-157 + 250 mcg TB4; 20 units (0.20 mL) = 500 mcg BPC-157 + 500 mcg TB4.
A common mix-up: users confuse 500 mcg of blend with 500 mcg of each peptide. In a 1:1 vial, 500 mcg of blend is 250 mcg of each.

Sources (4)

ForumReddit r/Peptides, /u/nunswithknives (Oct 2024) (opens in a new tab)

“I’ve reconstituted my BPC/TB 5mg/5mg blend with two ml BAC. I was dosing with 500mcg twice a day (10 units)”
ForumReddit r/Peptides, /u/AcidicMountaingoat (Oct 2024) (opens in a new tab)

“That mix and amount is giving you 250mcg of EACH peptide.”
ForumReddit r/Peptides, /u/Popular_Spinach_9288 (Jun 2025) (opens in a new tab)

“500mcg of BPC and 500mcg of TB daily split into two doses each day.”
ForumReddit r/Peptides, /u/TechnoViking01 (Jun 2025) (opens in a new tab)

“Yeah I’ve run it Mon to Fri with weekends off for several months at a time another common practice is 2 months on 1 month off”

Not testedSometimes seen

Clinic ‘Wolverine Blend’ course

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: Not published. The provider sets the units and adjusts them month to month.
How often: Once a day, 5 days on and 2 days off

Route: Injection under the skin (belly, rotating sites)
Timing: Before bedtime, at least 2 hours after the last meal
How long: 1-month cycles of 20 injections; up to 3 in a row
Cycle: Then a 2-month break
Mixing: Blend vial + 2 mL bacteriostatic water

The clinic does not say how much of each peptide its vial holds, so its units cannot be converted. The same page also says to use separate syringes for each peptide, which contradicts the single-vial description.

Sources (2)

ClinicPerfect B (clinic, Doral FL): Wolverine peptide stack page (opens in a new tab)

“The Wolverine Blend arrives as a single vial containing both peptides, and it is injected once a day for 5 days followed by 2 days off.”
ClinicPerfect B (clinic, Doral FL): Wolverine peptide stack page (protocol table) (opens in a new tab)

“2 mL of bacteriostatic water, swirled not shaken, refrigerated after mixing”

Show all 3 protocolsShow fewer protocols

Not testedSometimes seen

Step-up over 8 weeks (guide-site calculation)

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Review: PeptideDosages (information site): BPC-157 + TB-500 10 mg blend chart

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: With a 3 mL mix: 15 units (500 mcg of blend) in weeks 1–2, 20 units in weeks 3–4, then 30 units (1 mg of blend: 500 mcg of each) in weeks 5–8
How often: Once a day

Route: Injection under the skin
How long: 4–8 weeks
Ramp: Raised every 2 weeks
Mixing: 10 mg vial + 3 mL bacteriostatic water

Calculated 5 mg BPC-157 + 5 mg TB4 = 10 mg in 3 mL = 3.33 mg/mL of blend. One syringe unit is 0.01 mL, so it holds 16.7 mcg BPC-157 + 16.7 mcg TB4. 15 units (0.15 mL) = 250 mcg BPC-157 + 250 mcg TB4; 20 units (0.20 mL) = 333 mcg BPC-157 + 333 mcg TB4; 30 units (0.30 mL) = 500 mcg BPC-157 + 500 mcg TB4.
This schedule is a guide site’s calculation ‘based on community-reported use’, not a named user’s protocol.

Sources (1)

ReviewPeptideDosages (information site): BPC-157 + TB-500 10 mg blend chart (opens in a new tab)

“Calculation row: 500 mcg total blend contains 250 mcg BPC-157 plus 250 mcg TB-500 and measures 15 U-100 units (0.15 mL) .”

Where the numbers come from

A pre-mixed version of the ‘Wolverine’ stack (BPC-157 + TB4). Separate-vial protocols dose BPC-157 in micrograms daily and TB4 in milligrams a few times a week; the 1:1 vial forces a compromise, and most users simply dose the blend like BPC-157.

Cautions

Regulator
The FDA says it has little or no human safety information for BPC-157 and no human exposure data for the TB4 fragment sold as TB-500. It flags possible immune reactions and impurities for both.
Source: FDA: Bulk drug substances that may present significant safety risks (page updated April 22, 2026) (opens in a new tab)
Regulator
In July 2026 an FDA advisory panel voted 8–6 to recommend allowing pharmacies to compound BPC-157 and TB-500, over the objections of FDA scientists who cited missing evidence and safety concerns. This was advice only; nothing was approved.
Source: NBC News: FDA panel recommends easing some peptide restrictions (July 2026) (opens in a new tab)
User reports
Users describe tiredness with TB4-containing blends, especially in the first weeks.
Source: Reddit r/Peptides thread ‘Fatigue from GLOW’ (opens in a new tab)

Show all 5 cautionsShow fewer cautions

User reports
Some users link BPC-157 to anhedonia (loss of pleasure), mostly in people also taking antidepressant or anti-anxiety medicines. These are anecdotes.
Source: Reddit r/Peptides thread ‘Taking 500 daily of each BPC and TB in a blend’ (Jun 2025) (opens in a new tab)
User reports
Journalists found Reddit users reporting whole-body itching, intense anxiety and anhedonia with BPC-157.
Source: STAT News: ‘BPC-157: the peptide with big claims and scant evidence’ (Feb 2026) (opens in a new tab)

Research notes

A 1:1 vial cannot reproduce the usual separate-vial pattern (TB4 at 2–2.5 mg twice a week). Dosing for BPC-157 gives far less TB4 than that pattern; dosing for TB4 gives very large BPC-157 doses. Users also say premixes can’t be adjusted and some prefer separate vials. The clinic source does not state its vial strength. Many ‘TB-500’ products are the short fragment, which the FDA says has no human exposure data.

20 mg per vial

BPC-157 / TB4 (double)

BPC-15710 mg
Thymosin Beta-410 mg

Collected rationale, not demonstrated combination efficacy. Ingredient findings do not validate this mixture.

The same combination at twice the amount per vial.

Community protocols. Reported online. Not clinically validated.

Community reports Collection date: 30 Sep 2026

Users of the 20 mg vial commonly mix it with 2 mL of water, so 5 units = 500 mcg of blend (250 mcg of each), and take 500 mcg to 1 mg of blend once or twice a day.

More about this

Some go up to 1 mg of each a day for injuries. Runs are usually 4–8 weeks.

Not testedFrequently encountered in reviewed sources

500 mcg to 1 mg of blend a day (2 mL mix)

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: 5–10 units of a 2 mL mix = 500 mcg to 1 mg of blend (250–500 mcg of each), once a day, or 500 mcg of blend twice a day
How often: Once or twice a day

Route: Injection under the skin (belly, or near the injury)
How long: About 8 weeks (2 vials at 500 mcg a day)
Cycle: Users say to stop after 8–10 weeks
Mixing: 20 mg vial (10 mg + 10 mg) + 2 mL bacteriostatic water

Calculated 10 mg BPC-157 + 10 mg TB4 = 20 mg in 2 mL = 10.00 mg/mL of blend. One syringe unit is 0.01 mL, so it holds 50 mcg BPC-157 + 50 mcg TB4. 5 units (0.05 mL) = 250 mcg BPC-157 + 250 mcg TB4; 10 units (0.10 mL) = 500 mcg BPC-157 + 500 mcg TB4.
At 500 mcg of blend a day, one vial lasts about 40 days.

