NAD+
Taken for energy and healthy aging
Nicotinamide adenine dinucleotide, not a peptide
Match every finding to its exact substance, formulation, route and study population. A related molecule or approved medicine does not validate a catalog vial.
Small molecule, not a peptide
At a glance
Why people take it. And what backs it up.
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Boost energy
People report it, not studied
The most common use: shots or IV drips to feel more energetic and less run-down.
Sources (3)
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“Clean energy that gets me going almost immediately, no crash.”
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“wrapping up my NAD+, Motsc and 5amino cycle (used for energy + body recomp)”
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ClinicWalnut Creek Aesthetics (clinic): NAD+ injection standard operating procedure (opens in a new tab)
“Restoring NAD+ levels improves energy, supports cellular repair, and promotes longevity.”
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Slow aging
People report it, not studied
Longevity clinics sell drips and shots to restore NAD+ levels that fall with age; animal anti-aging studies used oral precursors, not NAD+.
Sources (4)
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ClinicClarus Health (clinic, San Francisco): NAD+ IV therapy (opens in a new tab)
“The problem is that NAD+ levels decline significantly with age, chronic stress, poor sleep, and illness — and oral supplementation replaces only a fraction of what’s lost.”
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ClinicSanté Clinics (medical anti-aging clinic): NAD+ and longevity (opens in a new tab)
“Intravenous infusions raise its availability directly and can support cellular energy, mental clarity and repair processes within an anti-aging plan supervised by qualified doctors.”
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StudyMills et al., Cell Metabolism, 2016 (PubMed abstract) (opens in a new tab)
“Remarkably, NMN effectively mitigates age-associated physiological decline in mice.”
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StudyZhang et al., Science, 2016 (PubMed abstract) (opens in a new tab)
“We furthermore demonstrate that NR delays senescence of neural SCs and melanocyte SCs and increases mouse life span.”
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Clear brain fog
People report it, not studied
People take it, often as an IV drip, hoping for sharper focus and less brain fog.
Sources (2)
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ClinicClarus Health (clinic, San Francisco): NAD+ IV therapy (opens in a new tab)
“If you’re struggling with brain fog, fatigue, or fading focus — your cells are telling you something. IV NAD+ delivers directly where it’s needed”
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ClinicBeverly Hills Rejuvenation Center (clinic): NAD+ injection dosage guide (opens in a new tab)
“Many patients pursue NAD+ injections for subjective energy, mental clarity, or a sense of recovery.”
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Support addiction recovery
Clinic claims; no controlled trials
Detox clinics run IV drips hoping to ease withdrawal and cravings during recovery from drugs or alcohol.
Sources (4)
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“It will also ease the discomfort of withdrawal, reduce cravings, produce a mind and body cellular restoration, and speed up the detox process.”
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ClinicDrip Hydration (mobile IV clinic): NAD IV therapy for drug addiction (opens in a new tab)
“Nicotinamide adenine dinucleotide (NAD) IV treatments can help you beat addiction and avoid relapse.”
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ReviewBraidy et al., Antioxidants, 2020 (PubMed abstract) (opens in a new tab)
“Since clinical studies showing the use of NAD+ for the treatment of addiction are limited”
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“Larger randomized double-blinded placebo-controlled studies are needed to elucidate further the significance of the results presented in this study.”
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Evidence briefing
Sources checkedWhat the sources support.
NAD+ is a cellular coenzyme, not a peptide. The opened 2019 IV pilot randomized 11 healthy men: eight received NAD+ and three saline, with only short-term metabolite measurements. This is a small infusion study, not proof of anti-aging or clinical benefit. FDA has warned about food-grade NAD+ ingredients used in sterile injections and reports of severe chills, shaking, vomiting and fatigue consistent with endotoxin exposure. No FDA-approved NAD+ drug for wellness or longevity was identified.
Results from oral NAD+ precursors such as NR/NMN do not establish benefit from infusing the native coenzyme; the IV evidence is sparse and product sterility/endotoxin quality is a separate serious concern.
The research. What’s been studied.
