N-Acetyl Semax Amidate
Taken for focus and memory
Modified Semax
This entry is the N-acetyl amidated derivative. Parent Semax/Selank studies and approval records do not establish this derivative’s efficacy, safety or approval.
Color key
At a glance
Why people take it. And what backs it up.
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Sharpen focus and memory
People report it, not studied
The main use: a morning nasal spray for sharper focus, memory and mental energy; plain Semax is approved in Russia, but not this form.
Sources (4)
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ForumReddit r/Nootropics: “Experiences trying NA-Semax Amidate” (May 2016) (opens in a new tab)
“I can focus easier on tasks, remember things more easily, my memory in general is more agile.”
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“Been doing intranasal semax in the morning (about 600mcg total split between nostrils) for focus work”
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VendorSimon’s Compounding (compounding pharmacy): N-Acetyl Semax Amidate (opens in a new tab)
“Enhanced Focus & Mental Stamina: Increases dopaminergic signaling, supporting concentration, working memory, and learning speed without causing jitteriness”
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“Semax is a registered drug in Russia and is available as 0.1% and 1% nasal drops.”
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Lift mood and motivation
People report it, not studied
Users take it for low mood and flat motivation; some say it lifts a mental cloud and makes them more driven.
Sources (2)
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“NASA seems to eliminate my internal dialogue, which is helpful for my depressive symptoms.”
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ForumReddit r/Nootropics: same thread (November 2020) (opens in a new tab)
“I take two sprays in the morning and it feels like a cloud is lifted and I’m motivated throughout the day.”
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Focus with ADHD
People report it, not studied
Some with ADHD use it as a stimulant-free focus aid, or alongside Adderall to soften its crash.
Sources (2)
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“I defintely have ADHD , not great focus, mental clarity, and often tired through out the day. I dont want to be on any stimulants”
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“Better yet, for some reason, it completely mitigates the crash and the anxiety of using Adderall!”
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Evidence briefing
Sources checkedWhat the sources support.
Plain Semax is the heptapeptide Met-Glu-His-Phe-Pro-Gly-Pro; FDA briefing material says it is registered in Russia as 0.1% and 1% nasal drops. That status and the limited regional literature do not apply to the catalogue’s N-acetyl Semax amidate: FDA-reviewed Semax is the unmodified peptide (free base or acetate), and the capped amidate form is a different product with no approval established here. In July 2026 the advisory committee recommended plain Semax-related forms for possible 503A bulk listing, but a committee recommendation is not FDA approval or a final listing.
Evidence and any Russian authorization concern the named nasal Semax product; they cannot be transferred to the N-acetyl amidate sold in vials. FDA staff’s briefing found limited route-specific safety/effectiveness evidence.
The research. What’s been studied.
Clinical benefit unverified
TapClick a dotted word to see what it means.
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No verified benefit for this form
The Semax amidate listed here has no verified clinical evidence. Findings reported for plain Semax should not be assigned to this modified form.
What do these labels mean?
- Human evidence: approved-drug data or larger human studies, within the populations and uses studied.
- Early human studies: small, preliminary or limited human studies.
- Animal & lab studies: animal or laboratory findings that do not establish effects in people.
- Unverified: a proposed use or claim without verified supporting clinical evidence.
How each claim was reviewed (4)
Reported effects and evidence.
A citation may support, qualify or correct a claim. Read the study scope; a laboratory result is not an established benefit in people. Each review covers a claim as it was first collected; the tiles above show the collection’s current wording.
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Parent Semax animal study; modified-form mismatch
Review of “Raises BDNF”
Plain Semax increased hippocampal BDNF in rats. This result does not validate N-acetyl amidated Semax.
Limitations and applicability. Entry is N-acetyl amidate. Parent Semax BDNF findings cannot be assigned to that derivative.
Original collected claim and review history
Original title: Raises BDNF
Increases BDNF, a protein that supports neuron growth and learning.
Original label: animal. Preserved for traceability; the reviewed wording above qualifies this record.
Record semax:benefit:1 · Annotated 2026-10-01 · Review method
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Limited human parent-compound research; identity mismatch
Review of “Stroke recovery”
Plain Semax was studied in Russian stroke-rehabilitation cohorts; display this as related-compound evidence, not derivative efficacy.
Limitations and applicability. Foreign status and stroke research concern plain Semax; modified form described on this page has no matching clinical source.
Original collected claim and review history
Original title: Stroke recovery
Used in Russia to support recovery after stroke.
Original label: early. Preserved for traceability; the reviewed wording above qualifies this record.
Record semax:benefit:2 · Annotated 2026-10-01 · Review method
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Unknown — no direct primary source verified. Reported parent-compound clinical claim; direct citation unresolved
Review of “Focus and memory”
Cognitive benefits are reported for plain Semax. Exact primary trial support and relevance to the modified form remain unresolved.
Limitations and applicability. No specific controlled attention/memory trial was verified; modified product is not the same intervention.
Unknown: no direct primary source verified. This does not mean that no research exists.
Original collected claim and review history
Original title: Focus and memory
Russian studies report better attention and memory.
Original label: early. Preserved for traceability; the reviewed wording above qualifies this record.
Record semax:benefit:3 · Annotated 2026-10-01 · Review method
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Parent Semax rodent ischemia research
Review of “Brain protection”
Plain Semax altered neurotrophin-gene expression after experimental rat ischemia; retain the model and molecular-identity limitation.
Limitations and applicability. Molecular gene-expression effects in rodent ischemia are not direct proof of protected human neurons, especially for derivative.
Original collected claim and review history
Original title: Brain protection
Protected neurons in animal models of brain injury.
Original label: animal. Preserved for traceability; the reviewed wording above qualifies this record.
Record semax:benefit:4 · Annotated 2026-10-01 · Review method
The research in detail
Evidence category
Little or no published research
Human and animal studies concern plain Semax and are limited by study size, setting and route. The N-acetyl amidate listed here has no verified clinical evidence. In July 2026, the advisory committee recommended plain Semax forms for possible inclusion on the 503A compounding list; this nonbinding recommendation is not FDA drug approval or a final list decision.
Evidence is assessed claim by claim above. Regulatory approval, study design, findings and relevance are separate questions; there is no single evidence score.
Risks and unknowns. What to know first.
Collected cautions follow. These are not an exhaustive or independently validated safety assessment; claim references do not automatically support every caution.
- Generally reported as well tolerated in Russian studies; nasal irritation is common.
- Independent long-term data are lacking.
What people report
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User reports
Irritability or a short temper, especially when combined with coffee.
Source: Peptide Critic forum, ‘Semax and Caffeine’ (opens in a new tab)
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User reports
Anxiety, a racing heart and badly disturbed sleep lasting days after Semax or Selank amidate sprays (forms not always stated); one user said sleep took about 10 days to recover after 4 sprays.
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User reports
Headaches (especially above the usual dose), feeling anxious or sick, feeling spaced out, and fading effects over time are described in user anecdotes; a few biohackers link it to hair loss.
Source: Jay Campbell, ‘Semax vs Selank Nasal Peptides’ (opens in a new tab)
Show all 10 cautionsShow fewer cautions
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User reports
Nosebleeds and stinging from homemade sprays made with bacteriostatic water (which contains benzyl alcohol); users switched to saline.