Sources (3)

VendorThe Peptide Catalog: Wolverine 20 mg reconstitution guide (affiliate site that lists vendor discount codes) (opens in a new tab)

“2mL is the most-cited dilution. It produces clean, round numbers for every common dose — 5 units for 500mcg, 7.5 units for 750mcg.”
ForumReddit r/Peptides, /u/mandzhalas (May 2025) (opens in a new tab)

“So far I have used up 10mg over the course of 10 days, injecting 500mcg twice a day.”
ForumReddit r/Peptides, /u/YogurtclosetNo9608 (May 2025) (opens in a new tab)

“You want to stick with 500mcg each twice daily for acute injuries and 250mcg twice daily for maintenance”

Not testedSometimes seen

High dose for injuries (1 mg of each a day)

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Forum: Reddit r/Peptides, /u/MajorFish04 (May 2025)

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: 1,000 mcg of each peptide a day
How often: Daily

Route: Injection under the skin, near the injured joints
How long: 3 weeks reported

Other users in the same thread argued that more is not better and that 200–500 mcg a day is enough.

Sources (1)

ForumReddit r/Peptides, /u/MajorFish04 (May 2025) (opens in a new tab)

“I’ve been on 1000 mcg of each for 3 weeks straight between my knee and shoulder.”

Show all 3 protocolsShow fewer protocols

Not testedSometimes seen

Clinic ‘Wolverine Blend’ course

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: Not published. The provider sets the units and adjusts them month to month.
How often: Once a day, 5 days on and 2 days off

Route: Injection under the skin (belly, rotating sites)
Timing: Before bedtime, at least 2 hours after the last meal
How long: 1-month cycles of 20 injections; up to 3 in a row
Cycle: Then a 2-month break
Mixing: Blend vial + 2 mL bacteriostatic water

The clinic does not state its vial strength, so its units cannot be converted.

Sources (2)

ClinicPerfect B (clinic, Doral FL): Wolverine peptide stack page (opens in a new tab)

“The Wolverine Blend arrives as a single vial containing both peptides, and it is injected once a day for 5 days followed by 2 days off.”
ClinicPerfect B (clinic, Doral FL): Wolverine peptide stack page (protocol table) (opens in a new tab)

“2 mL of bacteriostatic water, swirled not shaken, refrigerated after mixing”

Where the numbers come from

The same ‘Wolverine’ pairing as the 5 mg/5 mg vial at double strength. Dosing is by total blend, anchored on the usual BPC-157 range of 250–500 mcg a day.

Cautions

Regulator
The FDA says it has little or no human safety information for BPC-157 and no human exposure data for the TB4 fragment sold as TB-500. It flags possible immune reactions and impurities for both.
Source: FDA: Bulk drug substances that may present significant safety risks (page updated April 22, 2026) (opens in a new tab)
Regulator
In July 2026 an FDA advisory panel voted 8–6 to recommend allowing pharmacies to compound BPC-157 and TB-500, over the objections of FDA scientists who cited missing evidence and safety concerns. This was advice only; nothing was approved.
Source: NBC News: FDA panel recommends easing some peptide restrictions (July 2026) (opens in a new tab)
User reports
Users describe tiredness with TB4-containing blends, especially in the first weeks.
Source: Reddit r/Peptides thread ‘Fatigue from GLOW’ (opens in a new tab)

Show all 5 cautionsShow fewer cautions

User reports
Some users link BPC-157 to anhedonia (loss of pleasure), mostly in people also taking antidepressant or anti-anxiety medicines. These are anecdotes.
Source: Reddit r/Peptides thread ‘Taking 500 daily of each BPC and TB in a blend’ (Jun 2025) (opens in a new tab)
User reports
Journalists found Reddit users reporting whole-body itching, intense anxiety and anhedonia with BPC-157.
Source: STAT News: ‘BPC-157: the peptide with big claims and scant evidence’ (Feb 2026) (opens in a new tab)

Research notes

Same ratio problem as the 5/5 vial: 1:1 blends give much less TB4 than the separate-vial loading pattern. One user reported 1 mg of each daily; others pushed back. The 2 mL mixing figure comes from an affiliate guide site; the Reddit posts did not all state their water volume.

30 mg per vial

Deadpool Blend

Collected rationale, not demonstrated combination efficacy. Ingredient findings do not validate this mixture.

The repair stack plus Cartalax, a bioregulator claimed to target cartilage. Aimed at joint injuries.

Community protocols. Reported online. Not clinically validated.

Community reports Collection date: 30 Sep 2026

No community protocol found for a BPC-157 + TB4 + Cartalax vial. Vendors that sell it (10/10/10 mg, 5/5/10 mg or 40 mg versions) say they cannot give dosing advice.

More about this

The ‘Deadpool’ name is also used for several other, different products and stacks, whose protocols do not apply here.

Where the numbers come from

A vendor naming convention: ‘Deadpool’ follows ‘Wolverine’ (BPC-157 + TB4), with Cartalax added as a claimed cartilage bioregulator. Several vendors carry it under this name.

Cautions

Regulator
The FDA says it has little or no human safety information for BPC-157 and none for the TB4 fragment sold as TB-500. Cartalax is not an approved drug anywhere.
Source: FDA: Bulk drug substances that may present significant safety risks (page updated April 22, 2026) (opens in a new tab)

Research notes

‘Deadpool’ means at least four different things: (1) this BPC-157/TB4/Cartalax vial (sold 10/10/10 by several US vendors, 5/5/10 by VPeptide, and 40 mg by ION Peptide); (2) a South African ‘Deadpool Stack’ pen of TB-500 10 mg + BPC-157 5 mg + KPV 5 mg, labelled 1–2 mg five times a week for 2–4 weeks; (3) one information site’s name for the KLOW 80 mg blend; (4) a five-peptide separate-vial stack posted on Reddit (see stacks). A Reddit user also called GHK-Cu + KPV ‘the Deadpool stack’. None of these protocols can be carried over to this vial. Cartalax dosing on its own appears on guide sites (a few mg a day) but was not researched here.

10 mg per vial

CJC-1295 no DAC / Ipamorelin

CJC-1295 (no DAC)5 mg
Ipamorelin5 mg

Collected rationale, not demonstrated combination efficacy. Ingredient findings do not validate this mixture.

Pairs a signal with a signal. They act on different pituitary receptors, and together produce a larger growth-hormone pulse than either alone.

Community protocols. Reported online. Not clinically validated.

Community reports Collection date: 30 Sep 2026

The most common protocol is about 100 mcg of each peptide once a day at bedtime, on an empty stomach, 5 days on and 2 days off. Clinics often step up to 200–300 mcg of each.

More about this

With the 10 mg vial mixed with 2 mL, 100 mcg of each is 4 units. An older forum protocol uses 100 mcg of each 2–3 times a day. Runs are usually 8–16 weeks.

Not testedFrequently encountered in reviewed sources

Bedtime dose, 5 days a week

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: 100 mcg of each peptide to start, often raised to 200 mcg (sometimes 300 mcg) of each. With a 2 mL mix: 4 units = 100 mcg of each, 8 units = 200 mcg of each.
How often: Once a day, 5 days on and 2 days off (weekends off)

Route: Injection under the skin (belly)
Timing: At bedtime, on an empty stomach (about 2–3 hours after eating)
How long: 8–12 weeks; some clinics run 12–16 weeks
Cycle: Then 4–6 weeks off
Ramp: 100 mcg of each for about 4 weeks, then 200 mcg of each
Mixing: 10 mg vial (5 mg + 5 mg) + 2 mL bacteriostatic water

Calculated 5 mg CJC-1295 + 5 mg ipamorelin = 10 mg in 2 mL = 5.00 mg/mL of blend. One syringe unit is 0.01 mL, so it holds 25 mcg CJC-1295 + 25 mcg ipamorelin. 4 units (0.04 mL) = 100 mcg CJC-1295 + 100 mcg ipamorelin; 8 units (0.08 mL) = 200 mcg CJC-1295 + 200 mcg ipamorelin; 12 units (0.12 mL) = 300 mcg CJC-1295 + 300 mcg ipamorelin.
Watch the wording. One clinic points out that ‘200 mcg’ can mean 200 mcg of the blend (100 mcg of each) or 200 mcg of each (400 mcg in total).
One clinic scales the dose by body weight: about 100–150 mcg of each under 150 lb, and up to 300 mcg of each over 250 lb.