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Human evidence
Cellular energy chemistry
NAD+ carries electrons in metabolic reactions that produce ATP; this normal biological role is not proof that supplementation increases energy or health in a person.
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Animal & lab studies
DNA-repair biology
NAD+ is used by PARP enzymes in DNA-repair processes; a clinical benefit from NAD+ injections has not been established.
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Animal & lab studies
Sirtuin research
Sirtuins use NAD+ and are studied in aging biology, mainly in experimental research; this does not show that NAD+ treatment slows human aging.
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Early human studies
Precursor studies
Oral NR and NMN can raise some NAD-related blood measures in human studies, but they are not the same as IV NAD+ and health outcomes remain mixed.
What do these labels mean?
- Human evidence: approved-drug data or larger human studies, within the populations and uses studied.
- Early human studies: small, preliminary or limited human studies.
- Animal & lab studies: animal or laboratory findings that do not establish effects in people.
- Unverified: a proposed use or claim without verified supporting clinical evidence.
How each claim was reviewed (4)
Reported effects and evidence.
A citation may support, qualify or correct a claim. Read the study scope; a laboratory result is not an established benefit in people. Each review covers a claim as it was first collected; the tiles above show the collection’s current wording.
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Unknown — no direct primary source verified. Established endogenous biochemical role; supplementation inference unsupported
Review of “Powers energy production”
NAD+ is an endogenous metabolic cofactor; this biochemical role is not evidence that supplemental NAD+ improves vitality.
Limitations and applicability. NAD+ being essential does not establish that administering additional NAD+ improves energy in people.
Unknown: no direct primary source verified. This does not mean that no research exists.
Original collected claim and review history
Original title: Powers energy production
Every cell needs NAD+ to turn food into ATP.
Original label: proven. Preserved for traceability; the reviewed wording above qualifies this record.
Record nad:benefit:1 · Annotated 2026-10-01 · Review method
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Preclinical endogenous NAD+ / DNA-repair mechanism
Review of “Supports DNA repair”
NAD+ participates in PARP1-related DNA-repair mechanisms; human benefit from administered NAD+ is unestablished by these experiments.
Limitations and applicability. Mouse and molecular NAD+–PARP1 findings do not establish clinical DNA-repair benefits of injected NAD+.
Original collected claim and review history
Original title: Supports DNA repair
Fuels PARP enzymes that repair damaged DNA.
Original label: animal. Preserved for traceability; the reviewed wording above qualifies this record.
Record nad:benefit:2 · Annotated 2026-10-01 · Review method
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Biochemical enzyme experiments
Review of “Activates sirtuins”
Sirtuin-family enzymes use NAD+; this molecular role does not establish a human anti-aging benefit from NAD+ products.
Limitations and applicability. NAD dependence of sirtuin activity is not evidence that an NAD+ product extends human healthspan.
Original collected claim and review history
Original title: Activates sirtuins
Sirtuins are proteins linked to healthy aging in animal studies.
Original label: animal. Preserved for traceability; the reviewed wording above qualifies this record.
Record nad:benefit:3 · Annotated 2026-10-01 · Review method
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Human oral precursor biomarker trials; formulation mismatch
Review of “Restores falling levels”
Oral NR trials raised blood NAD+ metabolites. Do not transfer that finding to injectable NAD+ or equate it with established healthy-aging benefit.
Limitations and applicability. Oral NR/NMN are different interventions from infused/injected NAD+; higher blood biomarker levels are not proven health benefit.
Original collected claim and review history
Original title: Restores falling levels
Levels drop with age; oral precursors (NR, NMN) reliably raise them in people, though health benefits are modest so far.
Original label: early. Preserved for traceability; the reviewed wording above qualifies this record.
Record nad:benefit:4 · Annotated 2026-10-01 · Review method
The research in detail
Evidence category
Small or older human studies
NAD+ is biologically essential, but that does not show that extra NAD+ improves health or slows aging. One small IV pilot enrolled 11 men, with eight receiving NAD+ and three saline, and measured short-term blood and urine metabolites. Much human NAD research concerns oral precursors such as NR and NMN, which are different interventions. FDA has warned about food-grade NAD+ used in sterile injections and reported reactions consistent with exposure.