Source: Peptide Critic forum, ‘Semax Spray nose bleed’ (opens in a new tab)
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User reports
Many feel little or nothing, and several say the amidate form felt weaker than plain Semax.
Source: Reddit r/Peptides, ‘Semax/Selank vs Adamax/Adalenk’ thread (opens in a new tab)
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Case report
One FDA adverse-event report (not a published case): a consumer had eye pain and burning, was hospitalized, and still had pain a year later after using plain Semax 0.1% nasal drops bought online from CosmicNootropic.
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Study
FDA found human safety data only for nasal use (small studies), none for injection under the skin, and noted one study suggesting anti-clotting effects that raise a possible bleeding concern.
Source: FDA briefing document on semax, July 2026 (opens in a new tab)
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Regulator
The FDA lists semax (heptapeptide) among bulk drug substances that may present significant safety risks in compounding, citing possible immune reactions and limited safety information.
Source: FDA, Certain bulk drug substances that may present significant safety risks (opens in a new tab)
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Regulator
In July 2026 the FDA’s compounding advisory committee narrowly voted to recommend semax for the list of substances pharmacies may compound, against FDA staff advice. The vote is advisory and concerns plain semax, not the amidate.
Source: AJMC, ‘FDA Panel Backs 6 Peptides for Compounding’ (opens in a new tab)
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Regulator
The pharmacy Tailor Made Compounding, whose clinic protocol is listed above, pleaded guilty to distributing unapproved new drugs including Semax (2018–2020).
Source: US Department of Justice press release, February 2021 (opens in a new tab)
Published dosing records. What the label abroad says.
Historical label and trial records for the named product, route, population and indication. These records are not a personal schedule or a validated regimen for a catalog vial. Source attribution does not establish applicability.
Semax 0.1%
Memory and thinking problems caused by disease of the brain’s blood vessels, including after mini-strokes (Russia)
- Each dose, for 10–14 days
- 200–2,000 mcg
- How
- Drops in the nose
- How often
- 4 times a day
- How long
- 10–14 days, repeated if needed
Limit. Up to 8,000 mcg a day (the label’s daily dose is 800–8,000 mcg, or 7–70 mcg/kg). No more than 2–3 drops in each nostril at a time; larger doses are split into rounds 10–15 minutes apart.
- This label covers plain Semax nose drops, not the N-Acetyl Semax Amidate the vendor sells. That chemically modified form is a different molecule: this label does not cover it, and no published study of it in people was found.
- One drop of the 0.1% solution holds 50 mcg. The label gives 2–3 drops in each nostril, 4 times a day.
- After a head injury, brain surgery or anesthesia: 1,400–3,500 mcg (40–50 mcg/kg) 3 times a day for 3–5 days, extendable to 14 days. Not for under-18s after brain surgery.
- Children 7 and older with minimal brain dysfunction (including ADHD): 1–2 drops in each nostril (5–6 mcg/kg) twice a day, 200–400 mcg a day, for 30 days.
- Optic nerve disease (adults only): 2–3 drops in each nostril 2–3 times a day (600–900 mcg a day) for 7–10 days.
Semax 1%
The first (acute) period of a moderate or severe ischemic stroke, alongside other treatment (Russia; adults)
- Days 1–10, each dose (moderate stroke)
- 2,000–3,000 mcg
- How
- Drops in the nose
- How often
- 3–4 times a day, 3–4 hours apart (moderate stroke)
- How long
- 10 days
Limit. Up to 12,000 mcg a day for a moderate stroke and up to 20,000 mcg a day for a severe stroke (the label’s daily doses are 6,000–12,000 mcg and 12,000–20,000 mcg).
- This label covers plain Semax nose drops, not the N-Acetyl Semax Amidate the vendor sells. That chemically modified form is a different molecule: this label does not cover it, and no published study of it in people was found.
- One drop of the 1% solution holds 500 mcg. For a moderate stroke, each dose is 2–3 drops in each nostril.
- Severe stroke: 3–4 drops in each nostril (3,000–4,000 mcg) per dose, 4–5 times a day, 2.5–3 hours apart.
- Not for people under 18.
This schedule comes from the cited national product label. Authorization and availability can change: check the current local status and exact formulation. A research vial is not the labeled product.
Community protocols. Reported online. Not clinically validated.
Community reports Collection date: 30 Sep 2026NA-Semax Amidate users most often take about 200–600 mcg a day by nasal spray (1–3 sprays of a 100–300 mcg spray) in the morning, or inject about 300 mcg–1 mg under the skin from a research vial.
More about this
Practice varies widely (early users injected 1–2 mg twice a day), and many posts don’t say whether they mean plain Semax or the amidate form.
Ready-made NA-Semax Amidate nasal spray
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
- Forum: Longecity forum, ‘Semax + Selank + PhenylPiracetam Hydrazide… Ultimate Stack’ (2016)
- Practitioner: Jay Campbell, ‘Semax vs Selank Nasal Peptides’
- Forum: Reddit r/Peptides, ‘N-Acetyl Semax Amidate’ nasal spray thread (2026)
- Vendor: Ceretropic, N-Acetyl Semax Amidate Spray product page (2015, archived)
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- 100–300 mcg per spray; usually 200–600 mcg a day, up to 900 mcg
- How often
- Once a day, sometimes a second dose in the early afternoon
- Route
- Nasal spray
- Timing
- Morning; users avoid late-day doses because it is stimulating
- How long
- Often 2–4 weeks; some use it most days for months
- Cycle
- Patterns seen: 10–20 days then a break; 2 weeks on; 3 weeks on and 1 week off; or weekdays only
- Ramp
- Jay Campbell’s version: 600 mcg a day (2 pumps of a 0.3% spray), rising to 900 mcg (3 pumps) if needed; up to 30 days at the higher dose or 60 days at 600 mcg, then an equal rest
- Mixing
- Ready-made bottles: 10 mg in 10 mL (100 mcg per spray) or 30 mg in 10 mL (300 mcg per spray); other brands sell 200–240 mcg per spray
- The 100 or 300 mcg-per-spray bottle was sold by the vendor Ceretropic by 2015, and current vendors (for example Nootropic Source) still use its product wording; American Peptides sells 100 mcg per spray.
- Jay Campbell’s 0.3% spray protocol does not say which form of Semax it means; 0.3% matches the 300 mcg-per-spray amidate bottles.
- Several users feel little from the amidate form, or less than from plain Semax.
- Vendors also sell a ready-made spray blending NA-Semax Amidate and NA-Selank Amidate (30 mg of each).
Sources (4)
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“600 mcg N-Acetyl Semax Amidate (nasal spray)”
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PractitionerJay Campbell, ‘Semax vs Selank Nasal Peptides’ (opens in a new tab)
“Initial Dose: 600 mcg daily (2 pumps).”
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ForumReddit r/Peptides, ‘N-Acetyl Semax Amidate’ nasal spray thread (2026) (opens in a new tab)
“Usually 200mcg in the morning, maybe again early after noon works well.”
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VendorCeretropic, N-Acetyl Semax Amidate Spray product page (2015, archived) (opens in a new tab)
“Includes one bottle of either 100mcg or 300mcg solution. Each bottle contains 10ml total volume and either 10mg or 30mg total N-Acetyl Semax Amidate.”