Sources (4)

ClinicAlpha Rejuvenation (clinic): CJC-1295 Ipamorelin dosage page (opens in a new tab)

“CJC-1295 (no DAC) : 100 mcg before bed Ipamorelin : 100 mcg before bed Frequency : Once daily, 5 days per week”
ClinicBeverly Hills Rejuvenation Center (clinic): CJC-1295/Ipamorelin dosage guide (opens in a new tab)

“100 mcg of each peptide per dose , injected subcutaneously once daily at bedtime on an empty stomach, five days per week.”
ClinicBeverly Hills Rejuvenation Center (clinic): mixing example for a 5 mg/5 mg vial in 2 mL (opens in a new tab)

“5,000 mcg ÷ (2 mL × 100) = 25 mcg per unit of each peptide”
ClinicThin Works (clinic): CJC-1295 Ipamorelin dosage guide (opens in a new tab)

“The protocol we describe for this therapy is 200 mcg by subcutaneous injection just before bedtime, five days per week, with two off days.”

Not testedCommon

Two or three shots a day (‘saturation dose’)

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: 100 mcg of each per shot (4 units of a 2 mL mix); some use 150 mcg of each (6 units) twice a day
How often: 2–3 times a day: on waking, before training, and at bedtime

Route: Injection under the skin
Timing: On an empty stomach, away from meals; the bedtime shot is treated as the most important
How long: Often open-ended; one user said he would run it indefinitely
Mixing: 5 mg + 5 mg vial + 2 mL bacteriostatic water

Calculated 5 mg CJC-1295 + 5 mg ipamorelin = 10 mg in 2 mL = 5.00 mg/mL of blend. One syringe unit is 0.01 mL, so it holds 25 mcg CJC-1295 + 25 mcg ipamorelin. 4 units (0.04 mL) = 100 mcg CJC-1295 + 100 mcg ipamorelin; 6 units (0.06 mL) = 150 mcg CJC-1295 + 150 mcg ipamorelin.
This follows the bodybuilding-forum ‘saturation dose’ idea: about 1 mcg per kg, or 100 mcg, per shot, with more shots rather than bigger ones.
One user posted that his IGF-1 (a blood marker of growth hormone activity) rose from 227 to 406 ng/mL after 2 weeks on this schedule. That is a single self-report.

Sources (4)

ForumAnabolicMinds forum, user tregar (May 2022): 100 mcg Mod GRF + 100 mcg ipamorelin 3 times a day (opens in a new tab)

“5mg mod grf 1-29 (cjc-1295 no DAC) + 5mg ipamorelin (100 mcg of each) x 3 times a day, 1st thing in morning”
ForumAnabolicMinds forum, user tregar (May 2022): mixing and units (opens in a new tab)

“add 2ml bac water in vial 4iu (2 small tics, as each tic = 2iu) on a standard 100iu (1ml) insulin pin gives 100mcg of each”
ForumSuperiorMuscle forum: ‘Peptides basics by author Datbtrue’ (Aug 2010 repost) (opens in a new tab)

“You also want to dose PWO and in the morning. That is 3 times a day.”
ForumReddit r/Peptides, /u/analog_potatoes (Jan 2023) (opens in a new tab)

“with 2ML BAC in 5/5 blend, you’re pinning 6units on U-100 to get 150 mcg of each peptide. This is exactly what I do twice daily.”

Show all 3 protocolsShow fewer protocols

Not testedSometimes seen

Clinic step-up to 1 mg of blend

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: 6 units of a 2 mL mix (0.3 mg of blend) in week 1, rising 2 units a week to 20 units (1.0 mg of blend) by the end of month 2, then held there
How often: Once a day, 5 days on and 2 days off

Route: Injection under the skin (belly)
Timing: Before bed, at least 2 hours after the last meal
How long: 1-month cycles of 20 injections; up to 3 cycles in a row
Cycle: Then a 2-month break. Later cycles restart at the full 20 units.
Ramp: +2 units each week: 6, 8, 10, 12, then 14, 16, 18, 20 units
Mixing: Blend vial + 2 mL bacteriostatic water

Calculated the clinic’s figures (6 units = 0.3 mg) mean 5 mg/mL of blend, which fits a 10 mg vial in 2 mL. If that vial is 5 mg + 5 mg like this vendor’s, 6 units = 150 mcg of each and 20 units = 500 mcg of each. The clinic does not state the split.
The top dose here is higher than most other sources, which stay at 100–300 mcg of each.

Sources (2)

ClinicPerfect B (clinic, Doral FL): CJC-1295/Ipamorelin dosage protocol (opens in a new tab)

“Starting cjc 1295 ipamorelin dosage: 6 units (0.3 mg) daily in week one, escalating to 20 units (1.0 mg) by month two.”
ClinicPerfect B (clinic, Doral FL): cycle length (opens in a new tab)

“A cycle is one month and 20 injections. Up to 3 cycles run back to back, and then a 2 month break before repeating.”

Where the numbers come from

Pairing a mimic of growth-hormone-releasing hormone with a ghrelin mimic, and the ‘100 mcg saturation dose’, come from bodybuilding forums, especially guides by the writer ‘Datbtrue’ (reposted around 2010), which drew on 1990s growth-hormone secretagogue studies. Anti-aging and men’s-health clinics later settled on one bedtime shot, 5 days a week. The 1:1 pre-mixed 5 mg/5 mg vial became the default format, which is why 100 mcg of each is the usual starting dose.

Cautions

Regulator
The FDA says it has identified serious adverse events with CJC-1295, including a faster heart rate and a whole-body blood-vessel-widening (flushing) reaction. It says clinical data are limited.
Source: FDA: Bulk drug substances that may present significant safety risks (page updated April 22, 2026) (opens in a new tab)
Regulator
For ipamorelin the FDA cites a published study with serious adverse events, including death, when it was given into a vein for gut motility. It says it has no safety information for certain other injection routes. Ipamorelin is still on its category 2 list for outsourcing facilities.
Source: FDA: Bulk drug substances that may present significant safety risks (page updated April 22, 2026) (opens in a new tab)
User reports
A clinic lists what its patients report: mild water retention, temporary tiredness and vivid dreams at standard doses. At higher doses it lists hunger, headaches and joint aches.
Source: Alpha Rejuvenation (clinic): CJC-1295 Ipamorelin dosage page (opens in a new tab)

Research notes

Doses are often written ambiguously (‘200 mcg’ of the blend versus of each peptide), which can double or halve the real amount. One Florida clinic’s top dose (20 units, about 1 mg of blend a day) is well above other sources. The forum ‘saturation dose’ logic comes from 1990s secretagogue research, not from any trial of this pair. The FDA page still lists ipamorelin in category 2 for outsourcing facilities; CJC-1295 moved to ‘nominated but withdrawn’.

13 mg per vial

Tesamorelin / Ipamorelin

Tesamorelin10 mg
Ipamorelin3 mg

Collected rationale, not demonstrated combination efficacy. Ingredient findings do not validate this mixture.

Same logic as CJC/Ipamorelin, using the approved GHRH drug tesamorelin as the GHRH half.