Evidence is assessed claim by claim above. Regulatory approval, study design, findings and relevance are separate questions; there is no single evidence score.
Risks and unknowns. What to know first.
Collected cautions follow. These are not an exhaustive or independently validated safety assessment; claim references do not automatically support every caution.
- Given too fast, it causes nausea, cramping, chest tightness, flushing and headache.
- Effects of long-term high dosing are unknown.
What people report
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Study
In a retrospective study of a commercial drip clinic, all 6 clients given 500 mg NAD+ drips into a vein reported moderate to severe abdominal cramping, diarrhea, nausea, vomiting, faster heart rate and chest pressure during the drip; symptoms stopped when it ended.
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Study
A compounding-education review notes that safety and effectiveness data for injection-into-muscle protocols are unavailable, and that human dosing evidence is limited mainly to case reports.
Source: Fagron Academy: “NAD+: A Review on Dosing” (June 2025) (opens in a new tab)
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User reports
A podcast listener reported that his wife developed chest pain lasting at least two weeks after four NAD treatments on consecutive days.
Source: Ben Greenfield podcast page: “What Are Peptides...” (reader comments) (opens in a new tab)
Show all 4 cautionsShow fewer cautions
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User reports
Flushing is common with NAD+ injections; one user says injecting more slowly reduced it.
Source: Reddit r/Peptides: “MOTS-C histamine responses” (September 2026) (opens in a new tab)
Published dosing records. What the trials used.
Historical label and trial records for the named product, route, population and indication. These records are not a personal schedule or a validated regimen for a catalog vial. Source attribution does not establish applicability.
6-hour infusion study
Small pilot study in healthy men tracking NAD+ in blood and urine during a long drip (Frontiers in Aging Neuroscience, 2019)
Source: Grant et al., Frontiers in Aging Neuroscience, 2019 (opens in a new tab)
- Over 6 hours
- 750 mg
- How
- Into a vein (drip)
- How often
- One infusion, run over 6 hours
- How long
- A single 6-hour infusion
- Eleven healthy men took part: 8 got NAD+ and 3 got a saltwater drip.
- No side effects were seen during the infusions.
- The authors picked the dose because it matched a common regimen at wellness clinics.
- The drip ran at about 2 mg per minute.
Heart failure trial
Randomized, placebo-controlled trial in 180 adults with heart failure caused by coronary artery disease, on top of standard treatment (American Journal of Cardiovascular Drugs, 2026)
Source: Yu et al., American Journal of Cardiovascular Drugs, 2026 (opens in a new tab)
- Days 1–7
- 10 mg
- How
- Into a vein (drip)
- How often
- Once a day
- How long
- 7 days in the trial
- Each dose was mixed into 100 mL of glucose or salt solution and dripped in over about 40–60 minutes.
- The NAD+ product used carries a Chinese drug approval number.
- Drip rate no faster than 200 mL per hour.
Hearing-loss trial
Small randomized trial in 38 people treated in hospital for sudden hearing loss, on top of standard treatment (The Laryngoscope, 2026)
Source: Gao et al., The Laryngoscope, 2026 (opens in a new tab)
- Each day in hospital
- 5 mg
- How
- Into a muscle
- How often
- Once a day
- How long
- During the hospital stay (median 7 days, range 4–13 days)
- Each 5 mg dose was given in 2 mL of saltwater.
- The comparison group got standard treatment only, with no placebo injection.
These doses were tested in clinical trials under medical supervision. They aren’t approved for use, and a research vial is not the product that was tested.
Community protocols. Reported online. Not clinically validated.
Community reports Collection date: 30 Sep 2026NAD+ is used three main ways: injections under the skin at clinics or at home (usually 50–100 mg, one to three times a week, started at 20–50 mg because it causes flushing and discomfort); intravenous drips of about 250–1,000 mg run slowly over about 1.5–5 hours; and multi-day intravenous “detox” programs of 500–1,500 mg a day.