Injection under the skin from a research vial
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
- Practitioner: Jay Campbell, ‘Semax vs Selank Nasal Peptides’
- Forum: Peptide Critic forum, ‘Adamax…. Get the real one…’
- Forum: Reddit r/Peptides, ‘correct dosage for Sub-Q N-Acetyl Semax Amidate?’ thread
- Vendor: Smarter Nootropics (information site linked from Ceretropic’s product pages), Semax page (2019, archived)
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- 300 mcg–1 mg (about 400–600 mcg is typical for the amidate)
- How often
- Once a day, or every other day
- Route
- Injection under the skin
- Timing
- Morning
- How long
- About 4–8 weeks
- Cycle
- One posted plan: 1 month on, 2 weeks off, a couple of times a year; others skip weekends
- Ramp
- Forum advice for the amidate: start around 400 mcg and go to 600 mcg
- Mixing
- 5 mg vial + 2 mL bacteriostatic water; 10–30 mg vials with 2–3 mL
- Many research vials are sold simply as ‘Semax’ (usually the plain form); injected doses for plain Semax are similar (500 mcg–1 mg).
- Some users say injection feels weaker or slower than nasal use; others prefer it because it lasts all day.
- FDA found no studies of Semax given by injection under the skin.
Sources (4)
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PractitionerJay Campbell, ‘Semax vs Selank Nasal Peptides’ (opens in a new tab)
“For biohackers aiming to boost thinking skills, a common plan is to inject 500 mcg to 1 mg of Semax subcutaneously each day, depending on how the person reacts.”
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ForumPeptide Critic forum, ‘Adamax…. Get the real one…’ (opens in a new tab)
“For NASA I’d start higher at 400 and go to 600 the first time.”
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ForumReddit r/Peptides, ‘correct dosage for Sub-Q N-Acetyl Semax Amidate?’ thread (opens in a new tab)
“I heard people take anywhere from 300-600mcg some people claiming even 1.2mg.”
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“For nootropic purposes, a dose 0.25-1mg per day would seem suitable.”
Show all 6 protocolsShow fewer protocols
Homemade nasal spray from a 10 mg research vial
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
- Forum: Peptide Critic forum, ‘Semax Nasal Spray’
- Forum: Peptide Critic forum, ‘Semax vs Selank vs Pinealon’
- Forum: Peptide Critic forum, ‘Semax/selank daily or as needed’
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- About 100–300 mcg per spray, 1–2 sprays
- How often
- Once a day, with extra sprays on demanding days
- Route
- Nasal spray
- Timing
- Morning
- Ramp
- Start with one spray, then two
- Mixing
- 10 mg vial dissolved in 1–2 mL bacteriostatic water, then 2–5 mL saline added in the spray bottle; many use saline only to avoid stinging and nosebleeds
- Most of these users buy vials labelled ‘Semax’ (usually the plain form); the same method is used for NA-Semax Amidate vials.
- Users say one nasal dose lasts about 6–8 hours.
Sources (3)
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ForumPeptide Critic forum, ‘Semax Nasal Spray’ (opens in a new tab)
“I used 2 mL Bac water and 5 mL saline to make a nasal spray with 10mg of Semax.”
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ForumPeptide Critic forum, ‘Semax vs Selank vs Pinealon’ (opens in a new tab)
“Ea Semax and Selank are 10mg. I recon’ 1ml of BAC. Add into the sprayer and then add 2ML of saline. I am guesstamating 100-300mcgs.”
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ForumPeptide Critic forum, ‘Semax/selank daily or as needed’ (opens in a new tab)
“Subq 1mg and nasal 100-250mcgs seems to last 6-8hours.”
Plain Semax 0.1% drops (Russian pharmacy product), copying the label
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
- Vendor: CosmicNootropic, Semax nasal drops product page
- Forum: Longecity forum, ‘Semax experiences?’ page 2 (2015)
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- 2 drops in each nostril (50 mcg per drop, about 200 mcg per dose)
- How often
- 1–2 times a day
- Route
- Nasal drops
- How long
- 3–14 days
- This is the plain form, not NA-Semax Amidate; sellers and users copy the Russian label, which is compiled separately.
Sources (2)
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VendorCosmicNootropic, Semax nasal drops product page (opens in a new tab)
“The standard Semax dosage is 2 drops in each nostril, administered 1–2 times per day depending on the regimen.”
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ForumLongecity forum, ‘Semax experiences?’ page 2 (2015) (opens in a new tab)
“My initial dose was two drops per nostril, for a total of 200mcg.”
Compounded clinic spray or injection (plain Semax)
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
- Vendor: Tailor Made Compounding peptide catalog (compounding pharmacy), Semax page
- Clinic: Transform You clinic, Semax page
- Practitioner: Ben Greenfield, peptide resources page (recommended stacks, cognitive health)
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- Nasal: two sprays of a 7,500 mcg per mL spray (spray size not stated). Injection: 100 mcg a day (Tailor Made Compounding chart). Other clinics sell 1.25 mg per mL sprays and 2.5 mg per mL injections
- How often
- Once a day
- Route
- Nasal spray, or injection under the skin into the belly
- Mixing
- Pharmacy-made: 3 mL nasal spray at 7,500 mcg per mL, or 5 mL vial at 1,000 mcg per mL
- These US clinic products were plain Semax, not the amidate; Tailor Made Compounding supplied clinics from 2018 to 2020.
- At a typical 0.1 mL per spray, two sprays of the pharmacy spray would be about 1,500 mcg.
- Clinics often pair it with Selank.
- Ben Greenfield’s list gives 0.5–1.0 mg a day without naming the form or route.
Sources (3)
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VendorTailor Made Compounding peptide catalog (compounding pharmacy), Semax page (opens in a new tab)
“Suggested dosage: Spray two sprays intranasally once daily.”
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ClinicTransform You clinic, Semax page (opens in a new tab)
“It comes in the form of a nasal spray which is administered daily. It is most often combined with another peptide called Selank for additive effects.”
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PractitionerBen Greenfield, peptide resources page (recommended stacks, cognitive health) (opens in a new tab)
“Semax: 0.5-1.0 mg per day”
High-dose injection (early adopters, 2015)
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
- Forum: Longecity forum, ‘Semax experiences?’ page 2 (2015)
- Forum: Longecity forum, ‘Semax experiences?’ page 2 (2015)
- Forum: Reddit r/Peptides, ‘Semax users. Amidate?’ (2024)
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- 1–2 mg per dose
- How often
- Once or twice a day
- Route
- Injection under the skin
- Timing
- Morning, and early afternoon for the second dose
- How long
- Weeks; one user planned at least 2 months
- Cycle
- Weekdays on, weekends off
- Ramp
- 1 mg in the morning, then an extra 0.5–1 mg in the afternoon, later 2 mg twice a day
- Mixing
- 30 mg of powder
- The 2015 posters were early buyers of Ceretropic’s N-Acetyl Semax Amidate powder; a 2024 Reddit user takes 1.5 mg twice a day of a vial sold simply as ‘Semax’ (plain form).