Community protocols. Reported online. Not clinically validated.

Community reports Collection date: 30 Sep 2026

Users dose tesamorelin/ipamorelin blends by the tesamorelin, usually about 1 mg a night on an empty stomach, often 5 nights a week. With this 10/3 vial mixed with 2 mL, that is 20 units (1 mg tesamorelin + 300 mcg ipamorelin).

More about this

Reported tesamorelin doses run from 300 mcg to 2 mg, and schedules from 8-week cycles to many months.

Not testedFrequently encountered in reviewed sources

About 1 mg tesamorelin at bedtime

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: About 1 mg tesamorelin, taking whatever ipamorelin comes with it. With this 10/3 vial in 2 mL, 20 units = 1 mg tesamorelin + 300 mcg ipamorelin. Users on other blends report 1 mg with 150 mcg ipamorelin.
How often: Once a day, often 5 days on and 2 days off

Route: Injection under the skin (belly, rotating sites)
Timing: In the evening before bed, on an empty stomach
How long: 8 weeks (guide site); some users run it for months
Cycle: 8 weeks on, 8 weeks off (guide site)
Mixing: 13 mg vial (10 mg + 3 mg) + 2 mL bacteriostatic water

Calculated 10 mg tesamorelin + 3 mg ipamorelin = 13 mg in 2 mL = 6.50 mg/mL of blend. One syringe unit is 0.01 mL, so it holds 50 mcg tesamorelin + 15 mcg ipamorelin. 10 units (0.10 mL) = 500 mcg tesamorelin + 150 mcg ipamorelin; 20 units (0.20 mL) = 1 mg tesamorelin + 300 mcg ipamorelin.
Users say to ‘dose it based on the highest concentration’, meaning the tesamorelin. One user reports that cycles under 500 mcg of tesamorelin were described as unsuccessful.
About 1 mg is roughly half the approved drug’s daily amount.

Sources (4)

VendorThe Peptide Catalog: tesamorelin + ipamorelin 10/3 blend guide (affiliate site that lists vendor discount codes) (opens in a new tab)

“For 1mg tesamorelin: 1,000 / 5,000 = 0.2 mL = 20 units. The ipamorelin at 3mg/2mL = 1.5mg/mL rides along at 300mcg per 20-unit dose”
VendorThe Peptide Catalog: tesamorelin + ipamorelin 10/3 blend guide (FAQ) (opens in a new tab)

“once daily in the evening before bed, 5 days on / 2 days off, for 8 weeks on / 8 weeks off”
ForumReddit r/Peptides, /u/Bryanole27 (Mar 2025) (opens in a new tab)

“Dose it based on the highest concentration, in this case, Tesa.”
ForumReddit r/Peptides, /u/OptionDeli (Mar 2025) (opens in a new tab)

“I take Tesa/Ipam 1mg/150mcg. I’d read enough reviews of successful cycles using 1mg and unsuccessful cycles of <500mcg”

Not testedSometimes seen

Up to 2 mg tesamorelin at night

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: 2 mg tesamorelin with 100–300 mcg ipamorelin at night. Some users say 1 mg did little for them. With this 10/3 vial, 2 mg of tesamorelin would be 40 units of a 2 mL mix.
How often: Once nightly

Route: Injection under the skin
Timing: At night, fasted
How long: About 8 weeks or longer

Calculated (this 10/3 vial in 2 mL): 10 mg tesamorelin + 3 mg ipamorelin = 13 mg in 2 mL = 6.50 mg/mL of blend. One syringe unit is 0.01 mL, so it holds 50 mcg tesamorelin + 15 mcg ipamorelin. 40 units (0.40 mL) = 2 mg tesamorelin + 600 mcg ipamorelin.
At 2 mg of tesamorelin this vial also delivers 600 mcg of ipamorelin, well above the 80–250 mcg users describe for ipamorelin. The forum users here took the two separately.

Sources (3)

ForumReddit r/Peptides, /u/Personal_Nebula_7100 (Jun 2025) (opens in a new tab)

“Later on I tried 2mg Tesa and 250mcg PM only and it was much better.”
ForumReddit r/Peptides, /u/Ok-Improvement-4526 (Jun 2025) (opens in a new tab)

“I did tesa 2mg at night with ipa 100-300 mcg also at night. Fasted. Great results.”
ForumReddit r/Peptides, /u/Both-Matter-2071 (Jun 2025) (opens in a new tab)

“Tesa dose is 1-2mg, Ipa dose is 80-250mcg.”

Show all 5 protocolsShow fewer protocols

Not testedSometimes seen

Vendor ‘experience level’ tiers

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Vendor: Ten23 Labs (vendor): Tesamorelin 10 mg + Ipamorelin 3 mg product page

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: Beginner 0.2–0.5 mg, intermediate 0.5–1 mg, advanced 1–2 mg a day
How often: Once a day (sometimes twice)

Route: Injection under the skin
Timing: Just before bed
How long: 4–12 weeks
Cycle: Breaks ‘as needed’

The vendor’s table is headed ‘Tesa / Ipa’ and does not say whether the mg figures mean tesamorelin or the whole blend. For this 10/3 vial that changes the dose by about 30%.

Sources (1)

VendorTen23 Labs (vendor): Tesamorelin 10 mg + Ipamorelin 3 mg product page (opens in a new tab)

“Beginner 0.2–0.5mg / day just before bed Intermediate 0.5–1.0mg / day just before bed Advanced 1.0–2.0mg / day just before bed”

Not testedSometimes seen

Low-dose prescribed pairing

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Forum: Reddit r/Peptides, /u/Professional_Buy_572 (Mar 2024)

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: 300 mcg tesamorelin + 150 mcg ipamorelin (15 units of the user’s prescribed vial)
How often: 6 nights a week

Route: Injection under the skin
Timing: Night, at least 4 hours after eating
How long: About 9 months

Calculated 15 units = 0.15 mL holding 450 mcg in total, so the user’s vial was 3 mg/mL (2 mg/mL tesamorelin + 1 mg/mL ipamorelin, a 2:1 ratio). This is a different ratio from this vendor’s 10/3 vial.

Sources (1)

ForumReddit r/Peptides, /u/Professional_Buy_572 (Mar 2024) (opens in a new tab)

“I’m prescribed 300mcg Tesamorelin + 150mcg Ipamorelin (15 units) subcutaneously. I used it for around 9 momths or so, six nights per week.”

Not testedSometimes seen

1 mg tesamorelin + 500 mcg ipamorelin nightly

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Forum: ExcelMale forum, user swoops36 (May 2020)

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: 1 mg tesamorelin + 500 mcg ipamorelin
How often: Once nightly

Route: Injection under the skin
Timing: Night
How long: 6+ weeks reported

Sources (1)

ForumExcelMale forum, user swoops36 (May 2020) (opens in a new tab)

“Just started my 6th week. 1mg Tesa + 500mcg Ipa.”

Where the numbers come from

Pairs tesamorelin, an approved drug that mimics growth-hormone-releasing hormone, with ipamorelin, following the CJC-1295/ipamorelin logic. Doses are anchored on tesamorelin, usually about half the approved daily amount. The 10/3 ratio keeps tesamorelin as the lead; vendors sell many other ratios.