More about this
Nasal sprays range from about 25 mg to 120 mg a day.
Injection under the skin (wellness / anti-aging)
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
- Clinic: Walnut Creek Aesthetics (clinic): NAD+ injection standard operating procedure
- Clinic: Walnut Creek Aesthetics (same page)
- Clinic: Beverly Hills Rejuvenation Center (clinic): NAD+ injection dosage guide
- Clinic: Beverly Hills Rejuvenation Center (same page)
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- 50–100 mg per shot (started at 20–50 mg)
- How often
- Once a week up to three times a week
- Route
- Injection under the skin (belly, thigh or back of the arm)
- How long
- Ongoing; one clinic reviews the dose every 4 weeks
- Ramp
- 50 mg once a week for 4 weeks, then 100 mg if tolerated (one clinic’s standard procedure); another clinic describes an optional loading phase of 100–200 mg daily for 7–10 days
- Mixing
- Compounded or research vials; at 200 mg/mL, 0.25 mL is about 50 mg and 0.5 mL about 100 mg
- Started low because injections can cause flushing, cramping, nausea and injection-site pain.
Sources (4)
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ClinicWalnut Creek Aesthetics (clinic): NAD+ injection standard operating procedure (opens in a new tab)
“Start with 50 mg (0.5 mL) subcutaneously once weekly for 4 weeks.”
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ClinicWalnut Creek Aesthetics (same page) (opens in a new tab)
“Maximum dose: 100 mg once weekly.”
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ClinicBeverly Hills Rejuvenation Center (clinic): NAD+ injection dosage guide (opens in a new tab)
“started low — around 20–50 mg per injection — and titrated up toward roughly 50–100 mg, given 1–3 times per week”
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ClinicBeverly Hills Rejuvenation Center (same page) (opens in a new tab)
“loading phase of roughly 100–200 mg daily for 7–10 days”
Intravenous drip at a clinic
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
- Review: Reyna et al. 2026: retrospective tolerability study of commercial IV NAD+ vs nicotinamide riboside (PMC)
- Clinic: IV Elements (IV clinic, New York / New Jersey / Pennsylvania): addiction programs
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- About 250–1,000 mg per drip (500 mg is typical)
- How often
- Single sessions, or several days in a row (for example 4 daily 500 mg drips as a “loading” course)
- Route
- Drip into a vein (intravenous)
- Timing
- Run slowly; sessions last about 1.5–5 hours, and the drip is slowed when symptoms appear
- Cycle
- Some continue with a drip every 4–6 weeks (one case report)
- Mixing
- Mixed into a saline bag by clinic staff
- Discomfort during the drip is so common that patients in one study set their own drip speed.
Sources (2)
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“Clients controlled their own infusion rate based on tolerance.”
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“500mg BR+ NAD over 3-4 hours”
Show all 7 protocolsShow fewer protocols
Multi-day intravenous addiction or detox program (“BR+NAD”)
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
- Clinic: IV Elements (IV clinic): addiction programs
- Clinic: IV Elements (same page)
- Review: Fagron Academy (compounding-supplier education, Sarah Taylor PharmD): “NAD+: A Review on Dosing” (June 2025)
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- 500–1,500 mg a day
- How often
- One drip a day
- Route
- Drip into a vein (intravenous)
- Timing
- Each drip over about 3–5 hours
- How long
- About 4–15 days in a row
- Cycle
- Some programs then taper to twice a week for a month, then twice a month
- Ramp
- Some start high (for example 1,500 mg on day 1) and step down
- Sold for alcohol, opioid, benzodiazepine and methadone withdrawal, often with vitamin drips and other add-ons; programs cost thousands of dollars.
Sources (3)
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ClinicIV Elements (IV clinic): addiction programs (opens in a new tab)
“750mg BR+ NAD over 3-4 hours”
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ClinicIV Elements (same page) (opens in a new tab)
“For NAD/BR+ Therapy to truly work, it usually requires one infusion per day for a period of ten days.”