Sources (3)
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ForumLongecity forum, ‘Semax experiences?’ page 2 (2015) (opens in a new tab)
“Started out with 1mg in the morning for a few days then did 1mg in the morning and about .5mg in the after noon”
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ForumLongecity forum, ‘Semax experiences?’ page 2 (2015) (opens in a new tab)
“Started my first dose of the week at 2mg in the morning and just took another 2mg shortly after lunch.”
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ForumReddit r/Peptides, ‘Semax users. Amidate?’ (2024) (opens in a new tab)
“I’ve been using something called “Semax” 1.5mg twice a day and love the effect.”
Where the numbers come from
Semax is a Russian drug (plain heptapeptide, 0.1% and 1% nasal drops). The N-acetyl amidate form was made and sold by the nootropics vendor Ceretropic by early 2015, as nasal sprays (100 or 300 mcg per spray) and powder; its product page says ‘we’ acetylated and amidated Semax. It has never been studied in people. Doses were borrowed from the plain drug’s label and from Smarter Nootropics, an information site linked from Ceretropic’s pages (0.25–1 mg a day by nose or injection), and later vendors reused Ceretropic’s product wording. Clinic protocols for compounded plain Semax came from the Tailor Made Compounding pharmacy (2018–2020). Jay Campbell’s pages summarize both the spray and the 500 mcg–1 mg injection plans.
Home-made sprays and serums
Home-made nasal spray. Community recipes. Not tested.
Original source recordsHide
Home-made Semax nasal sprays are among the most common DIY peptide sprays. People dissolve a 5–30 mg vial in saline, bacteriostatic water or a mix of the two and put it in a bottle that sprays about 0.1 mL; reported strengths run from about 100 to 333 mcg per spray. All of this is community practice, not tested.
Best evidence: Approved product: Russian Semax 0.1% nose drops (1 mg per mL, 50 mcg per drop, methylparaben preservative) – the reference point people copy (covered elsewhere). Home-made sprays: community only (Reddit r/Nootropics since 2016, r/Peptides), plus one US compounding pharmacy strength (Tailor Made Compounding, 7,500 mcg per mL, 3 mL, 2020 listing).
Collected strength records
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100 mcg per sprayFrequently encountered in reviewed sources
Workings The 2016 post states 30 mg in 30 mL = 1,000 mcg per mL = 100 mcg per 0.1 mL. Calculated for the other posts: 12 mg ÷ 12 mL and 5 mg ÷ 5 mL are also 1 mg/mL, so 100 mcg per 0.1 mL spray.
Sources (6)
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ForumReddit r/Nootropics, u/gramphibian (post, 2016-04-06) (opens in a new tab)
“Therefore, 30mg Semax diluted in 30ml water would be 30,000mcg/30 = 1000mcg per ml OR 100mcg per .1ml (the amount dispensed by the sprayer).”
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ForumReddit r/Nootropics, u/gramphibian (post, 2016-04-06) (opens in a new tab)
“First, you’ll want to get a 30mg vial of Semax from Ceretropic, along with a 30ml bottle of bacteriostatic water.”
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ForumReddit r/Nootropics, u/gramphibian (post, 2016-04-06) (opens in a new tab)
“Since your calibrated sprayer dispenses .1ml per spray, you can know with relative certainty that you’re getting 100mcg every time you dispense the sprayer.”
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ForumReddit r/Peptides, u/Ill-Bet-6094 (post, 2025-03-22) (opens in a new tab)
“I’m combining 30ml saline and 30mg lyophilized Semax. So recon is 1mg/ml.”
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ForumReddit r/Peptides, u/twistedspin (comment, 2025-04-10) (opens in a new tab)
“My vials have been 12mg, I use 12ml total (like 11.5 of that is the saline). My sprayers have been right about .1ml per spray”
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ForumReddit r/Peptides, u/beaubafett78 (comment, 2026-02-11) (opens in a new tab)
“That gives you an easy 5mg/5ml or 10mg/5ml ratio to work with.”
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200 mcg per spraySometimes seen
Workings 10 mg ÷ 5 mL = 2 mg/mL; × 0.1 mL = 200 mcg per spray. No spray size given, so 0.1 mL is assumed (approved sprays deliver about 0.1 mL per press); with the poster’s own bottle, just under 4 sprays made 1,000 mcg (about 250–260 mcg per spray).
Sources (2)
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ForumReddit r/Peptides, u/beaubafett78 (comment, 2026-02-11) (opens in a new tab)
“That gives you an easy 5mg/5ml or 10mg/5ml ratio to work with.”
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ForumReddit r/Peptides, u/beaubafett78 (comment, 2026-02-11) (opens in a new tab)
“Pop the top and pull 1ml into your syringe and reconstitute, dump the rest (4ml) of the ampule into the spray bottle”
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250 mcg per spraySometimes seen
Workings 2 mL + 2 mL = 4 mL; 10 mg ÷ 4 mL = 2.5 mg/mL, as the poster states; × 0.1 mL = 250 mcg per spray.
Sources (5)
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ForumReddit r/Peptides, u/SerChuckForce (comment, 2026-09-27) (opens in a new tab)
“Because I purchased 10 mg of semax, it will last longer than I was comfortable only having saline as the reconstitution liquid.”
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ForumReddit r/Peptides, u/SerChuckForce (comment, 2026-09-27) (opens in a new tab)
“I purchased a Snoot spray bottle on Amazon which has a .1 ml per spray dose.”
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ForumReddit r/Peptides, u/SerChuckForce (comment, 2026-09-27) (opens in a new tab)
“I mixed 2 ml of BAC water in the vial and then withdrew the reconstituted semax into a syringe.”
-
ForumReddit r/Peptides, u/SerChuckForce (comment, 2026-09-27) (opens in a new tab)
“I then added 2 ml of saline to my sterile nasal spray bottle and added the reconstituted semax for a final concentration of 2.5 mg/ml.”
-
ForumReddit r/Peptides, u/SerChuckForce (comment, 2026-09-27) (opens in a new tab)
“added the reconstituted semax for a final concentration of 2.5 mg/ml. This results in 250 mcg per spray.”
-
-
333 mcg per spraySometimes seen
Sources (3)
-
ForumReddit r/Peptides, u/Zealousideal-Lab4945 (comment, 2025-08-24) (opens in a new tab)
“I used 1ml of sterile water to recon, then pulled it out and into a new 10ml Snoot bottle, and then you can pour in saline”
-
ForumReddit r/Peptides, u/Zealousideal-Lab4945 (comment, 2025-08-24) (opens in a new tab)
“I used Amazon basic nasal spray because it has some preservatives in it. I used 2ml for a concentration of 10mg/3ml.”
-
ForumReddit r/Peptides, u/Zealousideal-Lab4945 (comment, 2025-08-24) (opens in a new tab)
“If the bottle sprays .1ml per spray then each spray is 333mcg.”
-
30 mg in 30 mL: 100 mcg per spray
Original source-specific report. Collection checked 30 Sep 2026; presentation annotated 2026-10-01. Source date and reporter identity are not separately verified. Exact form and route are limited to the source’s description.