Cautions

User reports
One user on a prescribed blend twice nearly passed out: dizzy, racing heart, sweating. He suspected low blood sugar, since both episodes came after little or no food. He was also fasting and using kratom, so the cause is unclear.
Source: Reddit r/Peptides thread ‘Tesamorelin/Ipamorelin side effects - nearly passed out 2x’ (Mar 2024) (opens in a new tab)
User reports
Carpal-tunnel-like hand pain, daytime sleepiness and feeling ‘pumped’ and vascular after about 3–5 weeks.
Source: ExcelMale forum, user swoops36 (May 2020) (opens in a new tab)
Regulator
For ipamorelin the FDA cites a published study with serious adverse events, including death, when it was given into a vein. Ipamorelin remains on its category 2 list for outsourcing facilities.
Source: FDA: Bulk drug substances that may present significant safety risks (page updated April 22, 2026) (opens in a new tab)

Research notes

Blends come in many ratios (10/3, 5/5, 10/10, 10/5, 8/4, 11/4, 2:1), so unit counts do not carry over between vendors. Users disagree on whether 1 mg or 2 mg of tesamorelin is needed; one cites a practitioner (Dr. Seeds) recommending 1 mg tesamorelin + 100 mcg ipamorelin morning and night. Users note that 1:1 blends push ipamorelin far above the usual 100–300 mcg once tesamorelin reaches 1–2 mg. The ‘1 mg + 300 mcg, 5 days on and 2 off, 8 weeks on and 8 off’ protocol comes from an affiliate guide site, not a named user. Tesamorelin is an approved drug, so its label risks also apply; those are covered in the verified section.

20 mg per vial

Selank / Semax

N-Acetyl Selank Amidate10 mg
N-Acetyl Semax Amidate10 mg

Collected rationale, not demonstrated combination efficacy. Ingredient findings do not validate this mixture.

A nootropic pairing: Selank for calm, Semax for focus. Both are Russian nasal medicines, sold here in their modified forms.

Community protocols. Reported online. Not clinically validated.

Community reports Collection date: 30 Sep 2026

Mostly used as a nasal spray. Forum users of the N-acetyl forms commonly take 1–2 sprays of each (about 200 mcg a spray) once or twice a day, roughly 600 mcg of Semax and 300 mcg of Selank a day, in 3–4 week runs.

More about this

Some inject 300–900 mcg a day. A 1:1 pre-mixed vial forces equal amounts of both, which does not match how most forum users dose them.

Not testedFrequently encountered in reviewed sources

Nasal spray, 1–2 sprays of each once or twice a day (forum)

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Forum: Reddit r/Nootropics, /u/SocialT (Jan 2016)
Forum: Reddit r/Nootropics, /u/baccheion (Jan 2016)
Forum: Reddit r/Nootropics, /u/SocialT (Jan 2016)
Forum: Reddit r/Nootropics, /u/insaiyan17 (Nov 2024)

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: N-acetyl Semax about 600 mcg a day (some up to 1,200 mcg); N-acetyl Selank about 300 mcg a day (some half that). Typically 1–2 sprays of each (about 200 mcg a spray), once or twice a day.
How often: Once or twice a day (morning and mid-afternoon)

Route: Nasal spray
Timing: Early in the day; Semax later in the day can disturb sleep
How long: 3–4 weeks on
Cycle: Then 1 week off; some do 3 weeks on, 3 weeks off
Ramp: Some add one spray every 5–10 minutes until they feel an effect, stopping at 1,200 mcg
Mixing: Forum users mostly bought ready-made sprays (100–300 mcg per spray); no mixing stated

These are separate Semax and Selank sprays. With this vendor’s 1:1 vial, every spray delivers equal amounts of both, so users cannot take twice as much Semax as Selank.

Sources (4)

ForumReddit r/Nootropics, /u/SocialT (Jan 2016) (opens in a new tab)

“Most people like to do 1-2 sprays of each, once or twice a day. I would start out at one spray of each, then go from there.”
ForumReddit r/Nootropics, /u/baccheion (Jan 2016) (opens in a new tab)

“N-Acetyl Semax is usually dosed at 600mcg, but some have to go up to 1200mcg to get results. N-Acetyl Selank is usually dosed at 300mcg, but some only need half.”
ForumReddit r/Nootropics, /u/SocialT (Jan 2016) (opens in a new tab)

“If you’re planning to do everyday I’d just do like 3-4 weeks on and 1 week off.”
ForumReddit r/Nootropics, /u/insaiyan17 (Nov 2024) (opens in a new tab)

“Common to take em in cycles together, so like 3 weeks on 3 weeks off”

Not testedCommon

Nasal spray (vendor and clinic figures)

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: About 125–250 mcg of each peptide per spray (vendor example), 1–3 sprays per nostril. Clinic ranges for the standard forms: Semax 200–600 mcg a day, Selank 250–500 mcg a day; the N-acetyl forms are said to need less.
How often: Once or twice a day

Route: Nasal spray
How long: Courses of about 2–4 weeks (vendor: 10–14 days in research)
Cycle: Then a break to reassess
Mixing: Vendor example: 5 mg/5 mg vial + 4 mL = 1.25 mg/mL of each, moved into a spray bottle

Calculated at 1.25 mg/mL of each, a 0.1 mL spray holds 125 mcg of each and a 0.2 mL spray 250 mcg of each. This vendor’s 10 mg/10 mg vial would need 8 mL to reach the same strength.
The vendor’s own numbers do not add up: 1–3 sprays per nostril at 125–250 mcg per spray is 250–1,500 mcg of each, not the 200–900 mcg it states.

Sources (4)

VendorProliven Labs (vendor): Selank/Semax 5 mg/5 mg blend product page (opens in a new tab)

“Reconstitute to 1.25mg/mL per peptide (e.g., 4mL solvent for 5mg/5mg vial).”
VendorProliven Labs (vendor): Selank/Semax 5 mg/5 mg blend product page (opens in a new tab)

“Typical research protocol: 1-3 sprays per nostril (200-900 mcg per peptide) once or twice daily.”
ClinicBeverly Hills Rejuvenation Center (clinic): Semax and Selank dosage and stacking guide (opens in a new tab)

“Semax (0.1% nasal spray): commonly referenced at 200–600 mcg/day for focus & clarity; N-Acetyl Semax Amidate is more potent, so referenced doses run lower.”
ClinicBeverly Hills Rejuvenation Center (clinic): Semax and Selank dosage and stacking guide (opens in a new tab)

“Course length: many protocols run Semax and Selank in courses of roughly 2–4 weeks , then take a break to reassess.”

Show all 5 protocolsShow fewer protocols

Not testedSometimes seen

Equal amounts of each, 500 mcg to 1 mg a day

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: 500 mcg of each a day (the same user also mentions 1 mg of each)
How often: Daily

Route: Nasal spray
How long: About 2 months reported

This is the pattern a 1:1 pre-mixed vial allows. The user also took P21, which is not on this site. The same user asked whether the peptides could be mixed in one spray; a reply said the worst outcome at high doses was headache and nausea.

Sources (2)

ForumReddit r/Peptides, /u/Odd-Negotiation5262 (Jun 2022) (opens in a new tab)

“I have been taking Semax and P21and Selank intranasal for 2 months. It is working pretty well.”
ForumReddit r/Peptides, /u/Odd-Negotiation5262 (Jun 2022) (opens in a new tab)

“I have been on 500 mcg of each per day.”

Not testedSometimes seen

Injection under the skin

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: 300–900 mcg a day, split into 1–2 doses (vendor: Semax 300–900 mcg, Selank 200–750 mcg). One user reports up to 1.5 mg twice a day.
How often: Once or twice a day

Route: Injection under the skin
How long: Courses of about 10–14 days to 4 weeks
Mixing: Vendor example: 5 mg/5 mg vial + 2 mL = 2.5 mg/mL of each

Calculated (vendor’s 5 mg/5 mg vial in 2 mL): 5 mg Selank + 5 mg Semax = 10 mg in 2 mL = 5.00 mg/mL of blend. One syringe unit is 0.01 mL, so it holds 25 mcg Selank + 25 mcg Semax. 10 units (0.10 mL) = 250 mcg Selank + 250 mcg Semax; 20 units (0.20 mL) = 500 mcg Selank + 500 mcg Semax.
Calculated this vendor’s 10 mg/10 mg vial would need 4 mL to reach the same 2.5 mg/mL of each.