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“500-1000mg of NAD+ in 300mL of normal saline typically with a loading period of daily dosing for four days, followed by a taper period of twice weekly dosing”
Nasal spray
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
- Vendor: Best365Labs (vendor): NAD+ nasal spray
- Review: Fagron Academy: “NAD+: A Review on Dosing” (Parkinson’s case report)
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- About 25 mg a day (retail spray) up to 120 mg a day (compounded, in a case report)
- How often
- Once or twice a day
- Route
- Nasal
- Retail sprays contain far less than compounded clinic sprays.
Sources (2)
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VendorBest365Labs (vendor): NAD+ nasal spray (opens in a new tab)
“Each spray delivers approximately 6.67 mg NAD+, for a daily dose of about 26.7 mg NAD+”
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ReviewFagron Academy: “NAD+: A Review on Dosing” (Parkinson’s case report) (opens in a new tab)
“received 30mg in each nostril twice daily (120mg total per day) for ongoing treatment.”
Pre-mixed with MOTS-c, or small twice-weekly shots
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
- Forum: Reddit r/Peptides: “Flushing with NAD+/MOTS C?” (September 2026)
- Forum: Reddit r/Peptides: “Advice” (September 2026)
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- About 30–90 mg NAD+ per shot
- How often
- Once or twice a week
- Route
- Injection under the skin
- Timing
- Morning
- Ramp
- Stepped up gradually because of flushing
- Mixing
- Pre-mixed NAD+/MOTS-c vials (one: 20 units = 60 mg NAD+ + 0.5 mg MOTS-c)
Sources (2)
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ForumReddit r/Peptides: “Flushing with NAD+/MOTS C?” (September 2026) (opens in a new tab)
“I was ramping from 10 to 20 units every Friday for my first two weeks of NAD+/MOTS C (pre-mixed subcutaneous).”
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ForumReddit r/Peptides: “Advice” (September 2026) (opens in a new tab)
“- 33mg NAD+ in the morning”
Injection into a muscle
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- 25–50 mg to start, up to 100 mg
- How often
- Up to twice a week
- Route
- Into a muscle
- Described as anecdotal, with no safety or effectiveness data.
Sources (1)
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ReviewFagron Academy: “NAD+: A Review on Dosing” (June 2025) (opens in a new tab)
“Anecdotally, NAD+ is sometimes used IM at 25-50mg to start, with doses up to 100mg per IM injection in some protocols dosed up to twice weekly.”
Oral NAD+
Collected report · exact form and route are only as reported below. Source date and reporter identity are not separately verified. Collection checked 30 Sep 2026; presentation annotated 2026-10-01.
Frequency labels describe this collection, not a measured population. Contradictory records remain available.
Details and sourcesHide details
- Dose
- 600 mg a day (one user)
- How often
- Daily
- Route
- Oral
- A compounding-education review says oral NAD+ is generally not recommended because it is poorly absorbed.
Sources (1)
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ForumReddit r/Peptides: “Tirze + NAD+ (oral) + MOTS question” (September 2026) (opens in a new tab)
“Oral NAD+ - 600mg per day”
Where the numbers come from
The intravenous protocols trace largely to addiction-treatment clinics using the “BR+NAD” (Brain Restoration) approach linked to NAD Research, Inc., which a compounding-education review cites for its daily-drip loading courses. Injection doses are clinic convention, and published human evidence is mostly case reports.
Home-made sprays and serums
Home-made nasal spray. Community recipes. Not tested.
Original source recordsHide
NAD+ nasal sprays are sold by at least one US compounding pharmacy (Empower, 300 mg per mL) and by vendors (for example 1,000 mg per bottle, 10 mg per spray). Users report doses such as three 10 mg sprays a day, 20 mg a day, or one spray per nostril each morning. No home-made recipe was found. How much is absorbed is unknown.
Best evidence: Compounding pharmacy product (Empower Pharmacy, 503A, 15 mL, 300 mg per mL, refrigerated). Vendor products. Only animal research on intranasal NAD found (mouse smell-loss study, 2026); no human trials.
Ready-made vendor NAD+ sprays (for comparison)
Original source-specific report. Collection checked 30 Sep 2026; presentation annotated 2026-10-01. Source date and reporter identity are not separately verified. Exact form and route are limited to the source’s description.