- Forum: Reddit r/Nootropics, u/gramphibian (post, 2016-04-06)
- Forum: Reddit r/Nootropics, u/gramphibian (post, 2016-04-06)
- Forum: Reddit r/Nootropics, u/gramphibian (post, 2016-04-06)
- Forum: Reddit r/Peptides, u/Ill-Bet-6094 (post, 2025-03-22)
- Forum: Reddit r/Peptides, u/Ill-Bet-6094 (comment, 2025-03-25)
- Forum: Reddit r/Peptides, u/Majalisk (comment, 2026-09-25)
- Forum: Reddit r/Peptides, u/Distinct_Hornet_4629 (comment, 2025-11-12)
- Forum: Reddit r/Nootropics, u/Sad_Mountain5336 (comment, 2026-04-07)
- Forum: Reddit r/Peptides, u/hydez (comment, 2025-03-20)
- Forum: Reddit r/Nootropics, u/MisterYouAreSoDumb (comment, 2016-01-07)
- Forum: Reddit r/Peptides, u/Feign_Interest (comment, 2025-11-12)
Batch: 30 mL
Ingredients and methodHide the recipe
Ingredients
| Ingredient | Amount | Role |
|---|---|---|
| Semax (peptide powder) | 30 mg | Active |
| Bacteriostatic water (2016 post) or saline (2025 post) | 30 mL | Liquid |
Method
- Inject a little of the liquid into the Semax vial and let the powder dissolve.
- Move the solution back into the 30 mL bottle of liquid; rinse the Semax vial twice with the mixture to get all of it.
- Fill a ‘calibrated’ nasal sprayer that delivers 0.1 mL per press.
- Keep the bottle in the fridge.
- How it’s used
- Not stated in the 2016 recipe. The 2025 poster started at 1 spray per nostril, then decided that was ‘pretty much microdosing’ and increased.
- Per dose
- 100 mcg per 0.1 mL spray (stated by both posters).
- Preservative
- Bacteriostatic water (benzyl alcohol) in the 2016 version; none stated in the saline version.
- Storage
- Fridge after mixing.
- The 2016 post scales the same method: 60 mg in 30 mL = 200 mcg per spray; 120 mg in 30 mL = 400 mcg per spray.
- 30 mg in 30 mL is 1 mg per mL, the same strength as the Russian Semax 0.1% drops.
- Bottles: the 2016 post links an Amazon ‘calibrated nasal sprayer’; the 2025 poster used a Snoot! bottle said to spray 0.1 mL.
- Bottle output varies: users report about 0.1–0.13 mL, 0.133 mL and 0.15–0.20 mL per press. Their advice is to spray 10 mL of water through the empty bottle (three times) and count the presses.
- Storage claims vary widely: a vendor (2016) said Semax spray stayed effective after a year at room temperature; another user said N-acetyl amidate powders already contain preservatives. Neither claim is backed by data.
Not stated: how long it keeps.
Sources (11)
-
ForumReddit r/Nootropics, u/gramphibian (post, 2016-04-06) (opens in a new tab)
“First, you’ll want to get a 30mg vial of Semax from Ceretropic, along with a 30ml bottle of bacteriostatic water.”
-
ForumReddit r/Nootropics, u/gramphibian (post, 2016-04-06) (opens in a new tab)
“Since your calibrated sprayer dispenses .1ml per spray, you can know with relative certainty that you’re getting 100mcg every time you dispense the sprayer.”
-
ForumReddit r/Nootropics, u/gramphibian (post, 2016-04-06) (opens in a new tab)
“once you reconstitute you’ll want to store your spray bottle in the fridge to preserve potency.”
-
ForumReddit r/Peptides, u/Ill-Bet-6094 (post, 2025-03-22) (opens in a new tab)
“I’m combining 30ml saline and 30mg lyophilized Semax. So recon is 1mg/ml.”
-
ForumReddit r/Peptides, u/Ill-Bet-6094 (comment, 2025-03-25) (opens in a new tab)
“Found out above that I was pretty much microdosing at 1 spray per nostril, so I started increasing today.”
-
ForumReddit r/Peptides, u/Majalisk (comment, 2026-09-25) (opens in a new tab)
“They seem to generally dispense .1-.13ml per depression”
-
ForumReddit r/Peptides, u/Distinct_Hornet_4629 (comment, 2025-11-12) (opens in a new tab)
“Mine ended up being 0.133ml per spray”
-
ForumReddit r/Nootropics, u/Sad_Mountain5336 (comment, 2026-04-07) (opens in a new tab)
“The spray bottle shoots around 0.15ml - 0.20ml.”
-
ForumReddit r/Peptides, u/hydez (comment, 2025-03-20) (opens in a new tab)
“Fill bottle with 10ml water. Count how many times you have to spray until empty. Do that 3 times.”
-
ForumReddit r/Nootropics, u/MisterYouAreSoDumb (comment, 2016-01-07) (opens in a new tab)
“We’ve tested Semax left out at room temperature for a year, and it was still effective.”
-
ForumReddit r/Peptides, u/Feign_Interest (comment, 2025-11-12) (opens in a new tab)
“If you’re using NA-Selank Amidate or NA-Semax Amidate, there’s already preservatives in the lyophilized peps”
30 mg in 10 mL: 300 mcg per spray
Original source-specific report. Collection checked 30 Sep 2026; presentation annotated 2026-10-01. Source date and reporter identity are not separately verified. Exact form and route are limited to the source’s description.
- Practitioner: Jay Campbell – ‘Semax peptide: benefits, dosage and side effects’ (blog, updated 19 Mar 2021)
- Practitioner: Jay Campbell blog, quoting Dr. William Seeds, Peptide Protocols Vol. 1
- Forum: Reddit r/Peptides, u/nosigoat (comment, 2023-10-26)
- Forum: Reddit r/Peptides, u/mephistopheles2u (comment, 2023-10-26)
Batch: 10 mL
Ingredients and methodHide the recipe
Ingredients
| Ingredient | Amount | Role |
|---|---|---|
| Semax or N-acetyl Semax amidate | 30 mg | Active |
| Liquid (type not stated in the blog) | 10 mL | Liquid |
- How it’s used
- 1–2 sprays in each nostril (blog). Doses quoted in the same blog: 750–1,000 mcg once a day by nose (Dr. William Seeds, Peptide Protocols); 0.5–1 mg a day (Ben Greenfield, Boundless).
- Per dose
- 300 mcg per spray, for sprays of about 0.1–0.13 mL (stated).
- A 2023 r/Peptides poster planned the same ‘30mg/10mL mix’; a reply noted bottles range from 0.1 to 0.15 mL per spray.
Sources (4)
-
“If your bottle contains 30 mg of Semax in a 10 mL solution, and each spray contains around 0.1-0.13 mL of solution, you’re looking at 300 mcg”
-
PractitionerJay Campbell blog, quoting Dr. William Seeds, Peptide Protocols Vol. 1 (opens in a new tab)
“750-1000 mcg once a day if taken intranasally, 100-300 mcg once a day if injected subcutaneously”
-
ForumReddit r/Peptides, u/nosigoat (comment, 2023-10-26) (opens in a new tab)
“I believe in that study they did a 30mg/10mL mix, which is what I’m thinking of doing as well”
-
ForumReddit r/Peptides, u/mephistopheles2u (comment, 2023-10-26) (opens in a new tab)
“what is the volume of each spray in your particular bottle (they range from 0.1ml to 0.15 ml typically.”