Sources (4)

VendorProliven Labs (vendor): Selank/Semax 5 mg/5 mg blend product page (opens in a new tab)

“Reconstitute to a concentration of 2.5mg/mL per peptide (e.g., 2mL solvent for 5mg/5mg vial).”
VendorProliven Labs (vendor): Selank/Semax 5 mg/5 mg blend product page (opens in a new tab)

“Typical research doses: 300-900 mcg/day for Semax; 200-750 mcg/day for Selank, split into 1-2 administrations.”
ForumReddit r/Nootropics, /u/Amazing_Extension207 (Nov 2024) (opens in a new tab)

“Dose if injecting 300-900mcg a day. I’ve done as much as 1.5mg twice a day with amazing results.”
ForumReddit r/Nootropics, /u/dz93 (Apr 2015) (opens in a new tab)

“I usually take NA Semax in the morning. 1mg subQ injection.”

Not testedFringe

High-dose Semax, a little Selank in the evening

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: Semax 750–1,500 mcg nasally; a small Selank dose in the evening
How often: Semax daily; Selank in the evening

Route: Nasal spray
Timing: Selank in the evening to smooth the ‘energy crash’

Users said doses below about 1,000 mcg of Semax did nothing for them. This pattern cannot be followed with a 1:1 pre-mixed vial.

Sources (2)

ForumReddit r/Nootropics, /u/julius7009 (Aug 2025) (opens in a new tab)

“My experience is that doses below 1000mcg intranasally don’t do anything.”
ForumReddit r/Nootropics, /u/QuinnMiller123 (Aug 2025) (opens in a new tab)

“the strategy is to use high dose Semax and then use a bit of Selank in the evening to counter the slight energy crash”

Where the numbers come from

Both are Russian medicines sold as nasal drops or sprays (Semax 0.1%, Selank 0.15%). Community doses follow those products’ strengths and the Russian practice of short courses. The pairing (‘Semax for focus, Selank for calm’) has been a nootropic-forum habit since at least 2016; vendors now sell both in one vial.

Home-made sprays and serums

Home-made nasal spray. Community recipes. Not tested.

Original source recordsHide

People do turn Selank 10 mg / Semax 10 mg combo vials into one nasal spray, using the same maths as for a single peptide, applied to each peptide (one user: 5 mL of saline gives 200 mcg of each per spray, 50 sprays). Community only; no data on how stable the two are together.

Best evidence: Community only (Reddit r/Peptides thread on exactly this 10 mg/10 mg combo vial, 2026). Vendors also sell ready-mixed Semax/Selank sprays.

Collected strength records

200 mcg of each per spraySometimes seen

Workings one combo vial is 10 mg Selank + 10 mg Semax (20 mg in all, the figure in peptideMg); 10 mg ÷ 5 mL = 2 mg/mL of each; 5 mL ÷ 50 sprays = 0.1 mL per spray (the poster’s own figures); 2 mg/mL × 0.1 mL = 200 mcg of each per spray.
Sources (3)
- ForumReddit r/Peptides, u/Taladryn (post, 2026-06-16) (opens in a new tab)
  
  “I’ve got a combo vial of Semax 10mg & Selank 10mg and I want to turn it into a nasal spray”
- ForumReddit r/Peptides, u/heartbroken3333 (comment, 2026-06-16) (opens in a new tab)
  
  “5mL sterile saline makes each spray 0.2mg/200mcg. 50 total sprays.”
- ForumReddit r/Peptides, u/heartbroken3333 (comment, 2026-06-16) (opens in a new tab)
  
  “Math doesn’t change if you have another peptide like semax/selank combo.”

Not testedNasal spraySometimes seen

Combo vial dilution table (1, 2 or 4 mL of sterile saline)

Original source-specific report. Collection checked 30 Sep 2026; presentation annotated 2026-10-01. Source date and reporter identity are not separately verified. Exact form and route are limited to the source’s description.

Batch: 1–4 mL

Ingredients and methodHide the recipe

Ingredients

Ingredient	Amount	Role
Selank + Semax combo vial	10 mg + 10 mg	Active
Sterile saline (not bacteriostatic water)	1, 2 or 4 mL	Liquid

Method

Use a metered pump bottle that delivers 0.1 mL per spray.
Dissolve the combo vial in the chosen volume of sterile saline and transfer to the bottle.

Per dose: Per 0.1 mL spray (stated): 1 mL = 1 mg of each; 2 mL = 500 mcg of each; 4 mL = 250 mcg of each.
Preservative: None (saline chosen to avoid irritation).

Not stated: how it’s used.

Sources (4)

ForumReddit r/Peptides, u/Burning-Asteroids (comment, 2026-06-16) (opens in a new tab)

“Find a metered pump nasal spray container, they usually deliver 0.1ml per spray”
ForumReddit r/Peptides, u/Burning-Asteroids (comment, 2026-06-16) (opens in a new tab)

“If you reconstitute the powder with 1 ml of solvent, there will be 1mg of each ingredient in 0.1ml (1spray)”
ForumReddit r/Peptides, u/Burning-Asteroids (comment, 2026-06-16) (opens in a new tab)

“2ml will give you 500mcg of each ingredient 0.1ml, 4ml - 250mcg of each in 1 spray.”
ForumReddit r/Peptides, u/Burning-Asteroids (comment, 2026-06-16) (opens in a new tab)

“I recommend reconstituting with sterile saline, not bacteriostatic water, to prevent irritation of nasal passages.”

Not testedNasal spraySometimes seen

Combo vial in 5 mL sterile saline: 200 mcg of each per spray

Original source-specific report. Collection checked 30 Sep 2026; presentation annotated 2026-10-01. Source date and reporter identity are not separately verified. Exact form and route are limited to the source’s description.

Batch: 5 mL

Ingredients and methodHide the recipe

Ingredients

Ingredient	Amount	Role
Selank + Semax combo vial	10 mg + 10 mg	Active
Sterile saline	5 mL	Liquid

Method

Mix with 5 mL sterile saline (no bacteriostatic water).
Wipe the nozzle with an alcohol wipe before and after each use.

How it’s used: One spray in each nostril; don’t blow your nose for 30 minutes; repeat for a higher dose. A reader worked out 50 sprays lasts 25 days at twice a day.
Per dose: 0.2 mg (200 mcg) per spray (stated); 50 sprays per bottle.
Preservative: None.

The poster said contamination levels are too low to need bacteriostatic water.

Sources (4)

ForumReddit r/Peptides, u/heartbroken3333 (comment, 2026-06-16) (opens in a new tab)

“5mL sterile saline makes each spray 0.2mg/200mcg. 50 total sprays.”
ForumReddit r/Peptides, u/heartbroken3333 (comment, 2026-06-16) (opens in a new tab)

“Always wipe the nasal spray (the part that goes up your nose) with a alcohol wipe to further reduce contamination, before and after.”
ForumReddit r/Peptides, u/heartbroken3333 (comment, 2026-06-16) (opens in a new tab)

“After spraying, don’t blow your nose for 30mins and reapply if you want a higher dose.”
ForumReddit r/Peptides, u/BuddhistL (comment, 2026-08-14) (opens in a new tab)

“if i were to make this and it comes to 50 total sprays that means it will last me 25 days assuming i am doing it twice daily?”