- Forum: Reddit r/Peptides, u/jordanvaca254 (post, 2023-12-07)
- Forum: Reddit r/Peptides, u/bigbabyqueso (post, 2025-08-06)
- Forum: Reddit r/Peptides, u/HectorSharpPruners (post, 2023-11-15)
Ingredients and methodHide the recipe
Ingredients
| Ingredient | Amount | Role |
|---|---|---|
| NAD+ | 1,000 mg per bottle (10 mg per spray) or 500 mg per bottle | Active |
- How it’s used
- Reported: 3 sprays a day; 1 spray per nostril each morning; 20 mg a day.
- Per dose
- 10 mg per spray (1,000 mg bottle, stated).
Sources (3)
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ForumReddit r/Peptides, u/jordanvaca254 (post, 2023-12-07) (opens in a new tab)
“Purchased NAD+ from amino, 1000mg bottle, 10mg per spray. I’ve been doing three sprays a day”
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ForumReddit r/Peptides, u/bigbabyqueso (post, 2025-08-06) (opens in a new tab)
“NAD+ 500mg Nasal Spray - Every Morning One Spray Per Nostril”
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ForumReddit r/Peptides, u/HectorSharpPruners (post, 2023-11-15) (opens in a new tab)
“NAD+ 20mg/day via nasal spray two weeks ago.”
Compounding pharmacy NAD+ nasal spray (Empower Pharmacy)
Original source-specific report. Collection checked 30 Sep 2026; presentation annotated 2026-10-01. Source date and reporter identity are not separately verified. Exact form and route are limited to the source’s description.
- Pharmacy: Empower Pharmacy – NAD+ Nasal Spray product page
- Pharmacy: Empower Pharmacy – NAD+ Nasal Spray product page
- Pharmacy: Empower Pharmacy – Nasal Spray Instructions (PDF, 2025)
- Pharmacy: Empower Pharmacy – Nasal Spray Instructions (PDF, 2025)
Batch: 15 mL
Ingredients and methodHide the recipe
Ingredients
| Ingredient | Amount | Role |
|---|---|---|
| NAD+ | 300 mg per mL | Active |
- How it’s used
- As prescribed. Empower’s general nasal-spray instructions: blow your nose; prime until a fine mist appears; close one nostril and aim toward the back of the head, not the septum; wipe the nozzle and store upright.
- Storage
- Refrigerator, 2–8 °C; do not freeze; protect from light.
- Keeps for
- Discard after the pharmacy’s beyond-use date.
- Listed as available through Empower’s 503A (prescription) pharmacy.
Not stated: the amount per dose.
Sources (4)
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PharmacyEmpower Pharmacy – NAD+ Nasal Spray product page (opens in a new tab)
“NAD+ Nasal Spray (15 mL) 300 mg/mL”
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PharmacyEmpower Pharmacy – NAD+ Nasal Spray product page (opens in a new tab)
“Store this medication in a refrigerator between 36°F to 46°F (2°C – 8°C). Do not freeze. Protect from light.”
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PharmacyEmpower Pharmacy – Nasal Spray Instructions (PDF, 2025) (opens in a new tab)
“press down on the nozzle and spray into the air away from yourself and others until a fine mist appears.”
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PharmacyEmpower Pharmacy – Nasal Spray Instructions (PDF, 2025) (opens in a new tab)
“Wipe the nozzle with a clean tissue and replace the cap on the nozzle. Store the bottle upright.”
Intranasal NAD in mice (reference, not a home recipe)
Original source-specific report. Collection checked 30 Sep 2026; presentation annotated 2026-10-01. Source date and reporter identity are not separately verified. Exact form and route are limited to the source’s description.
Ingredients and methodHide the recipe
Ingredients
| Ingredient | Amount | Role |
|---|---|---|
| NAD | dose not given in the abstract | Active |
- How it’s used
- Mice with chemically induced loss of smell.
Sources (1)
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“restoring olfactory function in an anosmia mouse model.”