12 mg in 12 mL, topped up with store-bought saline spray
Original source-specific report. Collection checked 30 Sep 2026; presentation annotated 2026-10-01. Source date and reporter identity are not separately verified. Exact form and route are limited to the source’s description.
- Forum: Reddit r/Peptides, u/twistedspin (comment, 2025-04-10)
- Forum: Reddit r/Peptides, u/twistedspin (comment, 2025-04-10)
- Forum: Reddit r/Nootropics, u/vectorizer99 (comment, 2026-04-07)
Batch: 12 mL
Ingredients and methodHide the recipe
Ingredients
| Ingredient | Amount | Role |
|---|---|---|
| Semax | 12 mg | Active |
| Distilled water (to dissolve in the vial) | a little (amount not stated) | Liquid |
| Commercial saline nasal spray (with preservatives) | most of the 12 mL total (‘like 11.5 of that is the saline’) | Liquid / preservative |
Method
- Dissolve the powder in the vial with a little distilled water.
- Add it to the spray bottle and fill to 12 mL total with commercial saline nasal spray.
- Per dose
- Calculated: 12 mg ÷ 12 mL = 1 mg per mL × 0.1 mL per spray = 100 mcg per spray (spray volume from the same poster).
- Preservative
- Relies on the preservatives in the store-bought saline spray.
- Keeps for
- The poster said the preservatives keep it ‘OK to use for much longer’.
- Many store-bought 0.65% saline sprays contain benzalkonium chloride and benzyl alcohol (‘phenylcarbinol’); see the general facts.
- Others recommend the same approach (‘Reconstitute with commercial nasal spray containing preservatives’).
Not stated: how it’s used and storage.
Sources (3)
-
ForumReddit r/Peptides, u/twistedspin (comment, 2025-04-10) (opens in a new tab)
“for the main liquid it’s good to use commercial saline nasal spray as it has preservatives etc that will keep it OK to use for much longer.”
-
ForumReddit r/Peptides, u/twistedspin (comment, 2025-04-10) (opens in a new tab)
“My vials have been 12mg, I use 12ml total (like 11.5 of that is the saline). My sprayers have been right about .1ml per spray”
-
ForumReddit r/Nootropics, u/vectorizer99 (comment, 2026-04-07) (opens in a new tab)
“Reconstitute with commercial nasal spray containing preservatives. Simple easy cheap.”
10 mg in 10 mL bacteriostatic water: 100 mcg per 0.1 mL (planned)
Original source-specific report. Collection checked 30 Sep 2026; presentation annotated 2026-10-01. Source date and reporter identity are not separately verified. Exact form and route are limited to the source’s description.
- Forum: Reddit r/Peptides, u/Maastrooooo (post, 2025-08-24)
- Forum: Reddit r/Peptides, u/Really_Oh_My (comment, 2025-08-24)
Batch: 10 mL
Ingredients and methodHide the recipe
Ingredients
| Ingredient | Amount | Role |
|---|---|---|
| Semax | 10 mg | Active |
| Bacteriostatic water | 10 mL | Liquid / preservative |
- How it’s used
- Planned 300 mcg a day, about 33 servings.
- Per dose
- 100 mcg per 0.1 mL (stated plan).
- Preservative
- Bacteriostatic water.
- Replies told the poster not to spray bacteriostatic water in the nose and to use saline or deionised water; another said bacteriostatic water ‘will preserve it. Mine is going on 7 weeks now’.
Sources (2)
-
ForumReddit r/Peptides, u/Maastrooooo (post, 2025-08-24) (opens in a new tab)
“if i reconstituted it with 10ml of bac water I could get 100mcg per 0.1 ml I think, with a 300mcg dose daily this would give me around 33 servings.”
-
ForumReddit r/Peptides, u/Really_Oh_My (comment, 2025-08-24) (opens in a new tab)
“Mixing with BAC will preserve it. Mine is going on 7 weeks now. But, please don’t spray BAC up your nose.”
10 mg in 4 mL (2 mL bacteriostatic water + 2 mL saline): 250 mcg per spray
Original source-specific report. Collection checked 30 Sep 2026; presentation annotated 2026-10-01. Source date and reporter identity are not separately verified. Exact form and route are limited to the source’s description.
- Forum: Reddit r/Peptides, u/SerChuckForce (comment, 2026-09-27)
- Forum: Reddit r/Peptides, u/SerChuckForce (comment, 2026-09-27)
- Forum: Reddit r/Peptides, u/SerChuckForce (comment, 2026-09-27)
Batch: 4 mL
Ingredients and methodHide the recipe
Ingredients
| Ingredient | Amount | Role |
|---|---|---|
| Semax | 10 mg | Active |
| Bacteriostatic water | 2 mL | Liquid / preservative |
| Saline | 2 mL | Liquid |
Method
- Add 2 mL bacteriostatic water to the Semax vial and draw it into a syringe.
- Put 2 mL saline in a sterile spray bottle and add the dissolved Semax.
- Per dose
- 250 mcg per spray (stated; bottle sprays 0.1 mL).
- Preservative
- Bacteriostatic water (about half the liquid).
- The poster used bacteriostatic water because a 10 mg vial lasts longer than they were comfortable with using saline alone.
Not stated: how it’s used.
Sources (3)
-
ForumReddit r/Peptides, u/SerChuckForce (comment, 2026-09-27) (opens in a new tab)
“I purchased a Snoot spray bottle on Amazon which has a .1 ml per spray dose.”
-
ForumReddit r/Peptides, u/SerChuckForce (comment, 2026-09-27) (opens in a new tab)
“I mixed 2 ml of BAC water in the vial and then withdrew the reconstituted semax into a syringe.”
-
ForumReddit r/Peptides, u/SerChuckForce (comment, 2026-09-27) (opens in a new tab)
“added the reconstituted semax for a final concentration of 2.5 mg/ml. This results in 250 mcg per spray.”
10 mg in a 5 mL saline ampule
Original source-specific report. Collection checked 30 Sep 2026; presentation annotated 2026-10-01. Source date and reporter identity are not separately verified. Exact form and route are limited to the source’s description.
- Forum: Reddit r/Peptides, u/beaubafett78 (comment, 2026-02-11)
- Forum: Reddit r/Peptides, u/beaubafett78 (comment, 2026-02-11)
- Forum: Reddit r/Peptides, u/beaubafett78 (comment, 2026-02-11)
- Forum: Reddit r/Peptides, u/beaubafett78 (comment, 2026-02-11)
Batch: 5 mL
Ingredients and methodHide the recipe
Ingredients
| Ingredient | Amount | Role |
|---|---|---|
| Semax | 5 or 10 mg | Active |
| Sterile saline ampule (1 mL to dissolve, the other 4 mL into the bottle) | 5 mL | Liquid |
Method
- Open the ampule and draw 1 mL into a syringe; use it to dissolve the peptide in its vial.
- Pour the other 4 mL of the ampule into the spray bottle.
- Draw the dissolved peptide back out of the vial and add it to the spray bottle.
- Work out how much each spray holds and do the maths for the daily dose.
- How it’s used
- About 750–1,000 mcg a day; the poster uses it 3–4 times a week.