How the method was put together, step by step with its sources

Editorial synthesis and reported strength comparison

This synthesized method combines source records. It has not been validated for preparation, sterility, compatibility, absorption or storage. Select a reported strength to inspect the arithmetic; no strength is selected for you.

Strength per spray 200 mcg of each

Not testedNasal spray

200 mcg of each per spray

Makes 5 mL · about 45 sprays

Synthesized quantities and equipment

1 combo vial (10 mg Selank + 10 mg Semax)
5 mL sterile saline
Liquid, which people disagree on: sterile saline (gentler, but no preservative), bacteriostatic water (preserved, but can burn), or a mix of the two.
An empty metered nasal spray bottle. Many are sold as about 0.1 mL per press, but users report 0.05–0.2 mL, so check yours.
New sterile syringes to measure and move liquid. On a U-100 insulin syringe, 100 units is 1 mL, so 1 unit is 0.01 mL.
Alcohol prep pads, for the vial stopper and the spray tip.

Synthesized steps · not validated

Check your pump first: spray a measured amount of water through it and count the presses. Users report about 0.05–0.2 mL per press.
Wash your hands. Wipe the vial’s rubber stopper with an alcohol pad and let it dry before piercing it.
With a new sterile syringe, add a little of the liquid slowly, aiming it down the inside wall of the vial, not onto the powder.
Swirl or roll the vial gently until the powder dissolves; don’t shake it. Don’t use it if it’s cloudy, discoloured or has particles.
Draw the dissolved peptide back into the syringe and add it gently to the spray bottle.
Rinse the vial with a little more of the liquid and add that to the bottle too, so no peptide is left behind.
Make the bottle up to the full batch volume with the rest of the liquid.
Fit the pump and press it into the air until a fine mist appears; labels say 4–6 presses. Re-prime after 3–7 days unused.
Label the bottle with the date you mixed it and its strength.
Before each dose, blow your nose gently. Close one nostril and aim the tip toward the back of your head, not the middle wall.
Labels differ: some say breathe in gently as you press, others hold your breath. Try not to sneeze or blow your nose straight afterwards.
Afterwards, wipe the tip with an alcohol wipe, put the cap back on and keep the bottle upright.

Each spray: 200 mcg Selank + 200 mcg Semax per 0.1 mL spray
Keeps: Fridge (2–8 °C), upright; don’t freeze. No tested shelf life: pharmacy defaults are 14 days unpreserved, 35 days preserved; saline dilutes bacteriostatic water’s preservative.
How it’s used: One press per nostril at a time: a nostril holds about 0.1 mL, at most 0.15 mL, and more runs out or is swallowed.

Good to know

Bacteriostatic water contains 0.9–1.1% benzyl alcohol as a preservative. Its label covers mixing injections, not nasal use; EU labels warn of allergic reactions.
Some use store-bought saline nasal spray for its preservatives: often benzalkonium chloride, which can irritate the nose with long use, and sometimes benzyl alcohol.
Never tap water: it is not sterile, and CDC researchers warn against it for nasal rinsing.

This method is a guide put together from the sources cited for each item (drug labels, pharmacy and CDC rules, research reviews and community recipes); it is not anyone’s tested protocol. The method’s sources

What people report

User reports
A ready-mixed Semax/Selank spray arrived hot with a strong ‘fishy’/sewage smell; the buyer then reported severe diarrhoea about 2 hours after use. The vendor said the smell was normal. That vendor’s base was phosphate-buffered saline with chitosan, olive oil, eucalyptus oil, glycerin and stevia.
Source: Reddit r/Peptides, u/c0crusader (2026-06-13) (opens in a new tab)
User reports
Mixing two peptides in one bottle: one experienced user advises separate bottles because peptides ‘can integrate and degrade one another over time’; others say Semax and Selank are ‘often sold as a blend’ and one kept a Selank/Adamax mix 2 months with ‘nothing growing’.
Source: Reddit r/Peptides – ‘Combining multiple peptides in one nasal spray container?’ (2023) (opens in a new tab)

Cautions

User reports
Insomnia when Semax is taken more than once a day or late in the day.
Source: Reddit r/Nootropics thread ‘Dosage and dosing schedule of Selank/Semax’ (Nov 2024) (opens in a new tab)
User reports
Anxiety attacks, or feeling far too wired, at higher Semax doses; one user felt no effect at all.
Source: Reddit r/Nootropics thread ‘Semax/Selank effects, side effects, experiences’ (Aug 2025) (opens in a new tab)
User reports
One user who overdid it (about 3 sprays of N-acetyl Semax) described loss of interest, feeling far too wired and odd nerve sensations.
Source: Reddit r/Nootropics thread ‘NA-Semax and NA-Selank dosage guide and questions’ (Jan 2016) (opens in a new tab)

Show all 7 cautionsShow fewer cautions

User reports
Irritability on N-acetyl Semax, including at a 1 mg morning injection; another user had irritability and anxiety from the first 200 mcg Semax dose.
Source: Reddit r/Nootropics thread ‘Permanent aftereffects of Semax/Selank?’ (Apr 2015) (opens in a new tab)
User reports
Headache and nausea when pushing high doses of several nasal peptides together.
Source: Reddit r/Peptides thread ‘Semax, P21, Selank nasal sprays: how much?’ (Jun 2022) (opens in a new tab)
User reports
Users disagree about what liquid to use for a home-made nasal spray. Some warn that bacteriostatic water, which is meant for injection, damages the nasal lining; others argue against saline.
Source: Reddit r/Nootropics thread ‘Selank and Semax: I got injectable, can I use it [nasally]?’ (Nov 2024) (opens in a new tab)
Regulator
The FDA says compounded Semax and Selank may cause immune reactions through aggregation and impurities. It lacks information on whether they would cause harm in people.
Source: FDA: Bulk drug substances that may present significant safety risks (page updated April 22, 2026) (opens in a new tab)

Research notes

No forum protocol for a pre-mixed 10 mg/10 mg vial was found. The forum doses are for separate sprays, where users take about twice as much Semax as Selank; the vendor protocols come from a 5 mg/5 mg blend seller and a clinic. The vendor’s spray arithmetic is internally inconsistent (see notes). Reported effective Semax doses vary more than tenfold (100 mcg to over 1,000 mcg). The N-acetyl amidate forms sold here have little published data of their own. In July 2026 an FDA advisory panel voted to recommend allowing pharmacies to compound Semax (not Selank); the FDA had not decided at the time of writing.

Nasal spray

No source tested Semax and Selank together in one solution. The ‘don’t mix’ advice was given about P21 and P-22-28, not this blend.

70 mg per vial

Beauty Blend

GHK-Cu50 mg
KPV20 mg

Collected rationale, not demonstrated combination efficacy. Ingredient findings do not validate this mixture.

GHK-Cu for collagen and skin repair with KPV for inflammation, aimed at skin appearance.

Community protocols. Reported online. Not clinically validated.

Community reports Collection date: 30 Sep 2026

No protocol was found for a pre-mixed 50 mg/20 mg vial, but users who pair GHK-Cu with KPV, sometimes mixed in one pen, commonly take about 2–3 mg GHK-Cu with 250–600 mcg KPV once a day, for about 8 weeks.

More about this

Their ratio is about 5:1. This vial is 5:2, so the same GHK-Cu dose would bring about twice as much KPV.