How the method was put together, step by step with its sources
Editorial synthesis and reported strength comparison
This synthesized method combines source records. It has not been validated for preparation, sterility, compatibility, absorption or storage. Select a reported strength to inspect the arithmetic; no strength is selected for you.
No amounts were found in the reviewed recipe records for this one. What was found is under “Original source records”.
The general method for home-made nasal sprays, with its sources
Good to know
- Bacteriostatic water contains 0.9–1.1% benzyl alcohol as a preservative. Its label covers mixing injections, not nasal use; EU labels warn of allergic reactions.
- Some use store-bought saline nasal spray for its preservatives: often benzalkonium chloride, which can irritate the nose with long use, and sometimes benzyl alcohol.
- Never tap water: it is not sterile, and CDC researchers warn against it for nasal rinsing.
What people report
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User reports
Users who tried both felt injections more than the nasal spray; how much NAD+ a spray delivers is described as ‘genuinely uncertain’.
Source: Reddit r/Nootropics – ‘NAD nasal spray vs subcutaneous injections’ (2026) (opens in a new tab)
On the skin: home-made serums. Community recipes. Not tested.
Original source recordsHide
NAD+ is sold as a cosmetic ingredient for serums and creams. One supplier gives a use level of 0.5–1% and warns it breaks down with heat and alkaline pH. Users mention adding NAD+ to home-made peptide serums but give no amounts.
Best evidence: Supplier information only. No human topical studies of NAD+ itself were found.
Supplier use level for NAD+ in a serum or cream
Original source-specific report. Collection checked 30 Sep 2026; presentation annotated 2026-10-01. Source date and reporter identity are not separately verified. Exact form and route are limited to the source’s description.
- Supplier: Bulk Naturals (supplier): Coenzyme NAD+
- Supplier: Bulk Naturals: Coenzyme NAD+
- Supplier: Bulk Naturals: Coenzyme NAD+
- Forum: Reddit r/Peptides, u/Difficult-Status-318 post (Jun 2025)
- Forum: Reddit r/Peptides, u/kvadratas2 (Jun 2025)
Batch: per 100% (any batch size)
Ingredients and methodHide the recipe
Ingredients
| Ingredient | % | Role |
|---|---|---|
| NAD+ (nicotinamide adenine dinucleotide) powder | 0.5–1% | Active |
| Water-based serum or cream base | Base |
Method
- Dissolve fully (the supplier suggests vortex mixing or a sonicator).
- Formulate at cool temperatures; NAD+ breaks down from about 38 °C and in alkaline pH.
- Storage
- Refrigerator; airtight, opaque packaging
- A Reddit user planning an all-in-one serum with NAD+ was told NAD+ and glutathione degrade quickly in solution.
Not stated: how it’s used.
Sources (5)
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SupplierBulk Naturals (supplier): Coenzyme NAD+ (opens in a new tab)
“Recommended Usage Rate: 0.5–1%”
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SupplierBulk Naturals: Coenzyme NAD+ (opens in a new tab)
“NAD+ degrades rapidly with heat (38C and up) and alkaline pH. We recommend storing your finished formulations in the refrigerator for maximum potency.”
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SupplierBulk Naturals: Coenzyme NAD+ (opens in a new tab)
“store finished products in airtight, opaque packaging.”
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ForumReddit r/Peptides, u/Difficult-Status-318 post (Jun 2025) (opens in a new tab)
“Snap 8 Hyaluronic Acid TB 500 BPC 157 NAD+ Glutathione”
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ForumReddit r/Peptides, u/kvadratas2 (Jun 2025) (opens in a new tab)
“Consider stability data for NAD+ and glutathione in solution; they degrade quickly.”
How the method was put together, step by step with its sources
Editorial synthesis and reported strength comparison
This synthesized method combines source records. It has not been validated for preparation, sterility, compatibility, absorption or storage. Select a reported strength to inspect the arithmetic; no strength is selected for you.
No amounts were found in the reviewed recipe records for this one. What was found is under “Original source records”.