- Per dose
- Just under 4 sprays = about 1,000 mcg with the poster’s bottle (stated; spray volume not given).
- Preservative
- None (plain saline).
- Poster reported no sting.
Sources (4)
-
ForumReddit r/Peptides, u/beaubafett78 (comment, 2026-02-11) (opens in a new tab)
“Pop the top and pull 1ml into your syringe and reconstitute, dump the rest (4ml) of the ampule into the spray bottle”
-
ForumReddit r/Peptides, u/beaubafett78 (comment, 2026-02-11) (opens in a new tab)
“That gives you an easy 5mg/5ml or 10mg/5ml ratio to work with.”
-
ForumReddit r/Peptides, u/beaubafett78 (comment, 2026-02-11) (opens in a new tab)
“it’s just under 4 sprays to technically hit 1000mcg.”
-
ForumReddit r/Peptides, u/beaubafett78 (comment, 2026-02-11) (opens in a new tab)
“No issues, no sting, 3-4 times a week.”
10 mg in 3 mL with preserved store-bought saline spray: 333 mcg per spray
Original source-specific report. Collection checked 30 Sep 2026; presentation annotated 2026-10-01. Source date and reporter identity are not separately verified. Exact form and route are limited to the source’s description.
- Forum: Reddit r/Peptides, u/Zealousideal-Lab4945 (comment, 2025-08-24)
- Forum: Reddit r/Peptides, u/Zealousideal-Lab4945 (comment, 2025-08-24)
- Forum: Reddit r/Peptides, u/Zealousideal-Lab4945 (comment, 2025-08-24)
- Forum: Reddit r/Peptides, u/Zealousideal-Lab4945 (comment, 2025-11-13)
- Forum: Reddit r/Peptides, u/Zealousideal-Lab4945 (comment, 2025-11-13)
Batch: 3 mL
Ingredients and methodHide the recipe
Ingredients
| Ingredient | Amount | Role |
|---|---|---|
| Semax | 10 mg | Active |
| Sterile water (to dissolve) | 1 mL | Liquid |
| Amazon Basic Care saline nasal spray | 2 mL | Liquid / preservative |
Method
- Dissolve the peptide in 1 mL sterile water.
- Draw it out and put it in a new 10 mL Snoot bottle.
- Pour in store-bought saline nasal spray to the strength you want.
- Per dose
- 333 mcg per spray if the bottle sprays 0.1 mL (stated).
- Preservative
- From the store-bought saline spray.
- Keeps for
- Poster said they always used it up before they ‘would begin to worry about it’.
- The same poster described a general method ‘with any pep’: dissolve with 1 mL sterile water, then mix with 9 mL saline, which they said gives 0.5 mg per mL and 50 mcg per 0.1 mL spray; they added that you ‘can put it through a 4mm filter’ when transferring.
Not stated: how it’s used.
Sources (5)
-
ForumReddit r/Peptides, u/Zealousideal-Lab4945 (comment, 2025-08-24) (opens in a new tab)
“I used 1ml of sterile water to recon, then pulled it out and into a new 10ml Snoot bottle, and then you can pour in saline”
-
ForumReddit r/Peptides, u/Zealousideal-Lab4945 (comment, 2025-08-24) (opens in a new tab)
“I used Amazon basic nasal spray because it has some preservatives in it. I used 2ml for a concentration of 10mg/3ml.”
-
ForumReddit r/Peptides, u/Zealousideal-Lab4945 (comment, 2025-08-24) (opens in a new tab)
“If the bottle sprays .1ml per spray then each spray is 333mcg.”
-
ForumReddit r/Peptides, u/Zealousideal-Lab4945 (comment, 2025-11-13) (opens in a new tab)
“If you recon with 1ml of sterile water, then mix with 9ml saline, you’ve got .5mg/ml.”
-
ForumReddit r/Peptides, u/Zealousideal-Lab4945 (comment, 2025-11-13) (opens in a new tab)
“You can put it through a 4mm filter during this if you want.”
Compounding pharmacy Semax nasal (Tailor Made Compounding, US)
Original source-specific report. Collection checked 30 Sep 2026; presentation annotated 2026-10-01. Source date and reporter identity are not separately verified. Exact form and route are limited to the source’s description.
- Pharmacy: Tailor Made Compounding – product information table, Semax row (archived 21 Jan 2020)
- Forum: Reddit r/Peptides, u/Miramiya99 (post, 2025-12-12)
Batch: 3 mL
Ingredients and methodHide the recipe
Ingredients
| Ingredient | Amount | Role |
|---|---|---|
| Semax | 7,500 mcg per mL | Active |
- Per dose
- Not stated (spray or drop volume not given).
- Listed as ‘Nasal’ in the pharmacy’s January 2020 product table (doctor price $40); the table does not say whether it is a spray or drops.
- Tailor Made also listed Semax for injection at 1,000 mcg per mL.
- In 2025 a user said their compounding pharmacy shipped them nasal spray peptides including Semax (strength not given).
Not stated: how it’s used.
Sources (2)
-
“Nasal 7500mcg/ml 1 x 3ml $40 Neurogenic/Neurorestorative”
-
ForumReddit r/Peptides, u/Miramiya99 (post, 2025-12-12) (opens in a new tab)
“My peptide compounding pharmacy used to always use fast shipping or couriers, but recently shipped nasal spray peptides (DSIP, Oxytocin and Semax)”
How the method was put together, step by step with its sources
Editorial synthesis and reported strength comparison
This synthesized method combines source records. It has not been validated for preparation, sterility, compatibility, absorption or storage. Select a reported strength to inspect the arithmetic; no strength is selected for you.
100 mcg per spray
Makes 30 mL · about 295 sprays
Synthesized quantities and equipment
- 30 mg N-Acetyl Semax Amidate
- 30 mL bacteriostatic water or saline (posters differ)
- Liquid, which people disagree on: sterile saline (gentler, but no preservative), bacteriostatic water (preserved, but can burn), or a mix of the two.
- An empty metered nasal spray bottle. Many are sold as about 0.1 mL per press, but users report 0.05–0.2 mL, so check yours.
- New sterile syringes to measure and move liquid. On a U-100 insulin syringe, 100 units is 1 mL, so 1 unit is 0.01 mL.
- Alcohol prep pads, for the vial stopper and the spray tip.
Synthesized steps · not validated
- Check your pump first: spray a measured amount of water through it and count the presses. Users report about 0.05–0.2 mL per press.
- Wash your hands. Wipe the vial’s rubber stopper with an alcohol pad and let it dry before piercing it.
- With a new sterile syringe, add a little of the liquid slowly, aiming it down the inside wall of the vial, not onto the powder.
- Swirl or roll the vial gently until the powder dissolves; don’t shake it. Don’t use it if it’s cloudy, discoloured or has particles.
- Draw the dissolved peptide back into the syringe and add it gently to the spray bottle.
- Rinse the vial with a little more of the liquid and add that to the bottle too, so no peptide is left behind.
- Make the bottle up to the full batch volume with the rest of the liquid.
- Fit the pump and press it into the air until a fine mist appears; labels say 4–6 presses. Re-prime after 3–7 days unused.
- Label the bottle with the date you mixed it and its strength.