Not testedFrequently encountered in reviewed sources

GHK-Cu 2–3 mg + KPV 400–600 mcg once a day, often mixed in one pen

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Forum: Reddit r/Peptides, /u/VIPDX (Jun 2026)
Forum: Reddit r/Peptides, /u/RedditOnTheInnanet (Jun 2026)
Forum: Reddit r/Peptides, /u/1Yetta (Jun 2026)
Forum: Reddit r/Peptides, /u/onrake (Jun 2026)

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: GHK-Cu 2–3 mg with KPV 400–600 mcg (one user: 2 mg GHK-Cu with 250 mcg KPV)
How often: Once a day (nightly); some skip the 5-on-2-off pattern

Route: Injection under the skin
Timing: Nightly (one user)
How long: About 8 weeks per cycle
Cycle: One user advises KPV for no more than 8–12 weeks, while GHK-Cu is run longer
Ramp: One user worked up from about 0.5 mg GHK-Cu
Mixing: Users mixed their own GHK-Cu and KPV in one pen; water volumes were not stated

Calculated from this vendor’s vial ratio (50 mg GHK-Cu : 20 mg KPV): whatever the water volume, each 1 mg of GHK-Cu drawn comes with 400 mcg of KPV. At the users’ 2 mg GHK-Cu, this vial would give 800 mcg KPV, not 400 mcg; at 3 mg it would give 1.2 mg KPV, not 600 mcg.
Users take zinc on the side to offset the copper, and report the usual GHK-Cu sting and welts.

Sources (4)

ForumReddit r/Peptides, /u/VIPDX (Jun 2026) (opens in a new tab)

“I ran 2mg GHK-Cu 400mcg KPV nightly for 8 weeks. I did titrate up from 509mcg GHK-Cu. I put them both in the same pen.”
ForumReddit r/Peptides, /u/RedditOnTheInnanet (Jun 2026) (opens in a new tab)

“3mg/600mcg daily. Reconned together in a pen. Might increase for the second cycle. No reason to do 5/2.”
ForumReddit r/Peptides, /u/1Yetta (Jun 2026) (opens in a new tab)

“I am now doing 3mg GHK-cu and 400mcg KPV.”
ForumReddit r/Peptides, /u/onrake (Jun 2026) (opens in a new tab)

“Standard protocol doses of GHK-cu 2mg and KPV .25mg.”

Not testedSometimes seen

Separate schedules: GHK-Cu 5 days a week, KPV 3 days a week

Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.

Frequency labels describe this collection, not a measured population. Contradictory records remain available.

Details and sourcesHide details

Dose: GHK-Cu 2 mg 5 days a week + KPV 300 mcg 3 days a week
How often: GHK-Cu 5 days a week; KPV 3 days a week

Route: Injection under the skin
How long: About 4 weeks planned

This pattern needs separate vials; a pre-mixed vial cannot give KPV on fewer days than GHK-Cu.

Sources (2)

ForumReddit r/Peptides, /u/28CentSoup (Jun 2026) (opens in a new tab)

“I think I will run KPV 3 days a week 300mcg while running ghkcu 2mg/5 days a week”
ForumReddit r/Peptides, /u/MFpisces23 (Jun 2026) (opens in a new tab)

“You can run GHK-CU indefinitely and KPV for 8-12 weeks max, maybe less, as the science is still emerging.”

Where the numbers come from

User-built pairing: GHK-Cu for skin and hair, KPV for inflammation. The same two peptides sit inside the KLOW blend. The ‘Beauty Blend’ name and its 5:2 ratio did not appear in any forum or clinic source found.

Cautions

Regulator
The FDA says it has found no human exposure data for KPV by any route. It flags possible immune reactions and impurities with injected GHK-Cu.
Source: FDA: Bulk drug substances that may present significant safety risks (page updated April 22, 2026) (opens in a new tab)
User reports
Red welts and stinging at GHK-Cu injection sites (see the GLOW entry for more).
Source: Reddit r/Peptides thread ‘For all my folks running GHK-Cu/KPV separately’ (Jun 2026) (opens in a new tab)
User reports
Itchy injection sites in a user injecting GHK-Cu + KPV near the knee for about 4 weeks.
Source: Reddit r/Peptidesource thread ‘GHK-Cu + KPV for knee issues’ (Feb 2025) (opens in a new tab)

Show all 4 cautionsShow fewer cautions

User reports
The author of the Anela Protocols says she would not go above 2 mg of GHK-Cu and will not recommend injected GHK-Cu for anyone under 25, citing adverse skin reports in younger users.
Source: Krysia’s Substack: ‘Ask Anela’ Q&A with the author of the Anela Protocols (Sep 2026) (opens in a new tab)

Research notes

No source used this vendor’s ‘Beauty Blend’ name or its 5:2 ratio. Users who pair the two use roughly 5:1 (for example 2 mg + 400 mcg, 3 mg + 600 mcg), so this vial delivers about twice their KPV at the same GHK-Cu dose (calculated). Users also disagree on GHK-Cu amounts: some take 3 mg a day, while others (including the Anela protocol author) say not to exceed 2 mg. One Reddit user called GHK-Cu + KPV ‘the Deadpool stack’, a name that means several different things.

Explore the compositions.

KLOW Blend

Community protocols. Reported online. Not clinically validated.

Daily dose keyed to 2 mg GHK-Cu (3 mL mix)

Higher daily dose (about 2.5 mg GHK-Cu)

Loading phase, then maintenance

Same 2 mg target, different water volume

Clinic course (pharmacy-compounded KLOW)

Where the numbers come from

Cautions

Glow Blend

Community protocols. Reported online. Not clinically validated.

Daily skin and repair dose (3 mL mix)

Higher daily dose (about 2.5 mg GHK-Cu)

Smaller shots twice a day

Clinic course (pharmacy-compounded GLOW)

Where the numbers come from

Cautions

BPC-157 / TB4

Community protocols. Reported online. Not clinically validated.

250–500 mcg of each a day, split into one or two shots

Clinic ‘Wolverine Blend’ course

Step-up over 8 weeks (guide-site calculation)

Where the numbers come from

Cautions

BPC-157 / TB4 (double)

Community protocols. Reported online. Not clinically validated.

500 mcg to 1 mg of blend a day (2 mL mix)

High dose for injuries (1 mg of each a day)

Clinic ‘Wolverine Blend’ course

Where the numbers come from

Cautions

Deadpool Blend

Community protocols. Reported online. Not clinically validated.

Where the numbers come from

Cautions

CJC-1295 no DAC / Ipamorelin

Community protocols. Reported online. Not clinically validated.

Bedtime dose, 5 days a week

Two or three shots a day (‘saturation dose’)

Clinic step-up to 1 mg of blend

Where the numbers come from

Cautions

Tesamorelin / Ipamorelin

Community protocols. Reported online. Not clinically validated.

About 1 mg tesamorelin at bedtime

Up to 2 mg tesamorelin at night

Vendor ‘experience level’ tiers

Low-dose prescribed pairing

1 mg tesamorelin + 500 mcg ipamorelin nightly

Where the numbers come from

Cautions

Selank / Semax

Community protocols. Reported online. Not clinically validated.

Nasal spray, 1–2 sprays of each once or twice a day (forum)

Nasal spray (vendor and clinic figures)

Equal amounts of each, 500 mcg to 1 mg a day

Injection under the skin

High-dose Semax, a little Selank in the evening

Where the numbers come from

Home-made nasal spray. Community recipes. Not tested.

Collected strength records

Combo vial dilution table (1, 2 or 4 mL of sterile saline)

Combo vial in 5 mL sterile saline: 200 mcg of each per spray

200 mcg of each per spray

What people report

Cautions

Nasal spray

Beauty Blend

Community protocols. Reported online. Not clinically validated.

GHK-Cu 2–3 mg + KPV 400–600 mcg once a day, often mixed in one pen

Separate schedules: GHK-Cu 5 days a week, KPV 3 days a week

Where the numbers come from

Cautions