The general method for home-made serums, with its sources
Good to know
- Use distilled water, not tap: tap water carries minerals, chlorine and microbes that can spoil the serum.
Research notes
NAD+ is not a peptide, and the evidence behind all of these protocols is thin. Clinic figures vary: one clinic’s standard procedure caps injections at 100 mg a week, while another clinic describes 100–200 mg daily loading. Home users often take NAD+ alongside several peptides and GLP-1 drugs.
Nasal spray
Empower lists its spray as 300 mg per mL, while vendor sprays are described per bottle or per spray without bottle volumes, so their strengths cannot be compared. No home-made NAD+ spray recipe was found (a 2025 ‘DIY’ question went unanswered). User claims of ‘20–40%’ nasal absorption are unsourced. The only intranasal NAD research found is in mice.
On the skin
Only one supplier’s use level was found. General web search engines were largely blocked during this research (captcha / degraded results), so a web-only product could have been missed.
What it is. In plain English.
A coenzyme involved in cellular energy reactions; evidence for wellness benefits from IV or injected NAD+ is very limited.
A molecule built from two nucleotides, made in the body from vitamin B3. “Buffered” means the pH is adjusted to reduce the sting of injection.
NAD+ carries electrons in the reactions that turn food into cellular energy (ATP). It’s also used up by DNA-repair enzymes (PARPs) and by sirtuins, proteins linked to aging. Tissue levels decline with age, which drives the interest in topping it up.
Reviewed 2026-10-01.
Structure. The same molecule, inside an enzyme.
Related structure
NAD+ (orange) bound inside SIRT5 (gray), a human enzyme that uses NAD+. The enzyme’s second copy in the crystal, its substrate peptides and a zinc ion are not drawn. The picture shows the molecule’s shape; it says nothing about what taking NAD+ does.
How it differs. This is the exact molecule, NAD+, but measured as a helper (a cofactor) sitting in an enzyme, not on its own. Its shape here is set by the enzyme’s pocket.
3RIY, Du et al., 2011. Rendered with Mol* (Sehnal et al., 2021) from RCSB PDB data (Berman et al., 2000).
, not measured
A computed model of free NAD+, on its own and not bound to any enzyme: a shape calculated from its atoms and bonds. Carbon atoms are orange, phosphorus a deeper orange, nitrogen blue and oxygen red; hydrogen atoms aren’t drawn. Its formula and bonds were checked against the molecule’s known structure.
Good to know
- NAD+ is flexible, so this is only one of the shapes it can take.
PubChem CID 5892. Rendered with Mol* (Sehnal et al., 2021).
Specs. The facts at a glance.
- Type
- coenzyme
- Sold as
- NAD+ 250 mg buffered
- NAD+ 500 mg buffered
- Regulatory status
- No FDA-approved NAD+ drug for wellness or longevity was identified. Clinics may offer compounded or other injectable products, but those are not FDA-approved treatments; FDA has warned about food-grade ingredients and endotoxin risks in sterile NAD+ injections.
- Also known as
- Nicotinamide adenine dinucleotide, not a peptide
Sources. What this page rests on.
Every source behind this page, with what it says and when it was checked, is in the Source library.
Original overview and research notes · annotated 2026-10-01
Historical collection text. Read alongside the claim corrections above; identity or evidence qualifications may supersede this wording.
Cellular energy and healthy aging
A coenzyme every cell uses for energy, popular as an anti-aging injection or IV drip.
A molecule built from two nucleotides, made in the body from vitamin B3. “Buffered” means the pH is adjusted to reduce the sting of injection.
NAD+ carries electrons in the reactions that turn food into cellular energy (ATP). It’s also used up by DNA-repair enzymes (PARPs) and by sirtuins, proteins linked to aging. Tissue levels decline with age, which drives the interest in topping it up.
Boosting NAD+ in mice improves metabolism and some signs of aging. Human research mostly uses oral precursors (NR, NMN), which reliably raise blood NAD+ but have shown modest or mixed health benefits. Injected or IV NAD+ has very little controlled research.
Original status record: Not an approved drug for these uses; offered by wellness clinics and sold as a research chemical.