- Before each dose, blow your nose gently. Close one nostril and aim the tip toward the back of your head, not the middle wall.
- Labels differ: some say breathe in gently as you press, others hold your breath. Try not to sneeze or blow your nose straight afterwards.
- Afterwards, wipe the tip with an alcohol wipe, put the cap back on and keep the bottle upright.
- Each spray
- 100 mcg per 0.1 mL spray
- Keeps
- Fridge (2–8 °C), upright; don’t freeze. No tested shelf life: pharmacy defaults are 14 days unpreserved, 35 days preserved; saline dilutes bacteriostatic water’s preservative.
- How it’s used
- One press per nostril at a time: a nostril holds about 0.1 mL, at most 0.15 mL, and more runs out or is swallowed.
Good to know
- Bacteriostatic water contains 0.9–1.1% benzyl alcohol as a preservative. Its label covers mixing injections, not nasal use; EU labels warn of allergic reactions.
- Some use store-bought saline nasal spray for its preservatives: often benzalkonium chloride, which can irritate the nose with long use, and sometimes benzyl alcohol.
- Never tap water: it is not sterile, and CDC researchers warn against it for nasal rinsing.
This method is a guide put together from the sources cited for each item (drug labels, pharmacy and CDC rules, research reviews and community recipes); it is not anyone’s tested protocol. The method’s sources
Strengths people report
- 100 mcg per spray: 30 mg N-Acetyl Semax Amidate in 30 mL bacteriostatic water or saline (posters differ) (30 mL · about 295 sprays)
- 200 mcg per spray: 10 mg N-Acetyl Semax Amidate in 5 mL sterile saline (5 mL · about 45 sprays (with a 0.1 mL pump))
- 250 mcg per spray: 10 mg N-Acetyl Semax Amidate in 2 mL bacteriostatic water + 2 mL saline (4 mL · about 35 sprays)
- 333 mcg per spray: 10 mg N-Acetyl Semax Amidate in 1 mL sterile water + 2 mL store-bought saline nasal spray (3 mL · about 25 sprays)
What people report
-
User reports
Several users say bacteriostatic water stings or ‘burns’ in the nose; others say the sting is brief or absent.
-
User reports
One user took apart a saline-only spray pump after 2 months and found grey-black deposits through the valve despite cleaning.
Source: Reddit r/Peptides, u/9to5grinder (2026-09-06) (opens in a new tab)
-
User reports
One user said a peptide nasal spray ‘just gave me a sinus infection’.
Source: Reddit r/Peptides, u/EducationalReply6493 (2026-09-23) (opens in a new tab)
See all 6Show fewer
-
User reports
One user got morning sneezing fits about a week into a home-made Selank/Semax spray made with sodium chloride and wondered whether bacteria in the bottle were the cause.
Source: Reddit r/Peptides, u/PlatinumEstates (2026-01-14) (opens in a new tab)
-
User reports
A user who mixed Semax and Selank with bacteriostatic water reported eye irritation for a couple of days.
Source: Reddit r/Nootropics, u/mdav97 (2025-09-10) (opens in a new tab)
-
User reports
A few users report patchy hair loss while using Semax spray daily; others report none.
Source: Reddit r/Nootropics – ‘NA-Semax: the holy grail’ (2020) (opens in a new tab)
Research notes
Form confusion is the main problem: most published protocols, clinic products and the FDA review concern plain Semax, and many forum users with ‘Semax’ research vials don’t say which form they have. Nothing specific to NA-Semax Amidate has ever been studied. Amounts per spray differ between products (100, 200, 240, 300 mcg; the clinic spray’s 7,500 mcg per mL has no stated spray size), so ‘sprays’ are not comparable. Users disagree on whether the amidate is stronger or weaker than plain Semax and whether nasal or injection works better. The FDA eye-pain report and the advisory vote both concern plain Semax.
Nasal spray
Bottle output varies (0.1–0.15 mL, and one bottle 0.15–0.20 mL), so ‘mcg per spray’ is only right for the poster’s own bottle; users advise spraying 10 mL of water through and counting. Storage advice ranges from ‘reconstitute one week at a time’ to a vendor’s claim (u/MisterYouAreSoDumb, 2016) that Semax spray kept 1 year at about 22 °C (72 °F) and 2 years in the fridge – unverified. Users disagree whether store-bought ‘Amazon’ saline spray contains benzyl alcohol; the DailyMed label for the same type of 0.65% saline spray lists both benzalkonium chloride and phenylcarbinol (benzyl alcohol). One user claimed N-acetyl amidate forms already contain preservatives; no source supports this.
What it is. In plain English.
The listed N-acetyl Semax amidate is a modified seller product; evidence and Russian product status for plain Semax do not establish its identity, effects or approval.
This entry names N-acetyl Semax amidate, a capped modified form. Plain Semax is a different heptapeptide product registered as nasal drops in Russia. Research or product status for plain Semax cannot be transferred to this amidate form.
Plain Semax has been studied for neurological effects, and proposed mechanisms include BDNF-related and neurotransmitter signaling. This entry is the N-acetyl amidate form; findings about plain Semax do not establish that this modified form has the same effects.
Reviewed 2026-10-01.
Structure. Nothing to show yet.
No experimental 3D structure of Semax or N-acetyl Semax amidate is in the .
Its first four amino acids, MEHF, occur inside the hormones ACTH and α-MSH bound to their receptors, but those pictures show the hormones, not Semax.
Checked 30 Sep 2026.
Specs. The facts at a glance.
- Length
- 7 amino acids
- Sequence
- Ac-MEHFPGP-NH₂
- Sold as
- N-Acetyl Semax Amidate 10 mg
- In blends
- Selank / Semax10 mg / 10 mg per vial
- Regulatory status
- Plain Semax is registered in Russia as nasal drops. The listed N-acetyl amidate is a distinct form with no approval established. A July 2026 PCAC recommendation concerned plain Semax free base/acetate for possible 503A list inclusion; it is not FDA drug approval or a final listing decision.
- Also known as
- Modified Semax
Sources. What this page rests on.
Every source behind this page, with what it says and when it was checked, is in the Source library.
N-Acetyl Semax Amidate in the Source library
Original overview and research notes · annotated 2026-10-01
Historical collection text. Read alongside the claim corrections above; identity or evidence qualifications may supersede this wording.
Focus, memory and brain protection
A modified version of Semax, a nasal nootropic used as medicine in Russia.
Semax is a synthetic seven-amino-acid chain: a fragment of the hormone ACTH (4–7) with Pro-Gly-Pro added to slow breakdown. The N-acetyl amidate form caps both ends, which sellers say makes it last longer. That modified form hasn’t been studied clinically.
It raises BDNF, a protein that supports neuron growth and learning, and affects dopamine and serotonin systems. Despite its ACTH origin, it doesn’t act as a stress hormone.
Russian clinical studies report benefits for stroke recovery and cognitive function. They’re mostly small and not published in major Western journals. Animal studies support neuroprotective effects.
Original status record: Approved in Russia as nasal drops for stroke and cognitive disorders, but not in the US or EU; the N-acetyl amidate form isn’t approved anywhere. In July 2026 FDA advisers voted to recommend allowing compounding of plain Semax; the FDA has not decided.